Phosphorylation of Threonine(343) Is Crucial for OCT4 Interaction with SOX2 in the Maintenance of Mouse Embryonic Stem Cell Pluripotency

doi:10.1016/j.stemcr.2017.09.001

	Phosphorylation of Threonine(343) Is Crucial for OCT4 Interaction with SOX2 in the Maintenance of Mouse Embryonic Stem Cell Pluripotency
	Abulaiti, Xianmixinuer 1; Zhang, Han2,3,4,5 ; Wang, Aifang 2,4; Li, Na2,3,4 ; Li, Yang 1; Wang, Chenchen 1,2,4; Du, Xiaojuan 1; Li, Lingsong1,2,3,4,5
	2017-11-14
发表期刊	STEM CELL REPORTS (IF:5.9[JCR-2023],5.9[5-Year])
ISSN	2213-6711
卷号	9 期号:5 页码:1630-1641
发表状态	已发表
DOI	10.1016/j.stemcr.2017.09.001
摘要	OCT4 is required to maintain the pluripotency of embryonic stem cells (ESCs); yet, overdose-expression of OCT4 induces ESC differentiation toward primitive endoderm. The molecular mechanism underlying this differentiation switch is not fully understood. Here, we found that substitution of threonine(343) by alanine (T343A), but not aspartic acid (T343D), caused a significant loss of OCT4-phosphorylation signal in ESCs. Loss of such OCT4-phosphorylation compromises its interaction with SOX2 but promotes interaction with SOX17. We therefore propose that threonine(343)-based OCT4-phosphorylation is crucial for the maintenance of ESC pluripotency. This OCT4-phosphorylation-based mechanism may provide insight into the regulation of lineage specification during early embryonic development.
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31671217] ; National Natural Science Foundation of China[31000653] ; National Natural Science Foundation of China[31571517]
WOS研究方向	Cell Biology
WOS类目	Cell & Tissue Engineering ; Cell Biology
WOS记录号	WOS:000415139400025
出版者	CELL PRESS
WOS关键词	POU DOMAIN ; SELF-RENEWAL ; PREIMPLANTATION EMBRYO ; LINEAGE SPECIFIERS ; ES CELLS ; EXPRESSION ; NANOG ; PHOSPHOPROTEOME ; DIFFERENTIATION ; CIRCUITRY
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/10029
专题	生命科学与技术学院生命科学与技术学院_特聘教授组_李凌松组生命科学与技术学院_硕士生生命科学与技术学院_博士生
通讯作者	Wang, Chenchen; Li, Lingsong
作者单位	1.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Cell Biol, Beijing 100191, Peoples R China 2.Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai 201210, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Abulaiti, Xianmixinuer,Zhang, Han,Wang, Aifang,et al. Phosphorylation of Threonine(343) Is Crucial for OCT4 Interaction with SOX2 in the Maintenance of Mouse Embryonic Stem Cell Pluripotency[J]. STEM CELL REPORTS,2017,9(5):1630-1641.
APA	Abulaiti, Xianmixinuer.,Zhang, Han.,Wang, Aifang.,Li, Na.,Li, Yang.,...&Li, Lingsong.(2017).Phosphorylation of Threonine(343) Is Crucial for OCT4 Interaction with SOX2 in the Maintenance of Mouse Embryonic Stem Cell Pluripotency.STEM CELL REPORTS,9(5),1630-1641.
MLA	Abulaiti, Xianmixinuer,et al."Phosphorylation of Threonine(343) Is Crucial for OCT4 Interaction with SOX2 in the Maintenance of Mouse Embryonic Stem Cell Pluripotency".STEM CELL REPORTS 9.5(2017):1630-1641.