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| Nascent Pre-rRNA Sorting via Phase Separation Drives the Assembly of Dense Fibrillar Components in the Human Nucleolus | |
| 2019-12-05 | |
| Source Publication | MOLECULAR CELL
 |
| ISSN | 1097-2765 |
| EISSN | 1097-4164 |
| Volume | 76Issue:5Pages:767-+ |
| Status | 已发表 |
| DOI | 10.1016/j.molcel.2019.08.014 |
| Abstract | Fibrillar centers (FCs) and dense fibrillar components (DFCs) are essential morphologically distinct sub-regions of mammalian cell nucleoli for rDNA transcription and pre-rRNA processing. Here, we report that a human nucleolus consists of several dozen FC/DFC units, each containing 2-3 transcriptionally active rDNAs at the FC/DFC border. Pre-rRNA processing factors, such as fibrillarin (FBL), form 18-24 clusters that further assemble into the DFC surrounding the FC. Mechanistically, the 5' end of nascent 47S pre-rRNA binds co-transcriptionally to the RNA-binding domain of FBL. FBL diffuses to the DFC, where local self-association via its glycine- and arginine-rich (GAR) domain forms phase-separated clusters to immobilize FBL-interacting pre-rRNA, thus promoting directional traffic of nascent pre-rRNA while facilitating pre-rRNA processing and DFC formation. These results unveil FC/DFC ultrastructures in nucleoli and suggest a conceptual framework for considering nascent RNA sorting using multivalent interactions of their binding proteins. |
| URL | 查看原文 |
| Indexed By | SCI ; SCIE |
| Language | 英语 |
| Funding Project | HHMI[55008728] |
| WOS Research Area | Biochemistry & Molecular Biology ; Cell Biology |
| WOS Subject | Biochemistry & Molecular Biology ; Cell Biology |
| WOS ID | WOS:000500937100009 |
| Publisher | CELL PRESS |
| WOS Keyword | POLYMERASE-I TRANSCRIPTION ; QUANTITATIVE-ANALYSIS ; ORGANIZER REGIONS ; SSU PROCESSOME ; BOX C/D ; BINDING ; HETEROCHROMATIN ; PROTEINS ; GENES ; ACTIVATION |
| Original Document Type | Article |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/104497 |
| Collection | 生命科学与技术学院_特聘教授组_杨力组 生命科学与技术学院_特聘教授组_陈玲玲组 |
| Corresponding Author | Chen, Ling-Ling |
| Affiliation | 1.Chinese Acad Sci, Univ Chinese Acad Sci, State Key Lab Mol Biol,Shanghai Key Lab Mol Andro, CAS Ctr Excellence Mol Cell Sci,Shanghai Inst Bio, 320 Yueyang Rd, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai Inst Nutr & Hlth,Shanghai Inst Biol Sci, CAS Key Lab Computat Biol,CAS MPG Partner Inst Co, 320 Yueyang Rd, Shanghai 200031, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, 100 Haike Rd, Shanghai 201210, Peoples R China 4.Massachusetts Gen Hosp, Dept Pathol, Boston, MA 02114 USA 5.Harvard Med Sch, Boston, MA 02114 USA |
| Corresponding Author Affilication | School of Life Science and Technology |
| Recommended Citation GB/T 7714 | Yao, Run-Wen,Xu, Guang,Wang, Ying,et al. Nascent Pre-rRNA Sorting via Phase Separation Drives the Assembly of Dense Fibrillar Components in the Human Nucleolus[J]. MOLECULAR CELL,2019,76(5):767-+. |
| APA | Yao, Run-Wen.,Xu, Guang.,Wang, Ying.,Shan, Lin.,Luan, Peng-Fei.,...&Chen, Ling-Ling.(2019).Nascent Pre-rRNA Sorting via Phase Separation Drives the Assembly of Dense Fibrillar Components in the Human Nucleolus.MOLECULAR CELL,76(5),767-+. |
| MLA | Yao, Run-Wen,et al."Nascent Pre-rRNA Sorting via Phase Separation Drives the Assembly of Dense Fibrillar Components in the Human Nucleolus".MOLECULAR CELL 76.5(2019):767-+. |
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