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ShanghaiTech University Knowledge Management System
Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture | |
Giobbe, Giovanni Giuseppe1; Crowley, Claire1; Luni, Camilla2 ![]() ![]() ![]() | |
2019-12-11 | |
发表期刊 | NATURE COMMUNICATIONS (IF:14.7[JCR-2023],16.1[5-Year]) |
ISSN | 2041-1723 |
卷号 | 10 |
发表状态 | 已发表 |
DOI | 10.1038/s41467-019-13605-4 |
摘要 | Organoids have extensive therapeutic potential and are increasingly opening up new avenues within regenerative medicine. However, their clinical application is greatly limited by the lack of effective GMP-compliant systems for organoid expansion in culture. Here, we envisage that the use of extracellular matrix (ECM) hydrogels derived from decellularized tissues (DT) can provide an environment capable of directing cell growth. These gels possess the biochemical signature of tissue-specific ECM and have the potential for clinical translation. Gels from decellularized porcine small intestine (SI) mucosa/submucosa enable formation and growth of endoderm-derived human organoids, such as gastric, hepatic, pancreatic, and SI. ECM gels can be used as a tool for direct human organoid derivation, for cell growth with a stable transcriptomic signature, and for in vivo organoid delivery. The development of these ECM-derived hydrogels opens up the potential for human organoids to be used clinically. |
URL | 查看原文 |
收录类别 | SCI ; SCIE |
语种 | 英语 |
资助项目 | [F-0301-15-009] |
WOS研究方向 | Science & Technology - Other Topics |
WOS类目 | Multidisciplinary Sciences |
WOS记录号 | WOS:000502104400001 |
出版者 | NATURE PUBLISHING GROUP |
WOS关键词 | SMALL-INTESTINAL SUBMUCOSA ; LONG-TERM EXPANSION ; STEM-CELLS ; IN-VITRO ; EXPRESSION ; COLON ; IDENTIFICATION ; ARCHITECTURE ; EPITHELIUM ; GROWTH |
原始文献类型 | Article |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/104869 |
专题 | 免疫化学研究所_特聘教授组_生物工程学实验室 生命科学与技术学院_硕士生 |
通讯作者 | Elvassore, Nicola; De Coppi, Paolo |
作者单位 | 1.UCL, GOS Inst Child Hlth, Stem Cell & Regenerat Med Sect, London, England 2.ShanghaiTech Univ, SIAIS, Shanghai, Peoples R China 3.Francis Crick Inst, Epithelial Stem Cell Biol & Regenerat Med Lab, London, England 4.Royal Netherlands Acad Arts & Sci KNAW, Hubrecht Inst, Oncode Inst, Utrecht, Netherlands 5.Univ Med Ctr UMC Utrecht, Utrecht, Netherlands 6.Francis Crick Inst, Stem Cell & Canc Biol Lab, London, England 7.Telethon Inst Genet & Med TIGEM, Pozzuoli, Italy 8.Univ Padua, Veneto Inst Mol Med, Padua, Italy 9.Univ Padua, Dept Ind Engn, Padua, Italy 10.Princess Maxima Ctr PMC Pediat Oncol, Utrecht, Netherlands 11.Great Ormond St Hosp Sick Children, Specialist Neonatal & Paediat Surg Unit, London, England |
通讯作者单位 | 上海科技大学 |
推荐引用方式 GB/T 7714 | Giobbe, Giovanni Giuseppe,Crowley, Claire,Luni, Camilla,et al. Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Giobbe, Giovanni Giuseppe.,Crowley, Claire.,Luni, Camilla.,Campinoti, Sara.,Khedr, Moustafa.,...&De Coppi, Paolo.(2019).Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture.NATURE COMMUNICATIONS,10. |
MLA | Giobbe, Giovanni Giuseppe,et al."Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture".NATURE COMMUNICATIONS 10(2019). |
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