A suite of new Dre recombinase drivers markedly expands the ability to perform intersectional genetic targeting

doi:10.1016/j.stem.2021.01.007

	A suite of new Dre recombinase drivers markedly expands the ability to perform intersectional genetic targeting
	Han, Ximeng1,11 ; Zhang, Zhenqian 1; He, Lingjuan 1; Zhu, Huan 1; Li, Yan 1; Pu, Wenjuan 1; Han, Maoying11 ; Zhao, Huan 1; Liu, Kuo 1,12; Li, Yi 1,2; Huang, Xiuzhen 1; Zhang, Mingjun 1; Jin, Hengwei 1; Lv, Zan 1; Tang, Juan 1; Wang, Jinjin 3; Sun, Ruilin 3; Fei, Jian 3; Tian, Xueying 4; Duan, Shengzhong 5,6,7; Wang, Qing-Dong 8; Wang, Lixin 9,10; He, Ben 2; Zhou, Bin1,11,12,13
	2021
发表期刊	CELL STEM CELL (IF:19.8[JCR-2023],22.6[5-Year])
ISSN	1934-5909
EISSN	1875-9777
卷号	28 期号:6 页码:1160-+
DOI	10.1016/j.stem.2021.01.007
摘要	The use of the dual recombinase-mediated intersectional genetic approach involving Cre-loxP and Dre-rox has significantly enhanced the precision of in vivo lineage tracing, as well as gene manipulation. However, this approach is limited by the small number of Dre recombinase driver constructs available. Here, we developed more than 70 new intersectional drivers to better target diverse cell lineages. To highlight their applicability, we used these new tools to study the in vivo adipogenic fate of perivascular progenitors, which revealed that PDGFRa(+) but not PDGFRa(-)PDGFRb(+) perivascular cells are the endogenous progenitors of adult adipocytes. In addition to lineage tracing, we used members of this new suite of drivers to more specifically knock out genes in complex tissues, such as white adipocytes and lymphatic vessels, that heretofore cannot be selectively targeted by conventional Cre drivers alone. In summary, these new transgenic tools expand the intersectional genetic approach while enhancing its precision.
收录类别	SCIE
语种	英语
WOS研究方向	Cell Biology
WOS类目	Cell & Tissue Engineering ; Cell Biology
WOS记录号	WOS:000658938000018
出版者	CELL PRESS
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/127525
专题	生命科学与技术学院_硕士生生命科学与技术学院_特聘教授组_周斌组生命科学与技术学院_博士生
通讯作者	He, Ben; Zhou, Bin
作者单位	1.Chinese Acad Sci, Univ Chinese Acad Sci, Ctr Excellence Mol Cell Sci, State Key Lab Cell Biol,Shanghai Inst Biochem & C, Shanghai 200031, Peoples R China; 2.Shanghai Jiao Tong Univ, Shanghai Chest Hosp, Dept Cardiol, Shanghai, Peoples R China; 3.Shanghai Model Organisms Ctr Inc, Shanghai Engn Res Ctr Model Org, Shanghai, Peoples R China; 4.Jinan Univ, Coll Life Sci & Technol, Key Lab Regenerat Med, Minist Educ, Guangzhou, Peoples R China; 5.Shanghai Jiao Tong Univ, Sch Med, Shanghai Peoples Hosp 9, Lab Oral Microbiota & Syst Dis,Coll Stomatol, Shanghai 200125, Peoples R China; 6.Natl Clin Res Ctr Oral Dis, Shanghai Key Lab Stomatol, Shanghai 200011, Peoples R China; 7.Shanghai Res Inst Stomatol, Shanghai 200011, Peoples R China; 8.AstraZeneca, BioPharmaceut R&D, Biosci Cardiovasc Res & Early Dev Cardiovasc Rena, Gothenburg, Sweden; 9.Fudan Univ, Zhongshan Hosp, Dept Vasc Surg, Shanghai, Peoples R China; 10.Fudan Univ, Zhongshan Hosp, Xiamen Branch, Dept Vasc Surg, Xiamen, Peoples R China; 11.ShanghaiTech Univ, Sch Life Sci & Technol, 100 Haike Rd, Shanghai 201210, Peoples R China; 12.Univ Chinese Acad Sci, Sch Life Sci, Hangzhou Inst Adv Study, Hangzhou 310024, Peoples R China; 13.Chinese Acad Sci, Inst Stem Cell & Regenerat, Beijing 100101, Peoples R China
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Han, Ximeng,Zhang, Zhenqian,He, Lingjuan,et al. A suite of new Dre recombinase drivers markedly expands the ability to perform intersectional genetic targeting[J]. CELL STEM CELL,2021,28(6):1160-+.
APA	Han, Ximeng.,Zhang, Zhenqian.,He, Lingjuan.,Zhu, Huan.,Li, Yan.,...&Zhou, Bin.(2021).A suite of new Dre recombinase drivers markedly expands the ability to perform intersectional genetic targeting.CELL STEM CELL,28(6),1160-+.
MLA	Han, Ximeng,et al."A suite of new Dre recombinase drivers markedly expands the ability to perform intersectional genetic targeting".CELL STEM CELL 28.6(2021):1160-+.