New insights into the structural basis of DNA recognition by HINa and HINb domains of IFI16
Ni, Xiangmin1,2; Ru, Heng2; Ma, Feng3; Zhao, Lixia2; Shaw, Neil2; Feng, Yingang4,5; Ding, Wei2; Gong, Weibin2; Wang, Qiaofeng1; Ouyang, Songying2
2016-02
发表期刊JOURNAL OF MOLECULAR CELL BIOLOGY
ISSN1674-2788
卷号8期号:1页码:51-61
发表状态已发表
DOI10.1093/jmcb/mjv053
摘要Interferon gamma-inducible protein 16 (IFI16) senses DNA in the cytoplasm and the nucleus by using two tandem hematopoietic interferon-inducible nuclear (HIN) domains, HINa and HINb, through the cooperative assembly of IFI16 filaments on double-stranded DNA (dsDNA). The role of HINa in sensing DNA is not clearly understood. Here, we describe the crystal structure of the HINa domain in complex with DNA at 2.55 resolution and provide the first insight into the mode of DNA binding by the HINa domain. The structure reveals the presence of two oligosaccharide/nucleotide-binding (OB) folds with a unique DNA-binding surface. HINa uses loop L-45 of the canonical OB2 fold to bind to the DNA backbone. The dsDNA is recognized as two single strands of DNA. Interestingly, deletion of HINb compromises the ability of IFI16 to induce IFN-beta, while HINa mutants impaired in DNA binding enhance the production of IFN-beta. These results shed light on the roles of IFI16 HIN domains in DNA recognition and innate immune responses.
关键词interferon gamma-inducible protein 16 (IFI16) hematopoietic interferon-inducible nuclear (HIN) domain DNA recognition innate immune responses
收录类别SCI
语种英语
资助项目Beijing Nova Program[Z141102001814020]
WOS研究方向Cell Biology
WOS类目Cell Biology
WOS记录号WOS:000372615600006
出版者OXFORD UNIV PRESS
WOS关键词CYCLIC GMP-AMP ; CYTOSOLIC DNA ; PROVIDES INSIGHTS ; I INTERFERON ; PROTEIN ; SENSOR ; INFLAMMASOME ; ACTIVATION ; BINDING ; RNA
原始文献类型Article
引用统计
文献类型期刊论文
条目标识符https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1063
专题iHuman研究所
iHuman研究所_PI研究组_刘志杰组
通讯作者Ouyang, Songying; Cheng, Genhong; Liu, Zhi-Jie
作者单位1.Kunming Med Univ, Inst Mol & Clin Med, Kunming 650500, Peoples R China
2.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China
3.Univ Calif Los Angeles, Dept Microbiol Immunol & Mol Genet, Los Angeles, CA 90095 USA
4.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao Engn Lab Single Cell Oil, Qingdao 266101, Peoples R China
5.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Peoples R China
6.ShanghaiTech Univ, IHuman Inst, Shanghai 201210, Peoples R China
通讯作者单位iHuman研究所
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Ni, Xiangmin,Ru, Heng,Ma, Feng,et al. New insights into the structural basis of DNA recognition by HINa and HINb domains of IFI16[J]. JOURNAL OF MOLECULAR CELL BIOLOGY,2016,8(1):51-61.
APA Ni, Xiangmin.,Ru, Heng.,Ma, Feng.,Zhao, Lixia.,Shaw, Neil.,...&Liu, Zhi-Jie.(2016).New insights into the structural basis of DNA recognition by HINa and HINb domains of IFI16.JOURNAL OF MOLECULAR CELL BIOLOGY,8(1),51-61.
MLA Ni, Xiangmin,et al."New insights into the structural basis of DNA recognition by HINa and HINb domains of IFI16".JOURNAL OF MOLECULAR CELL BIOLOGY 8.1(2016):51-61.
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