Improvement of polyhydroxybutyrate production by deletion of csrA in Escherichia coli
2020-07
发表期刊ELECTRONIC JOURNAL OF BIOTECHNOLOGY (IF:2.3[JCR-2023],2.5[5-Year])
ISSN07173458
卷号46页码:8-13
发表状态已发表
DOI10.1016/j.ejbt.2020.04.005
摘要

Background: Poly-3-hydroxybutyrate (PHB) can be efficiently produced in recombinant Escherichia coli by the overexpression of an operon (NphaCAB) encoding PHB synthetase. Strain improvement is considered to be one of critical factors to lower the production cost of PHB in recombinant system. In this study, one of key regulators that affect the cell growth and PHB content was confirmed and analyzed. Result: S17-3, a mutant E. coli strain derived from S17-1, was found to be able to achieve high cell density when expressing NphaCAB with the plasmid pBhya-CAB. Whole genome sequencing of S17-3 revealed genetic alternations on the upstream regions of csrA, encoding a global regulator cross-talking between stress response, catabolite repression and other metabolic activities. Deletion of csrA or expression of mutant csrA resulted in improved cell density and PHB content. Conclusion: The impact of gene deletion of csrA was determined, dysfunction of the regulators improved the cell density of recombinant E. coli and PHB production, however, the detail mechanism needs to be further clarified. How to cite: Wu H, Li S, Ji M, et al. Improvement of polyhydroxybutyrate production by deletion of csrA in Escherichia coli. Electron J Biotechnol 2020;46. https://doi.org/10.1016/j.ejbt.2020.04.005.
© 2020

收录类别SCI ; SCIE ; EI
语种英语
资助项目Scientific Research Foundation for Returned Scholars of Ministry of Education[] ; National Natural Science Foundation of China[]
WOS记录号WOS:000557952300002
出版者Pontificia Universidad Catolica de Valparaiso
EI入藏号20202208761943
EI主题词Cell proliferation ; Cells ; Cytology ; Encoding (symbols) ; Signal encoding
EI分类号Biological Materials and Tissue Engineering:461.2 ; Biology:461.9 ; Information Theory and Signal Processing:716.1 ; Data Processing and Image Processing:723.2
原始文献类型Journal article (JA)
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文献类型期刊论文
条目标识符https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/121579
专题生命科学与技术学院_博士生
生命科学与技术学院_特聘教授组_孙俊松组
生命科学与技术学院_特聘教授组_史吉平组
通讯作者Sun, Junsong
作者单位
1.Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong; Shanghai; 201210, China
2.School of Life Science and Technology, Shanghai Tech University, Shanghai; 201210, China
3.University of Chinese Academy of Sciences, Beijing; 100049, China
4.Facultad de Química y Biología, Departamento de Biología, Universidad de Santiago de Chile, Santiago, Chile
5.Fundación Científica y Cultural Biociencia, Santiago, Chile
第一作者单位生命科学与技术学院
通讯作者单位生命科学与技术学院
推荐引用方式
GB/T 7714
Wu, Haiying,Li, Sijie,Ji, Minghua,et al. Improvement of polyhydroxybutyrate production by deletion of csrA in Escherichia coli[J]. ELECTRONIC JOURNAL OF BIOTECHNOLOGY,2020,46:8-13.
APA Wu, Haiying.,Li, Sijie.,Ji, Minghua.,Chen, Qiao.,Shi, Jiping.,...&Sun, Junsong.(2020).Improvement of polyhydroxybutyrate production by deletion of csrA in Escherichia coli.ELECTRONIC JOURNAL OF BIOTECHNOLOGY,46,8-13.
MLA Wu, Haiying,et al."Improvement of polyhydroxybutyrate production by deletion of csrA in Escherichia coli".ELECTRONIC JOURNAL OF BIOTECHNOLOGY 46(2020):8-13.
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