Human colorectal cancer-specific CCAT1-L lncRNA regulates long-range chromatin interactions at the MYC locus

doi:10.1038/cr.2014.35

	Human colorectal cancer-specific CCAT1-L lncRNA regulates long-range chromatin interactions at the MYC locus
	Xiang, Jian-Feng ; Yin, Qing-Fei ; Chen, Tian ; Zhang, Yang ; Zhang, Xiao-Ou ; Wu, Zheng ; Zhang, Shaofeng ; Wang, Hai-Bin ; Ge, Junhui ; Lu, Xuhua ; Yang, Li ; Chen, Ling-Ling
	2014
发表期刊	CELL RESEARCH
ISSN	1001-0602
EISSN	1748-7838
卷号	24 期号:5 页码:513-531
发表状态	已发表
DOI	10.1038/cr.2014.35
摘要	The human 8q24 gene desert contains multiple enhancers that form tissue-specific long-range chromatin loops with the MYC oncogene, but how chromatin looping at the MYC locus is regulated remains poorly understood. Here we demonstrate that a long noncoding RNA (lncRNA), CCAT1-L, is transcribed specifically in human colorectal cancers from a locus 515 kb upstream of MYC. This lncRNA plays a role in MYC transcriptional regulation and promotes long-range chromatin looping. Importantly, the CCAT1-L locus is located within a strong super-enhancer and is spatially close to MYC. Knockdown of CCAT1-L reduced long-range interactions between the MYC promoter and its enhancers. In addition, CCAT1-L interacts with CTCF and modulates chromatin conformation at these loop regions. These results reveal an important role of a previously unannotated lncRNA in gene regulation at the MYC locus.
关键词	CCAT1-L lncRNA super-enhancer chromatin looping MYC CTCF
URL	查看原文
收录类别	SCI
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000335914700002
WOS关键词	NONCODING RNA ; COLON-CANCER ; TRANSCRIPTION FACTORS ; SUPER-ENHANCERS ; LINEAGE COMMITMENT ; GENE-EXPRESSION ; CELL IDENTITY ; SNP RS6983267 ; HUMAN GENOME ; CTCF
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/120214
专题	个人在本单位外知识产出
通讯作者	Chen, Ling-Ling
作者单位	1.Chinese Acad Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, CAS MPG Partner Inst Computat Biol, Key Lab Computat Biol, Shanghai 200031, Peoples R China 3.Second Mil Med Univ, Changzheng Hosp, Shanghai 200003, Peoples R China
推荐引用方式 GB/T 7714	Xiang, Jian-Feng,Yin, Qing-Fei,Chen, Tian,et al. Human colorectal cancer-specific CCAT1-L lncRNA regulates long-range chromatin interactions at the MYC locus[J]. CELL RESEARCH,2014,24(5):513-531.
APA	Xiang, Jian-Feng.,Yin, Qing-Fei.,Chen, Tian.,Zhang, Yang.,Zhang, Xiao-Ou.,...&Chen, Ling-Ling.(2014).Human colorectal cancer-specific CCAT1-L lncRNA regulates long-range chromatin interactions at the MYC locus.CELL RESEARCH,24(5),513-531.
MLA	Xiang, Jian-Feng,et al."Human colorectal cancer-specific CCAT1-L lncRNA regulates long-range chromatin interactions at the MYC locus".CELL RESEARCH 24.5(2014):513-531.