Fibrogranular materials function as organizers to ensure the fidelity of multiciliary assembly

doi:10.1038/s41467-021-21506-8

	Fibrogranular materials function as organizers to ensure the fidelity of multiciliary assembly
	Zhao, Huijie 1,2; Chen, Qingxia1,3 ; Li, Fan 1,2; Cui, Lihong 4; Xie, Lele 1; Huang, Qiongping 1; Liang, Xin 4; Zhou, Jun 5; Yan, Xiumin 1; Zhu, Xueliang1,2,3,6
	2021-02-24
发表期刊	NATURE COMMUNICATIONS
ISSN	2041-1723
卷号	12 期号:1
DOI	10.1038/s41467-021-21506-8
摘要	Multicilia are delicate motile machineries, and how they are accurately assembled is poorly understood. Here, we show that fibrogranular materials (FGMs), large arrays of electron-dense granules specific to multiciliated cells, are essential for their ultrastructural fidelity. Pcm1 forms the granular units that further network into widespread FGMs, which are abundant in spherical FGM cores. FGM cores selectively concentrate multiple important centriole-related proteins as clients, including Cep131 that specifically decorates a foot region of ciliary central pair (CP) microtubules. FGMs also tightly contact deuterosome-procentriole complexes. Disruption of FGMs in mouse cells undergoing multiciliogenesis by Pcm1 RNAi markedly deregulates centriolar targeting of FGM clients, elongates CP-foot, and alters deuterosome size, number, and distribution. Although the multicilia are produced in correct numbers, they display abnormal ultrastructure and motility. Our results suggest that FGMs organize deuterosomes and centriole-related proteins to facilitate the faithful assembly of basal bodies and multiciliary axonemes. Multicilia are complex, and how they achieve accurate assembly is unclear. Here, the authors show that fibrogranular materials condense into spherical cores and function in multicilia formation by tightly associating with deuterosomes and concentrating specific proteins to promote proper assembly.
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收录类别	SCIE
语种	英语
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000626610200016
出版者	NATURE RESEARCH
原始文献类型	Article
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/126177
专题	生命科学与技术学院_博士生生命科学与技术学院_特聘教授组_朱学良组
通讯作者	Yan, Xiumin; Zhu, Xueliang
作者单位	1.Chinese Acad Sci, Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem & Cell Biol, State Key Lab Cell Biol, Shanghai 200031, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China; 4.Tsinghua Univ, Tsinghua Peking Joint Ctr Life Sci, Sch Life Sci, Beijing 100084, Peoples R China; 5.Shandong Normal Univ, Collaborat Innovat Ctr Cell Biol Univ Shandong, Coll Life Sci, Key Lab Anim Resistance Biol Shandong Prov,Inst B, Jinan 250014, Peoples R China; 6.Univ Chinese Acad Sci, Sch Life Sci, Hangzhou Inst Adv Study, Hangzhou 310024, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Zhao, Huijie,Chen, Qingxia,Li, Fan,et al. Fibrogranular materials function as organizers to ensure the fidelity of multiciliary assembly[J]. NATURE COMMUNICATIONS,2021,12(1).
APA	Zhao, Huijie.,Chen, Qingxia.,Li, Fan.,Cui, Lihong.,Xie, Lele.,...&Zhu, Xueliang.(2021).Fibrogranular materials function as organizers to ensure the fidelity of multiciliary assembly.NATURE COMMUNICATIONS,12(1).
MLA	Zhao, Huijie,et al."Fibrogranular materials function as organizers to ensure the fidelity of multiciliary assembly".NATURE COMMUNICATIONS 12.1(2021).