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| The cryo-EM structure of an ERAD protein channel formed by tetrameric human Derlin-1 | |
| 2021-03 | |
| 发表期刊 | SCIENCE ADVANCES
 |
| ISSN | 2375-2548 |
| 卷号 | 7期号:10页码:#VALUE! |
| DOI | 10.1126/sciadv.abe8591 |
| 摘要 | Endoplasmic reticulum-associated degradation (ERAD) is a process directing misfolded proteins from the ER lumen and membrane to the degradation machinery in the cytosol. A key step in ERAD is the translocation of ER proteins to the cytosol. Derlins are essential for protein translocation in ERAD, but the mechanism remains unclear. Here, we solved the structure of human Derlin-1 by cryo-electron microscopy. The structure shows that Derlin-1 forms a homotetramer that encircles a large tunnel traversing the ER membrane. The tunnel has a diameter of about 12 to 15 angstroms, large enough to allow an. helix to pass through. The structure also shows a lateral gate within the membrane, providing access of transmembrane proteins to the tunnel, and thus, human Derlin-1 forms a protein channel for translocation of misfolded proteins. Our structure is different from the monomeric yeast Derlin structure previously reported, which forms a semichannel with another protein. |
| URL | 查看原文 |
| 收录类别 | SCI ; SCIE |
| 语种 | 英语 |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| WOS记录号 | WOS:000625411900012 |
| 出版者 | AMER ASSOC ADVANCEMENT SCIENCE |
| 原始文献类型 | Article |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/125762 |
| 专题 | iHuman研究所_科学装置(X)_膜蛋白同步辐射线站 |
| 通讯作者 | Cao, Yu |
| 作者单位 | 1.Chinese Acad Sci, Univ Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem & Cell Biol, 333 Haike Rd, Shanghai 201210, Peoples R China; 2.Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Shanghai Inst Precis Med, Sch Med, 115 Jinzun Rd, Shanghai 200125, Peoples R China; 3.Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Orthopaed, Shanghai Key Lab Orthopaed Implant,Sch Med, Shanghai 200011, Peoples R China; 4.ShanghaiTech Univ, iHuman Inst, Shanghai 201210, Peoples R China |
| 推荐引用方式 GB/T 7714 | Rao, Bing,Li, Shaobai,Yao, Deqiang,et al. The cryo-EM structure of an ERAD protein channel formed by tetrameric human Derlin-1[J]. SCIENCE ADVANCES,2021,7(10):#VALUE!. |
| APA | Rao, Bing.,Li, Shaobai.,Yao, Deqiang.,Wang, Qian.,Xia, Ying.,...&Cao, Yu.(2021).The cryo-EM structure of an ERAD protein channel formed by tetrameric human Derlin-1.SCIENCE ADVANCES,7(10),#VALUE!. |
| MLA | Rao, Bing,et al."The cryo-EM structure of an ERAD protein channel formed by tetrameric human Derlin-1".SCIENCE ADVANCES 7.10(2021):#VALUE!. |
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