CRISPR-Cas12a-Assisted Genome Editing in Amycolatopsis mediterranei

doi:10.3389/fbioe.2020.00698

	CRISPR-Cas12a-Assisted Genome Editing in Amycolatopsis mediterranei
	Zhou, Yajuan1,2,3 ; Liu, Xinqiang 1,2; Wu, Jiacheng1,2,3 ; Zhao, Guoping 1,4; Wang, Jin 5
	2020-06-26
发表期刊	FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (IF:4.3[JCR-2023],5.1[5-Year])
ISSN	22964185
卷号	8
发表状态	已发表
DOI	10.3389/fbioe.2020.00698
摘要	Amycolatopsis mediterranei U32 is an industrial producer of rifamycin SV, whose derivatives have long been the first-line antimycobacterial drugs. In order to perform genetic modification in this important industrial strain, a lot of efforts have been made in the past decades and a homologous recombination-based method was successfully developed in our laboratory, which, however, requires the employment of an antibiotic resistance gene for positive selection and did not support convenient markerless gene deletion. Here in this study, the clustered regularly interspaced short palindromic repeat (CRISPR) system was employed to establish a genome editing system in A. mediterranei U32. Specifically, the Francisella tularensis subsp. novicida Cas12a (FnCas12a) gene was first integrated into the U32 genome to generate target-specific double-stranded DNA (dsDNA) breaks (DSBs) under the guidance of CRISPR RNAs (crRNAs). Then, the DSBs could be repaired by either the non-homologous DNA end-joining (NHEJ) system or the homology-directed repair (HDR) pathway, generating inaccurate or accurate mutations in target genes, respectively. Besides of A. mediterranei, the present work may also shed light on the development of CRISPR-assisted genome editing systems in other species of the Amycolatopsis genus. © Copyright © 2020 Zhou, Liu, Wu, Zhao and Wang.
关键词	Amycolatopsis mediterranei CRISPR-Cas12a genome editing NHEJ HDR
URL	查看原文
收录类别	SCI ; SCIE ; EI
语种	英语
资助项目	National Natural Science Foundation of China[31770057]
WOS研究方向	Biotechnology & Applied Microbiology ; Science & Technology - Other Topics
WOS类目	Biotechnology & Applied Microbiology ; Multidisciplinary Sciences
WOS记录号	WOS:000551740600001
出版者	Frontiers Media S.A.
EI入藏号	20202808930690
EI主题词	Biotechnology ; Genetic engineering
EI分类号	Bioengineering and Biology:461
WOS关键词	AMYCOLATOPSIS-MEDITERRANEI ; ESCHERICHIA-COLI ; RIFAMYCIN-B ; MECHANISM ; CRISPR/CAS ; CLASSIFICATION ; ENDONUCLEASE ; EVOLUTION ; SYSTEM ; REPAIR
原始文献类型	Journal article (JA)
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/122698
专题	生命科学与技术学院_硕士生
通讯作者	Wang, Jin
作者单位	1.CAS Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China 2.University of Chinese Academy of Sciences, Beijing, China 3.School of Life Sciences and Technology, Shanghai Tech University, Shanghai, China 4.Department of Microbiology and Li, Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong 5.College of Life Sciences, Shanghai Normal University, Shanghai, China
第一作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Zhou, Yajuan,Liu, Xinqiang,Wu, Jiacheng,et al. CRISPR-Cas12a-Assisted Genome Editing in Amycolatopsis mediterranei[J]. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,2020,8.
APA	Zhou, Yajuan,Liu, Xinqiang,Wu, Jiacheng,Zhao, Guoping,&Wang, Jin.(2020).CRISPR-Cas12a-Assisted Genome Editing in Amycolatopsis mediterranei.FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,8.
MLA	Zhou, Yajuan,et al."CRISPR-Cas12a-Assisted Genome Editing in Amycolatopsis mediterranei".FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 8(2020).