Nascent Pre-rRNA Sorting via Phase Separation Drives the Assembly of Dense Fibrillar Components in the Human Nucleolus

doi:10.1016/j.molcel.2019.08.014

	Nascent Pre-rRNA Sorting via Phase Separation Drives the Assembly of Dense Fibrillar Components in the Human Nucleolus
	Yao, Run-Wen 1; Xu, Guang 1; Wang, Ying 2; Shan, Lin 1; Luan, Peng-Fei 1; Wang, Yang 1; Wu, Man 1; Yang, Liang-Zhong 1; Xing, Yu-Hang 1,4,5; Yang, Li2,3 ; Chen, Ling-Ling1,3
	2019-12-05
发表期刊	MOLECULAR CELL
ISSN	1097-2765
EISSN	1097-4164
卷号	76 期号:5 页码:767-+
发表状态	已发表
DOI	10.1016/j.molcel.2019.08.014
摘要	Fibrillar centers (FCs) and dense fibrillar components (DFCs) are essential morphologically distinct sub-regions of mammalian cell nucleoli for rDNA transcription and pre-rRNA processing. Here, we report that a human nucleolus consists of several dozen FC/DFC units, each containing 2-3 transcriptionally active rDNAs at the FC/DFC border. Pre-rRNA processing factors, such as fibrillarin (FBL), form 18-24 clusters that further assemble into the DFC surrounding the FC. Mechanistically, the 5' end of nascent 47S pre-rRNA binds co-transcriptionally to the RNA-binding domain of FBL. FBL diffuses to the DFC, where local self-association via its glycine- and arginine-rich (GAR) domain forms phase-separated clusters to immobilize FBL-interacting pre-rRNA, thus promoting directional traffic of nascent pre-rRNA while facilitating pre-rRNA processing and DFC formation. These results unveil FC/DFC ultrastructures in nucleoli and suggest a conceptual framework for considering nascent RNA sorting using multivalent interactions of their binding proteins.
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收录类别	SCI ; SCIE
语种	英语
资助项目	HHMI[55008728]
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Cell Biology
WOS记录号	WOS:000500937100009
出版者	CELL PRESS
WOS关键词	POLYMERASE-I TRANSCRIPTION ; QUANTITATIVE-ANALYSIS ; ORGANIZER REGIONS ; SSU PROCESSOME ; BOX C/D ; BINDING ; HETEROCHROMATIN ; PROTEINS ; GENES ; ACTIVATION
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/104497
专题	生命科学与技术学院_特聘教授组_杨力组生命科学与技术学院_特聘教授组_陈玲玲组
通讯作者	Chen, Ling-Ling
作者单位	1.Chinese Acad Sci, Univ Chinese Acad Sci, State Key Lab Mol Biol,Shanghai Key Lab Mol Andro, CAS Ctr Excellence Mol Cell Sci,Shanghai Inst Bio, 320 Yueyang Rd, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai Inst Nutr & Hlth,Shanghai Inst Biol Sci, CAS Key Lab Computat Biol,CAS MPG Partner Inst Co, 320 Yueyang Rd, Shanghai 200031, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, 100 Haike Rd, Shanghai 201210, Peoples R China 4.Massachusetts Gen Hosp, Dept Pathol, Boston, MA 02114 USA 5.Harvard Med Sch, Boston, MA 02114 USA
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Yao, Run-Wen,Xu, Guang,Wang, Ying,et al. Nascent Pre-rRNA Sorting via Phase Separation Drives the Assembly of Dense Fibrillar Components in the Human Nucleolus[J]. MOLECULAR CELL,2019,76(5):767-+.
APA	Yao, Run-Wen.,Xu, Guang.,Wang, Ying.,Shan, Lin.,Luan, Peng-Fei.,...&Chen, Ling-Ling.(2019).Nascent Pre-rRNA Sorting via Phase Separation Drives the Assembly of Dense Fibrillar Components in the Human Nucleolus.MOLECULAR CELL,76(5),767-+.
MLA	Yao, Run-Wen,et al."Nascent Pre-rRNA Sorting via Phase Separation Drives the Assembly of Dense Fibrillar Components in the Human Nucleolus".MOLECULAR CELL 76.5(2019):767-+.