Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture

doi:10.1038/s41467-019-13605-4

KMS > 免疫化学研究所 > 特聘教授组 > Nicola Elvassore组

	Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture
	Giobbe, Giovanni Giuseppe 1; Crowley, Claire 1; Luni, Camilla2 ; Campinoti, Sara 1,3; Khedr, Moustafa 1; Kretzschmar, Kai 4,5; De Santis, Martina Maria 1; Zambaiti, Elisa 1; Michielin, Federica 1; Meran, Laween 1,6; Hu, Qianjiang2 ; van Son, Gijs 4,5; Urbani, Luca 1; Manfredi, Anna 7; Giomo, Monica 8,9; Eaton, Simon 1; Cacchiarelli, Davide 7; Li, Vivian S. W.6; Clevers, Hans 4,5,10; Bonfanti, Paola 1,3; Elvassore, Nicola1,2,8,9 ; De Coppi, Paolo 1,11
	2019-12-11
发表期刊	NATURE COMMUNICATIONS (IF:14.7[JCR-2023],16.1[5-Year])
ISSN	2041-1723
卷号	10
发表状态	已发表
DOI	10.1038/s41467-019-13605-4
摘要	Organoids have extensive therapeutic potential and are increasingly opening up new avenues within regenerative medicine. However, their clinical application is greatly limited by the lack of effective GMP-compliant systems for organoid expansion in culture. Here, we envisage that the use of extracellular matrix (ECM) hydrogels derived from decellularized tissues (DT) can provide an environment capable of directing cell growth. These gels possess the biochemical signature of tissue-specific ECM and have the potential for clinical translation. Gels from decellularized porcine small intestine (SI) mucosa/submucosa enable formation and growth of endoderm-derived human organoids, such as gastric, hepatic, pancreatic, and SI. ECM gels can be used as a tool for direct human organoid derivation, for cell growth with a stable transcriptomic signature, and for in vivo organoid delivery. The development of these ECM-derived hydrogels opens up the potential for human organoids to be used clinically.
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收录类别	SCI ; SCIE
语种	英语
资助项目	[F-0301-15-009]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000502104400001
出版者	NATURE PUBLISHING GROUP
WOS关键词	SMALL-INTESTINAL SUBMUCOSA ; LONG-TERM EXPANSION ; STEM-CELLS ; IN-VITRO ; EXPRESSION ; COLON ; IDENTIFICATION ; ARCHITECTURE ; EPITHELIUM ; GROWTH
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/104869
专题	免疫化学研究所_特聘教授组_生物工程学实验室生命科学与技术学院_硕士生
通讯作者	Elvassore, Nicola; De Coppi, Paolo
作者单位	1.UCL, GOS Inst Child Hlth, Stem Cell & Regenerat Med Sect, London, England 2.ShanghaiTech Univ, SIAIS, Shanghai, Peoples R China 3.Francis Crick Inst, Epithelial Stem Cell Biol & Regenerat Med Lab, London, England 4.Royal Netherlands Acad Arts & Sci KNAW, Hubrecht Inst, Oncode Inst, Utrecht, Netherlands 5.Univ Med Ctr UMC Utrecht, Utrecht, Netherlands 6.Francis Crick Inst, Stem Cell & Canc Biol Lab, London, England 7.Telethon Inst Genet & Med TIGEM, Pozzuoli, Italy 8.Univ Padua, Veneto Inst Mol Med, Padua, Italy 9.Univ Padua, Dept Ind Engn, Padua, Italy 10.Princess Maxima Ctr PMC Pediat Oncol, Utrecht, Netherlands 11.Great Ormond St Hosp Sick Children, Specialist Neonatal & Paediat Surg Unit, London, England
通讯作者单位	上海科技大学
推荐引用方式 GB/T 7714	Giobbe, Giovanni Giuseppe,Crowley, Claire,Luni, Camilla,et al. Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture[J]. NATURE COMMUNICATIONS,2019,10.
APA	Giobbe, Giovanni Giuseppe.,Crowley, Claire.,Luni, Camilla.,Campinoti, Sara.,Khedr, Moustafa.,...&De Coppi, Paolo.(2019).Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture.NATURE COMMUNICATIONS,10.
MLA	Giobbe, Giovanni Giuseppe,et al."Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture".NATURE COMMUNICATIONS 10(2019).