Detection of 2,2 '-Azobis(2-amidinopropane) Dihydrochloride in Polyvinylpyrrolidone by Capillary Electrophoresis with Field-Amplified Sample Injection

doi:10.1007/s10337-019-03765-2

	Detection of 2,2 '-Azobis(2-amidinopropane) Dihydrochloride in Polyvinylpyrrolidone by Capillary Electrophoresis with Field-Amplified Sample Injection
	Lou, Chunli1,2,3 ; Zheng, Mengmeng 2,3; Xu, Yao 2,3; Shen, Yuting 2,3; Kang, Jingwu1,2,3
	2019-10
发表期刊	CHROMATOGRAPHIA (IF:1.2[JCR-2023],1.7[5-Year])
ISSN	0009-5893
卷号	82 期号:10 页码:1579-1583
发表状态	已发表
DOI	10.1007/s10337-019-03765-2
摘要	A sensitive method was developed for detecting 2,2 '-azobis(2-amidinopropane) dihydrochloride in polyvinylpyrrolidone by capillary electrophoresis with indirect UV detection. The detection sensitivity was improved significantly using the field-amplified sample injection technique. The background electrolyte was composed of 25 mM imidazole-HCl solution (pH 4.0), in which imidazole acted as the cation probe for the indirect detection at UV 214 nm. Effects of the experimental parameters including pH, the concentration of imidazole as well as the sample injection time on sample preconcentration were investigated and optimized. Under the optimized conditions, baseline separation of 2,2 '-azobis(2-amidinopropane) ion with other ions in the polyvinylpyrrolidone samples was achieved within 8 min. The RSD% for the repeatability of the migration time and the peak area were determined as 0.95% and 3.47%, respectively. The limit of detection (LOD, S/N = 3) and the limit of quantitation (LOQ, S/N = 10) were determined as 1.11 x 10(-5) M and 2.77 x 10(-5) M, respectively. The measured recovery at three concentration levels was in the range of 101-104%. This method was successfully applied to detect 2,2 '-azobis(2-amidinopropane) in the commercial polyvinylpyrrolidone samples.
关键词	Capillary electrophoresis Field-amplified sample injection Polyvinylpyrrolidone 2 2 '-Azobis(2-amidinopropane) dihydrochloride
收录类别	SCI ; SCIE
语种	英语
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDB20020200]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000485313700014
出版者	SPRINGER HEIDELBERG
WOS关键词	SENSITIVITY ENHANCEMENT ; STACKING ; PRECONCENTRATION ; ANIONS
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/80227
专题	物质科学与技术学院_硕士生物质科学与技术学院_特聘教授组_康经武组
通讯作者	Kang, Jingwu
作者单位	1.ShanghaiTech Univ, Sch Phys Sci & Technol, Haike Rd 100, Shanghai 200120, Peoples R China 2.Chinese Acad Sci, State Key Lab Bioorgan & Nat Prod Chem, Ctr Excellence Mol Synth, Shanghai Inst Organ Chem, 345 Lingling Lu, Shanghai 200032, Peoples R China 3.Univ Chinese Acad Sci, Yuquan Rd 19, Beijing 100049, Peoples R China
第一作者单位	物质科学与技术学院
通讯作者单位	物质科学与技术学院
第一作者的第一单位	物质科学与技术学院
推荐引用方式 GB/T 7714	Lou, Chunli,Zheng, Mengmeng,Xu, Yao,et al. Detection of 2,2 '-Azobis(2-amidinopropane) Dihydrochloride in Polyvinylpyrrolidone by Capillary Electrophoresis with Field-Amplified Sample Injection[J]. CHROMATOGRAPHIA,2019,82(10):1579-1583.
APA	Lou, Chunli,Zheng, Mengmeng,Xu, Yao,Shen, Yuting,&Kang, Jingwu.(2019).Detection of 2,2 '-Azobis(2-amidinopropane) Dihydrochloride in Polyvinylpyrrolidone by Capillary Electrophoresis with Field-Amplified Sample Injection.CHROMATOGRAPHIA,82(10),1579-1583.
MLA	Lou, Chunli,et al."Detection of 2,2 '-Azobis(2-amidinopropane) Dihydrochloride in Polyvinylpyrrolidone by Capillary Electrophoresis with Field-Amplified Sample Injection".CHROMATOGRAPHIA 82.10(2019):1579-1583.