Characterization of a New M4 Metalloprotease With Collagen-Swelling Ability From MarineVibrio pomeroyiStrain 12613

doi:10.3389/fmicb.2020.01868

	Characterization of a New M4 Metalloprotease With Collagen-Swelling Ability From MarineVibrio pomeroyiStrain 12613
	Wang, Yan 1; Liu, Bai-Xue 1; Cheng, Jun-Hui 1; Su, Hai-Nan 1; Sun, He-Min 1; Li, Chun-Yang 2,3; Yang, Liuyan4,5 ; Shen, Qing-Tao4 ; Zhang, Yu-Zhong 1,2,3; Zhang, Xia 6; Chen, Xiu-Lan 1,3
	2020-08-07
发表期刊	FRONTIERS IN MICROBIOLOGY (IF:4.0[JCR-2023],5.1[5-Year])
ISSN	1664-302X
卷号	11
发表状态	已发表
DOI	10.3389/fmicb.2020.01868
摘要	The ocean harbors a variety of bacteria that contain huge protease resources and offer a great potential for industrial and biotechnological applications. Here, we isolated a protease-secreting bacteriumVibrio pomeroyistrain 12613 from Atlantic seawater and purified a protease VP9 from strain 12613. VP9 was identified as a metalloprotease of the M4 family. VP9 could hydrolyze casein and gelatin but not elastin and collagen. With gelatin as the substrate, VP9 showed the highest activity at 40 degrees C and pH 6.0-8.0. It was stable at temperatures of 50 degrees C and less and in the range of pH 5.0-11.0. VP9 also had good tolerance to NaCl, non-ionic detergents, and organic solvent methanol. Unlike other M4 metalloproteases, VP9 has distinct collagen-swelling ability, and its collagen-swelling effect was concentration dependent. The relative expansion volume of collagen increased by approximately eightfold after treatment with 10 mu M VP9 at 37 degrees C for 12 h. The collagen-swelling mechanism of VP9 on bovine-insoluble type I collagen was further studied. Atomic force microscopy observation and biochemical analyses showed that VP9 can degrade proteoglycans in collagen fibers, resulting in the release of collagen fibrils from collagen fibers and the swelling of the latter. In addition, VP9 can degrade glycoproteins, a non-collagenous constituent interacting with collagen in the skin. The characteristics of VP9, such as sufficient specificity toward proteoglycans and glycoproteins but no activity toward collagen, suggest its promising potential in the unhairing and fiber-opening processing in leather industry.
关键词	marine microbial protease the thermolysin family (M4) collagen swelling proteoglycans glycoproteins
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收录类别	SCI ; SCIE
语种	英语
资助项目	National Key R&D Program of China[2018YFC0310704][2018YFC1406504] ; National Science Foundation of China[31670038][31670063][31971535] ; AoShan Talents Cultivation Program - Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS14] ; Taishan Scholars Program of Shandong Province[tspd20181203]
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000563318500001
出版者	FRONTIERS MEDIA SA
WOS关键词	PSEUDOALTEROMONAS SP SM9913 ; DESEASIN MCP-01 ; THERMOLYSIN ; PROTEASE ; DOMAIN ; PURIFICATION ; HYDROLYSIS ; FIBRONECTIN ; ASPARTAME ; VIMELYSIN
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/123068
专题	iHuman研究所_PI研究组_沈庆涛组生命科学与技术学院_硕士生
通讯作者	Zhang, Xia; Chen, Xiu-Lan
作者单位	1.Shandong Univ, Marine Biotechnol Res Ctr, State Key Lab Microbial Technol, Qingdao, Peoples R China 2.Ocean Univ China, Coll Marine Life Sci, Frontiers Sci Ctr Deep Ocean Multispheres & Earth, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.ShanghaiTech Univ, Sch Life Sci & Technol, iHuman Inst, Shanghai, Peoples R China 5.Univ Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Shanghai, Peoples R China 6.Qingdao Vland Biotech Inc, Dept Mol Biol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yan,Liu, Bai-Xue,Cheng, Jun-Hui,et al. Characterization of a New M4 Metalloprotease With Collagen-Swelling Ability From MarineVibrio pomeroyiStrain 12613[J]. FRONTIERS IN MICROBIOLOGY,2020,11.
APA	Wang, Yan.,Liu, Bai-Xue.,Cheng, Jun-Hui.,Su, Hai-Nan.,Sun, He-Min.,...&Chen, Xiu-Lan.(2020).Characterization of a New M4 Metalloprotease With Collagen-Swelling Ability From MarineVibrio pomeroyiStrain 12613.FRONTIERS IN MICROBIOLOGY,11.
MLA	Wang, Yan,et al."Characterization of a New M4 Metalloprotease With Collagen-Swelling Ability From MarineVibrio pomeroyiStrain 12613".FRONTIERS IN MICROBIOLOGY 11(2020).