Plk4 Regulates Centriole Asymmetry and Spindle Orientation in Neural Stem Cells

doi:10.1016/j.devcel.2019.04.036

	Plk4 Regulates Centriole Asymmetry and Spindle Orientation in Neural Stem Cells
	Gambarotto, Davide 1,5; Pennetier, Carole 1; Ryniawec, John M.2; Buster, Daniel W.2; Gogendeau, Delphine 1; Goupil, Alix 1; Nano, Maddalena 1; Simon, Anthony 1; Blanc, Damien 3; Racine, Victor 3; Kimata, Yuu4 ; Rogers, Gregory C.2; Basto, Renata 1
	2019-07
发表期刊	DEVELOPMENTAL CELL (IF:10.7[JCR-2023],11.5[5-Year])
ISSN	1534-5807
卷号	50 期号:1 页码:11-+
发表状态	已发表
DOI	10.1016/j.devcel.2019.04.036
摘要	Defects in mitotic spindle orientation (MSO) disrupt the organization of stem cell niches impacting tissue morphogenesis and homeostasis. Mutations in centrosome genes reduce MSO fidelity, leading to tissue dysplasia and causing several diseases such as microcephaly, dwarfism, and cancer. Whether these mutations perturb spindle orientation solely by affecting astral microtubule nucleation or whether centrosome proteins have more direct functions in regulatingMSO is unknown. To investigate this question, we analyzed the consequences of deregulating Plk4 (the master centriole duplication kinase) activity in Drosophila asymmetrically dividing neural stem cells. We found that Plk4 functions upstream of MSO control, orchestrating centriole symmetry breaking and consequently centrosome positioning. Mechanistically, we show that Plk4 acts through Spd2 phosphorylation, which induces centriole release from the apical cortex. Overall, this work not only reveals a role for Plk4 in regulating centrosome function but also links the centrosome biogenesis machinery with the MSO apparatus.
收录类别	SCI ; SCIE
语种	英语
资助项目	NIGMS[R01 GM110166] ; NIGMS[GM126035]
WOS研究方向	Cell Biology ; Developmental Biology
WOS类目	Cell Biology ; Developmental Biology
WOS记录号	WOS:000473266600006
出版者	CELL PRESS
WOS关键词	CENTROSOME AMPLIFICATION ; FLUORESCENCE MICROSCOPY ; MASTER REGULATOR ; DROSOPHILA ; PROTEIN ; LOCALIZATION ; DUPLICATION ; EXPRESSION ; MECHANISM ; DIVISION
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/61128
专题	生命科学与技术学院_PI研究组_Yuu Kimita组
通讯作者	Rogers, Gregory C.; Basto, Renata
作者单位	1.PSL Res Univ, Inst Curie, CNRS, UMR144,Biol Centrosomes & Genet Instabil Lab, F-75005 Paris, France 2.Univ Arizona, Canc Ctr, Dept Cellular & Mol Med, Tucson, AZ 85724 USA 3.QuantaCell, 2 Allee Doyen Georges Brus, F-33600 Pessac, France 4.ShanghaiTech Univ, Sch Life Sci & Technol, 393 Middle Huaxia Rd, Shanghai 201210, Peoples R China 5.Univ Geneva, Dept Cell Biol, Sci 3, Geneva, Switzerland
推荐引用方式 GB/T 7714	Gambarotto, Davide,Pennetier, Carole,Ryniawec, John M.,et al. Plk4 Regulates Centriole Asymmetry and Spindle Orientation in Neural Stem Cells[J]. DEVELOPMENTAL CELL,2019,50(1):11-+.
APA	Gambarotto, Davide.,Pennetier, Carole.,Ryniawec, John M..,Buster, Daniel W..,Gogendeau, Delphine.,...&Basto, Renata.(2019).Plk4 Regulates Centriole Asymmetry and Spindle Orientation in Neural Stem Cells.DEVELOPMENTAL CELL,50(1),11-+.
MLA	Gambarotto, Davide,et al."Plk4 Regulates Centriole Asymmetry and Spindle Orientation in Neural Stem Cells".DEVELOPMENTAL CELL 50.1(2019):11-+.