Cdc13 is predominant over Stn1 and Ten1 in preventing chromosome end fusions

doi:10.7554/eLife.53144

	Cdc13 is predominant over Stn1 and Ten1 in preventing chromosome end fusions
	Wu, Zhi-Jing 1,2; Liu, Jia-Cheng 1,2; Man, Xin 1,2; Gu, Xin 1,2; Li, Ting-Yi 1,2; Cai, Chen1,2,3 ; He, Ming-Hong 1,2; Shao, Yangyang 2,4; Lu, Ning 2,4; Xue, Xiaoli 2,4; Qin, Zhongjun 2,4; Zhou, Jin-Qiu1,2,3
	2020-08-05
发表期刊	ELIFE (IF:6.4[JCR-2023],7.2[5-Year])
ISSN	2050-084X
卷号	9
发表状态	已发表
DOI	10.7554/eLife.53144
摘要	Telomeres define the natural ends of eukaryotic chromosomes and are crucial for chromosomal stability. The budding yeast Cdc13, Stn1 and Ten1 proteins form a heterotrimeric complex, and the inactivation of any of its subunits leads to a uniformly lethal phenotype due to telomere deprotection. Although Cdc13, Stn1 and Ten1 seem to belong to an epistasis group, it remains unclear whether they function differently in telomere protection. Here, we employed the single-linear-chromosome yeast SY14, and surprisingly found that the deletion of CDC13 leads to telomere erosion and intrachromosome end-to-end fusion, which depends on Rad52 but not Yku. Interestingly, the emergence frequency of survivors in the SY14 cdc13 Delta mutant was similar to 29 fold higher than that in either the stn1 Delta or ten1 Delta mutant, demonstrating a predominant role of Cdc13 in inhibiting telomere fusion. Chromosomal fusion readily occurred in the telomerase-null SY14 strain, further verifying the default role of intact telomeres in inhibiting chromosome fusion.
收录类别	SCI ; SCIE
语种	英语
资助项目	National Key Research and Development Program of China[2016YFA0500701][31230040][18JC1420202][XDB19000000]
WOS研究方向	Life Sciences & Biomedicine - Other Topics
WOS类目	Biology
WOS记录号	WOS:000558756700001
出版者	ELIFE SCIENCES PUBLICATIONS LTD
WOS关键词	STRAND-BREAK REPAIR ; DNA-BINDING PROTEIN ; TELOMERE-TELOMERE RECOMBINATION ; SACCHAROMYCES-CEREVISIAE ; HOMOLOGOUS RECOMBINATION ; INDUCED REPLICATION ; CATALYTIC SUBUNIT ; LENGTH REGULATION ; POLYMERASE-ALPHA ; YEAST TELOMERES
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/122949
专题	生命科学与技术学院_博士生生命科学与技术学院_特聘教授组_周金秋组
通讯作者	Zhou, Jin-Qiu
作者单位	1.Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai, Peoples R China 2.Univ Chinese Acad Sci, Shanghai, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 4.Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, Shanghai Inst Plant Physiol & Ecol, Key Lab Synthet Biol, Shanghai, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Wu, Zhi-Jing,Liu, Jia-Cheng,Man, Xin,et al. Cdc13 is predominant over Stn1 and Ten1 in preventing chromosome end fusions[J]. ELIFE,2020,9.
APA	Wu, Zhi-Jing.,Liu, Jia-Cheng.,Man, Xin.,Gu, Xin.,Li, Ting-Yi.,...&Zhou, Jin-Qiu.(2020).Cdc13 is predominant over Stn1 and Ten1 in preventing chromosome end fusions.ELIFE,9.
MLA	Wu, Zhi-Jing,et al."Cdc13 is predominant over Stn1 and Ten1 in preventing chromosome end fusions".ELIFE 9(2020).