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Improvement of polyhydroxybutyrate production by deletion of csrA in Escherichia coli | |
2020-07 | |
发表期刊 | ELECTRONIC JOURNAL OF BIOTECHNOLOGY (IF:2.3[JCR-2023],2.5[5-Year]) |
ISSN | 07173458 |
卷号 | 46页码:8-13 |
发表状态 | 已发表 |
DOI | 10.1016/j.ejbt.2020.04.005 |
摘要 | Background: Poly-3-hydroxybutyrate (PHB) can be efficiently produced in recombinant Escherichia coli by the overexpression of an operon (NphaCAB) encoding PHB synthetase. Strain improvement is considered to be one of critical factors to lower the production cost of PHB in recombinant system. In this study, one of key regulators that affect the cell growth and PHB content was confirmed and analyzed. Result: S17-3, a mutant E. coli strain derived from S17-1, was found to be able to achieve high cell density when expressing NphaCAB with the plasmid pBhya-CAB. Whole genome sequencing of S17-3 revealed genetic alternations on the upstream regions of csrA, encoding a global regulator cross-talking between stress response, catabolite repression and other metabolic activities. Deletion of csrA or expression of mutant csrA resulted in improved cell density and PHB content. Conclusion: The impact of gene deletion of csrA was determined, dysfunction of the regulators improved the cell density of recombinant E. coli and PHB production, however, the detail mechanism needs to be further clarified. How to cite: Wu H, Li S, Ji M, et al. Improvement of polyhydroxybutyrate production by deletion of csrA in Escherichia coli. Electron J Biotechnol 2020;46. https://doi.org/10.1016/j.ejbt.2020.04.005. |
收录类别 | SCI ; SCIE ; EI |
语种 | 英语 |
资助项目 | Scientific Research Foundation for Returned Scholars of Ministry of Education[] ; National Natural Science Foundation of China[] |
WOS记录号 | WOS:000557952300002 |
出版者 | Pontificia Universidad Catolica de Valparaiso |
EI入藏号 | 20202208761943 |
EI主题词 | Cell proliferation ; Cells ; Cytology ; Encoding (symbols) ; Signal encoding |
EI分类号 | Biological Materials and Tissue Engineering:461.2 ; Biology:461.9 ; Information Theory and Signal Processing:716.1 ; Data Processing and Image Processing:723.2 |
原始文献类型 | Journal article (JA) |
引用统计 | 正在获取...
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文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/121579 |
专题 | 生命科学与技术学院_博士生 生命科学与技术学院_特聘教授组_孙俊松组 生命科学与技术学院_特聘教授组_史吉平组 |
通讯作者 | Sun, Junsong |
作者单位 | 1.Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong; Shanghai; 201210, China 2.School of Life Science and Technology, Shanghai Tech University, Shanghai; 201210, China 3.University of Chinese Academy of Sciences, Beijing; 100049, China 4.Facultad de Química y Biología, Departamento de Biología, Universidad de Santiago de Chile, Santiago, Chile 5.Fundación Científica y Cultural Biociencia, Santiago, Chile |
第一作者单位 | 生命科学与技术学院 |
通讯作者单位 | 生命科学与技术学院 |
推荐引用方式 GB/T 7714 | Wu, Haiying,Li, Sijie,Ji, Minghua,et al. Improvement of polyhydroxybutyrate production by deletion of csrA in Escherichia coli[J]. ELECTRONIC JOURNAL OF BIOTECHNOLOGY,2020,46:8-13. |
APA | Wu, Haiying.,Li, Sijie.,Ji, Minghua.,Chen, Qiao.,Shi, Jiping.,...&Sun, Junsong.(2020).Improvement of polyhydroxybutyrate production by deletion of csrA in Escherichia coli.ELECTRONIC JOURNAL OF BIOTECHNOLOGY,46,8-13. |
MLA | Wu, Haiying,et al."Improvement of polyhydroxybutyrate production by deletion of csrA in Escherichia coli".ELECTRONIC JOURNAL OF BIOTECHNOLOGY 46(2020):8-13. |
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