Differential Contributions of MYCs to Insect Defense Reveals Flavonoids Alleviating Growth Inhibition Caused by Wounding in Arabidopsis

doi:10.3389/fpls.2021.700555

	Differential Contributions of MYCs to Insect Defense Reveals Flavonoids Alleviating Growth Inhibition Caused by Wounding in Arabidopsis
	Wang, Dan-Dan1,2 ; Li, Pai 1,3; Chen, Qiu-Yi 1,3; Chen, Xue-Ying1,2 ; Yan, Zi-Wei 1,3; Wang, Mu-Yang 1; Mao, Ying-Bo 1
	2021-07-13
发表期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
卷号	12
DOI	10.3389/fpls.2021.700555
摘要	In Arabidopsis, basic helix-loop-helix transcription factors (TFs) MYC2, MYC3, and MYC4 are involved in many biological processes, such as defense against insects. We found that despite functional redundancy, MYC-related mutants displayed different resistance to cotton bollworm (Helicoverpa armigera). To screen out the most likely genes involved in defense against insects, we analyzed the correlation of gene expression with cotton bollworm resistance in wild-type (WT) and MYC-related mutants. In total, the expression of 94 genes in untreated plants and 545 genes in wounded plants were strongly correlated with insect resistance, and these genes were defined as MGAIs (MYC-related genes against insects). MYC3 had the greatest impact on the total expression of MGAIs. Gene ontology (GO) analysis revealed that besides the biosynthesis pathway of glucosinolates (GLSs), MGAIs, which are well-known defense compounds, were also enriched in flavonoid biosynthesis. Moreover, MYC3 dominantly affected the gene expression of flavonoid biosynthesis. Weighted gene co-expression network analysis (WGCNA) revealed that AAE18, which is involved in activating auxin precursor 2,4-dichlorophenoxybutyric acid (2,4-DB) and two other auxin response genes, was highly co-expressed with flavonoid biosynthesis genes. With wounding treatment, the WT plants exhibited better growth performance than chalcone synthase (CHS), which was defective in flavonoid biosynthesis. The data demonstrated dominant contributions of MYC3 to cotton bollworm resistance and imply that flavonoids might alleviate the growth inhibition caused by wounding in Arabidopsis.
关键词	MYC transcription factors transcriptome plant resistance to insects WGCNA flavonoids
URL	查看原文
收录类别	SCIE
语种	英语
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:000678985900001
出版者	FRONTIERS MEDIA SA
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/127859
专题	生命科学与技术学院_博士生
通讯作者	Mao, Ying-Bo
作者单位	1.Chinese Acad Sci, Shanghai Inst Plant Physiol & Ecol, CAS Ctr Excellence Mol Plant Sci, CAS Key Lab Insect Dev & Evolutionary Biol, Shanghai, Peoples R China; 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China; 3.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Wang, Dan-Dan,Li, Pai,Chen, Qiu-Yi,et al. Differential Contributions of MYCs to Insect Defense Reveals Flavonoids Alleviating Growth Inhibition Caused by Wounding in Arabidopsis[J]. FRONTIERS IN PLANT SCIENCE,2021,12.
APA	Wang, Dan-Dan.,Li, Pai.,Chen, Qiu-Yi.,Chen, Xue-Ying.,Yan, Zi-Wei.,...&Mao, Ying-Bo.(2021).Differential Contributions of MYCs to Insect Defense Reveals Flavonoids Alleviating Growth Inhibition Caused by Wounding in Arabidopsis.FRONTIERS IN PLANT SCIENCE,12.
MLA	Wang, Dan-Dan,et al."Differential Contributions of MYCs to Insect Defense Reveals Flavonoids Alleviating Growth Inhibition Caused by Wounding in Arabidopsis".FRONTIERS IN PLANT SCIENCE 12(2021).