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Sexual Fate Change of XX Germ Cells Caused by the Deletion of SMAD4 and STRA8 Independent of Somatic Sex Reprogramming | |
2016 | |
发表期刊 | PLOS BIOLOGY |
ISSN | 1545-7885 |
卷号 | 14期号:9 |
发表状态 | 已发表 |
DOI | 10.1371/journal.pbio.1002553 |
摘要 | The differential programming of sperm and eggs in gonads is a fundamental topic in reproductive biology. Although the sexual fate of germ cells is believed to be determined by signaling factors from sexually differentiated somatic cells in fetal gonads, the molecular mechanism that determines germ cell fate is poorly understood. Herein, we show that mothers against decapentaplegic homolog 4 (SMAD4) in germ cells is required for female-type differentiation. Germ cells in Smad4-deficient ovaries respond to retinoic acid signaling but fail to undergo meiotic prophase I, which coincides with the weaker expression of genes required for follicular formation, indicating that SMAD4 signaling is essential for oocyte differentiation and meiotic progression. Intriguingly, germline-specific deletion of Smad4 in Stra8-null female germ cells resulted in the up-regulation of genes required for male gonocyte differentiation, including Nanos2 and PLZF, suggesting the initiation of male-type differentiation in ovaries. Moreover, our transcriptome analyses of mutant ovaries revealed that the sex change phenotype is achieved without global gene expression changes in somatic cells. Our results demonstrate that SMAD4 and STRA8 are essential factors that regulate the female fate of germ cells. |
URL | 查看原文 |
收录类别 | SCI |
WOS研究方向 | Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics |
WOS类目 | Biochemistry & Molecular Biology ; Biology |
WOS记录号 | WOS:000386128900010 |
WOS关键词 | MOUSE TESTIS DIFFERENTIATION ; CHROMOSOME SYNAPSIS ; RETINOIC ACID ; KNOCKOUT MICE ; SUPPRESSES MEIOSIS ; MEIOTIC INITIATION ; BETA-CATENIN ; EXPRESSION ; NANOS2 ; GENE |
原始文献类型 | Article |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/119952 |
专题 | 个人在本单位外知识产出 |
通讯作者 | Saga, Yumiko |
作者单位 | 1.Sokendai, Dept Genet, Mishima, Shizuoka, Japan 2.Natl Inst Genet, Div Mammalian Dev, Mishima, Shizuoka, Japan 3.Univ Macau, Fac Hlth Sci, Macau, Macau, Peoples R China 4.Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Tokyo, Japan |
推荐引用方式 GB/T 7714 | Wu, Quan,Fukuda, Kurumi,Kato, Yuzuru,et al. Sexual Fate Change of XX Germ Cells Caused by the Deletion of SMAD4 and STRA8 Independent of Somatic Sex Reprogramming[J]. PLOS BIOLOGY,2016,14(9). |
APA | Wu, Quan,Fukuda, Kurumi,Kato, Yuzuru,Zhou, Zhi,Deng, Chu-Xia,&Saga, Yumiko.(2016).Sexual Fate Change of XX Germ Cells Caused by the Deletion of SMAD4 and STRA8 Independent of Somatic Sex Reprogramming.PLOS BIOLOGY,14(9). |
MLA | Wu, Quan,et al."Sexual Fate Change of XX Germ Cells Caused by the Deletion of SMAD4 and STRA8 Independent of Somatic Sex Reprogramming".PLOS BIOLOGY 14.9(2016). |
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