TRIM26 is a critical host factor for HCV replication and contributes to host tropism

doi:10.1126/sciadv.abd9732

	TRIM26 is a critical host factor for HCV replication and contributes to host tropism
	Liang, Yisha1,2,3 ; Zhang, Guigen 4,5; Li, Qiheng 4; Han, Lin1,2,3 ; Hu, Xiaoyou 1,3; Guo, Yu 4; Tao, Wanyin 1,7; Zhao, Xiaomin 6; Guo, Mingzhe1,2,3 ; Gan, Tianyu 1,3; Tong, Yimin 1; Xu, Yongfen 1; Zhou, Zhuo 4; Ding, Qiang 6; Wei, Wensheng 4; Zhong, Jin1,2,3
	2021-01
发表期刊	SCIENCE ADVANCES
ISSN	2375-2548
卷号	7 期号:2 页码:#VALUE!
DOI	10.1126/sciadv.abd9732
摘要	Hepatitis C virus (HCV) remains a major human pathogen that requires better understanding of virus-host interactions. In this study, we performed a genome-wide CRISPR-Cas9 screening and identified TRIM26, an E3 ligase, as a critical HCV host factor. Deficiency of TRIM26 specifically impairs HCV genome replication. Mechanistic studies showed that TRIM26 interacts with HCV-encoded NS5B protein and mediates its K27-linked ubiquitination at residue K51, and thus promotes the NS5B-NS5A interaction. Moreover, mouse TRIM26 does not support HCV replication because of its unique six-amino acid insert that prevents its interaction with NS5B. Ectopic expression of human TRIM26 in a mouse hepatoma cell line that has been reconstituted with other essential HCV host factors promotes HCV infection. In conclusion, we identified TRIM26 as a host factor for HCV replication and a new determinant of host tropism.These results shed light on HCV-host interactions and may facilitate the development of an HCV animal model.
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收录类别	SCI ; EI ; SCIE
语种	英语
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000606331400032
出版者	AMER ASSOC ADVANCEMENT SCIENCE
WOS关键词	HEPATITIS-C-VIRUS ; DEPENDENT RNA-POLYMERASE ; CRYSTAL-STRUCTURE ; INFECTION ; PROTEIN ; RECOGNITION ; BINDING ; CRISPR ; TBK1
原始文献类型	Article
引用统计	被引频次：24[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/125939
专题	生命科学与技术学院_博士生生命科学与技术学院_特聘教授组_钟劲组
通讯作者	Zhang, Guigen; Wei, Wensheng; Zhong, Jin
作者单位	1.Chinese Acad Sci, Inst Pasteur Shanghai, Unit Viral Hepatitis, CAS Key Lab Mol Virol & Immunol, Shanghai 200031, Peoples R China; 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China; 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 4.Peking Univ, Genome Editing Res Ctr,State Key Lab Prot & Plant, Biomed Pioneering Innovat Ctr BIOPIC,Sch Life Sci, Beijing Adv Innovat Ctr Genom ICG,Peking Tsinghua, Beijing 100871, Peoples R China; 5.Sun Yat Sen Univ, Zhongshan Sch Med, Inst Human Virol, Key Lab Trop Dis Control,Minist Educ, Guangzhou 510000, Guangdong, Peoples R China; 6.Tsinghua Univ, Ctr Infect Dis Res, Sch Med, Beijing 100084, Peoples R China; 7.Univ Sci & Technol China, Sch Basic Med Sci, Hefei Natl Lab Phys Sci Microscale, Div Life Sci & Med,CAS Key Lab Innate Immun & Chr, Hefei 230000, Peoples R China
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Liang, Yisha,Zhang, Guigen,Li, Qiheng,et al. TRIM26 is a critical host factor for HCV replication and contributes to host tropism[J]. SCIENCE ADVANCES,2021,7(2):#VALUE!.
APA	Liang, Yisha.,Zhang, Guigen.,Li, Qiheng.,Han, Lin.,Hu, Xiaoyou.,...&Zhong, Jin.(2021).TRIM26 is a critical host factor for HCV replication and contributes to host tropism.SCIENCE ADVANCES,7(2),#VALUE!.
MLA	Liang, Yisha,et al."TRIM26 is a critical host factor for HCV replication and contributes to host tropism".SCIENCE ADVANCES 7.2(2021):#VALUE!.