Novel Variants in GDF9 Gene Affect Promoter Activity and Litter Size in Mongolia Sheep

doi:10.3390/genes11040375

	Novel Variants in GDF9 Gene Affect Promoter Activity and Litter Size in Mongolia Sheep
	Tong, Bin 1; Wang, Jiapeng 1; Cheng, Zixuan 1; Liu, Jiasen 2; Wu, Yiran3 ; Li, Yunhua 2; Bai, Chunling 1; Zhao, Suwen3 ; Yu, Haiquan 1; Li, Guangpeng 1
	2020-04
发表期刊	GENES (IF:2.8[JCR-2023],3.3[5-Year])
EISSN	2073-4425
卷号	11 期号:4
发表状态	已发表
DOI	10.3390/genes11040375
摘要	Litter size is an economically important trait in sheep breeding. The objectives of this study were as follows: (1) to ascertain if any of the 19 known variants in the BMPRIB, BMP15, and GDF9 genes are present and associated with the litter size of Mongolia sheep; (2) to identify novel variants in GDF9 and perform association analysis; and (3) to validate the effects of these GDF9 promoter variants on the activity of the gene. The results of the 19 known variants showed that the FecB(B) affected the litter size of Mongolia sheep (p < 0.001). The association analysis results of novel variants showed that the g.46544883A>G (GenBank accession: NC_040256, the same below) in the 3' untranslated region (3' UTR), the c.1040T>C (Phe347Ser) in the exon 2, and the g.46547859C>T SNP in the promotor of GDF9 were significantly associated with litter size of Mongolia ewes (p < 0.01, p < 0.05, and p < 0.001, respectively). In addition, the GDF9 promoter activity analysis showed that the C allele at the -332 position (g.46547859C>T) could decrease luciferase activity compared with the T allele (p < 0.01). Our findings may facilitate effective marker-assisted selection to increase litter size in Mongolia sheep populations, as well as bring new insights into GDF9 expression.
关键词	FecB(B) GDF9 litter size Mongolia sheep promoter activity molecular markers
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收录类别	SCI ; SCIE
语种	英语
资助项目	Inner Mongolia Science & Technology Plan[20140171][201803005][2019ZD08] ; "Grassland Meritocrat" Scholars Program of the Inner Mongolia Autonomous Region[CYYC6068] ; High-level Talents Import Program of Inner Mongolia University[21400-5165112] ; High Level Overseas Talents Project ([2018]) from the Ministry of Human Resources and Social Security of China[190]
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:000537224600119
出版者	MDPI
WOS关键词	INCREASED OVULATION RATE ; FOLLICULAR DEVELOPMENT ; DOMESTIC SHEEP ; FECB GENE ; BMPR-IB ; MUTATION ; GROWTH ; POLYMORPHISM ; PROLIFICACY ; STERILITY
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/121625
专题	iHuman研究所_PI研究组_赵素文组生命科学与技术学院
通讯作者	Tong, Bin
作者单位	1.Inner Mongolia Univ, Res Ctr Lab Anim Sci, Sch Life Sci, State Key Lab Reprod Regulat & Breeding Grassland, Hohhot 010070, Peoples R China 2.Inner Mongolia Acad Agr & Anim Husb Sci, Inst Anim Sci, Hohhot 010018, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, iHuman Inst, Shanghai 201210, Peoples R China
推荐引用方式 GB/T 7714	Tong, Bin,Wang, Jiapeng,Cheng, Zixuan,et al. Novel Variants in GDF9 Gene Affect Promoter Activity and Litter Size in Mongolia Sheep[J]. GENES,2020,11(4).
APA	Tong, Bin.,Wang, Jiapeng.,Cheng, Zixuan.,Liu, Jiasen.,Wu, Yiran.,...&Li, Guangpeng.(2020).Novel Variants in GDF9 Gene Affect Promoter Activity and Litter Size in Mongolia Sheep.GENES,11(4).
MLA	Tong, Bin,et al."Novel Variants in GDF9 Gene Affect Promoter Activity and Litter Size in Mongolia Sheep".GENES 11.4(2020).