上海科技大学知识管理系统

本发明公开了一种基于CRISPR技术的封闭式全自动核酸提取检测系统，包括囊袋式生物芯片和控制检测器。所述囊袋式生物芯片包括核酸萃取腔、一号清洗液腔、二号清洗液腔、三号清洗液腔、洗脱液腔、废液腔、扩增腔和CRISPR检测腔，分别用于存放核酸提取和检测所需的各种液体，各个腔体之间通过微流道相连。所述控制检测器包括推杆控制器、可控交变电磁铁和荧光检测器，推杆控制器用于控制液体流向，可控交变电磁铁用于搅拌和吸附磁珠，荧光检测器用于核酸的CRISPR发光检测。本发明实现了一种基于CRISPR技术的封闭式全自动核酸提取检测系统，可以避免检测时的核酸交叉污染，另外囊袋式生物芯片和控制检测器相对分离，可以快速更换检测样品，方便实际检测应用。

The invention discloses a closed full-automatic nucleic acid extraction and detection system based on a CRISPR technology. The closed full-automatic nucleic acid extraction and detection system comprises a bag type biological chip and a control detector, wherein the bag type biological chip comprises a nucleic acid extraction cavity, a No.1 cleaning liquid cavity, a No.2 cleaning liquid cavity, aNo.3 cleaning liquid cavity, an eluent cavity, a waste liquid cavity, an amplification cavity and a CRISPR detection cavity which are respectively used for storing various liquids required by nucleicacid extraction and detection, and the cavities are connected through micro-channels; and the control detector comprises a push rod controller, a controllable alternating electromagnet and a fluorescence detector, wherein the push rod controller is used for controlling a flow direction of liquid, the controllable alternating electromagnet is used for stirring and adsorbing magnetic beads, and thefluorescence detector is used for CRISPR luminescence detection of nucleic acid. According to the closed full-automatic nucleic acid extraction and detection system based on the CRISPR technology, nucleic acid cross contamination during detection can be avoided, in addition, the bag type biological chip and the control detector are relatively separated, a detection sample can be rapidly replaced, and practical detection application is facilitated.