The careful control of Polo kinase by APC/C-Ube2C ensures the intercellular transport of germline centrosomes during Drosophila oogenesis

doi:10.1098/rsob.200371

	The careful control of Polo kinase by APC/C-Ube2C ensures the intercellular transport of germline centrosomes during Drosophila oogenesis
	Braun, Alexis Leah 1; Meghini, Francesco 1; Villa-Fombuena, Gema 2; Guermont, Morgane 1; Fernandez-Martinez, Elisa 1; Qian, Zhang3 ; Dolores Martin-Bermudo, Maria 2; Gonzalez-Reyes, Acaimo 2; Glover, David Moore 1; Kimata, Yuu1,3
	2021-06-30
发表期刊	OPEN BIOLOGY (IF:4.5[JCR-2023],5.9[5-Year])
EISSN	2046-2441
卷号	11 期号:6
DOI	10.1098/rsob.200371
摘要	A feature of metazoan reproduction is the elimination of maternal centrosomes from the oocyte. In animals that form syncytial cysts during oogenesis, including Drosophila and human, all centrosomes within the cyst migrate to the oocyte where they are subsequently degenerated. The importance and the underlying mechanism of this event remain unclear. Here, we show that, during early Drosophila oogenesis, control of the Anaphase Promoting Complex/Cyclosome (APC/C), the ubiquitin ligase complex essential for cell cycle control, ensures proper transport of centrosomes into the oocyte through the regulation of Polo/Plk1 kinase, a critical regulator of the integrity and activity of the centrosome. We show that novel mutations in the APC/C-specific E2, Vihar/Ube2c, that affect its inhibitory regulation on APC/C cause precocious Polo degradation and impedes centrosome transport, through destabilization of centrosomes. The failure of centrosome migration correlates with weakened microtubule polarization in the cyst and allows ectopic microtubule nucleation in nurse cells, leading to the loss of oocyte identity. These results suggest a role for centrosome migration in oocyte fate maintenance through the concentration and confinement of microtubule nucleation activity into the oocyte. Considering the conserved roles of APC/C and Polo throughout the animal kingdom, our findings may be translated into other animals.
关键词	ANAPHASE-PROMOTING COMPLEX CELL-CYCLE PERICENTRIOLAR MATERIAL OOCYTE DIFFERENTIATION CYTOPLASMIC DYNEIN MICROTUBULE MOTOR ORGANIZATION MEIOSIS PROTEIN APC/C
收录类别	SCIE
语种	英语
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
WOS记录号	WOS:000669513100001
出版者	ROYAL SOC
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/127662
专题	生命科学与技术学院_博士生生命科学与技术学院_PI研究组_Yuu Kimita组
通讯作者	Braun, Alexis Leah; Kimata, Yuu
作者单位	1.Univ Cambridge, Dept Genet, Cambridge CB2 3EH, England; 2.CSIC Univ Pablo Olavide JA, Ctr Andaluz Biol Desarrollo, Carretera Utrera Km 1, Seville 41013, Spain; 3.ShanghaiTech Univ, Sch Life Sci & Technol, 393 Middle Huaxia Rd, Shanghai 201210, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Braun, Alexis Leah,Meghini, Francesco,Villa-Fombuena, Gema,et al. The careful control of Polo kinase by APC/C-Ube2C ensures the intercellular transport of germline centrosomes during Drosophila oogenesis[J]. OPEN BIOLOGY,2021,11(6).
APA	Braun, Alexis Leah.,Meghini, Francesco.,Villa-Fombuena, Gema.,Guermont, Morgane.,Fernandez-Martinez, Elisa.,...&Kimata, Yuu.(2021).The careful control of Polo kinase by APC/C-Ube2C ensures the intercellular transport of germline centrosomes during Drosophila oogenesis.OPEN BIOLOGY,11(6).
MLA	Braun, Alexis Leah,et al."The careful control of Polo kinase by APC/C-Ube2C ensures the intercellular transport of germline centrosomes during Drosophila oogenesis".OPEN BIOLOGY 11.6(2021).