The Microfluidic Environment Reveals a Hidden Role of Self-Organizing Extracellular Matrix in Hepatic Commitment and Organoid Formation of hiPSCs

doi:10.1016/j.celrep.2020.108453

KMS > 免疫化学研究所 > 特聘教授组 > Nicola Elvassore组

	The Microfluidic Environment Reveals a Hidden Role of Self-Organizing Extracellular Matrix in Hepatic Commitment and Organoid Formation of hiPSCs
	Michielin, Federica 1; Giobbe, Giovanni G.1; Luni, Camilla2 ; Hu, Qianjiang2 ; Maroni, Ida 3,4; Orford, Michael R.1; Manfredi, Anna 5; Di Filippo, Lucio 6; David, Anna L.7; Cacchiarelli, Davide 5,8; De Coppi, Paolo 1,9; Eaton, Simon 1; Elvassore, Nicola1,2,3,4
	2020-12
发表期刊	CELL REPORTS (IF:7.5[JCR-2023],8.5[5-Year])
ISSN	2211-1247
卷号	33 期号:9
发表状态	已发表
DOI	10.1016/j.celrep.2020.108453
摘要	The specification of the hepatic identity during human liver development is strictly controlled by extrinsic signals, yet it is still not clear how cells respond to these exogenous signals by activating secretory cascades, which are extremely relevant, especially in 3D self-organizing systems. Here, we investigate how the proteins secreted by human pluripotent stem cells (hPSCs) in response to developmental exogenous signals affect the progression from endoderm to the hepatic lineage, including their competence to generate nascent hepatic organoids. By using microfluidic confined environment and stable isotope labeling with amino acids in cell culture-coupled mass spectrometry (SILAC-MS) quantitative proteomic analysis, we find high abundancy of extracellular matrix (ECM)-associated proteins. Hepatic progenitor cells either derived in microfluidics or exposed to exogenous ECM stimuli show a significantly higher potential of forming hepatic organoids that can be rapidly expanded for several passages and further differentiated into functional hepatocytes. These results prove an additional control over the efficiency of hepatic organoid formation and differentiation for downstream applications.
收录类别	SCI ; SCIE
语种	英语
资助项目	OAK Foundation Award[W1095/OCAY-14-191] ; UKRI MRC[MR/S02090X/1] ; Telethon Foundation Award[GGP15275] ; European Research Council[759154] ; ShanghaiTech University[F-0301-15-009] ; Natural Science Foundation of China[31601178]
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000595658100011
出版者	CELL PRESS
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/124903
专题	免疫化学研究所_特聘教授组_生物工程学实验室生命科学与技术学院_硕士生
通讯作者	Elvassore, Nicola
作者单位	1.UCL, Great Ormond St Inst Child Hlth, London WC1N 1EH, England 2.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies SIAIS, Shanghai 201210, Peoples R China 3.Univ Padua, Dept Ind Engn, I-35131 Padua, Italy 4.Venetian Inst Mol Med VIMM, I-35129 Padua, Italy 5.Armenise Harvard Lab Integrat Genom, Telethon Inst Genet & Med TIGEM, I-80078 Pozzuoli, Italy 6.Next Generat Diagnost Srl, I-80078 Pozzuoli, Italy 7.UCL, Elizabeth Garrett Anderson Inst Womens Hlth, London WC1E 6AU, England 8.Univ Naples Federico II, Dept Translat Med, I-80131 Naples, Italy 9.Great Ormond St Hosp Sick Children, Specialist Neonatal & Paediat Surg, London WC1N 3JH, England
通讯作者单位	免疫化学研究所
推荐引用方式 GB/T 7714	Michielin, Federica,Giobbe, Giovanni G.,Luni, Camilla,et al. The Microfluidic Environment Reveals a Hidden Role of Self-Organizing Extracellular Matrix in Hepatic Commitment and Organoid Formation of hiPSCs[J]. CELL REPORTS,2020,33(9).
APA	Michielin, Federica.,Giobbe, Giovanni G..,Luni, Camilla.,Hu, Qianjiang.,Maroni, Ida.,...&Elvassore, Nicola.(2020).The Microfluidic Environment Reveals a Hidden Role of Self-Organizing Extracellular Matrix in Hepatic Commitment and Organoid Formation of hiPSCs.CELL REPORTS,33(9).
MLA	Michielin, Federica,et al."The Microfluidic Environment Reveals a Hidden Role of Self-Organizing Extracellular Matrix in Hepatic Commitment and Organoid Formation of hiPSCs".CELL REPORTS 33.9(2020).