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ShanghaiTech University Knowledge Management System
The structural and functional analysis of mycobacteria cysteine desulfurase-loaded encapsulin | |
2024-12-19 | |
发表期刊 | COMMUNICATIONS BIOLOGY (IF:5.2[JCR-2023],5.6[5-Year]) |
ISSN | 2399-3642 |
EISSN | 2399-3642 |
卷号 | 7期号:1 |
发表状态 | 已发表 |
DOI | 10.1038/s42003-024-07299-8 |
摘要 | Encapsulin nanocompartments loaded with dedicated cargo proteins via unique targeting peptides, play a key role in stress resistance, iron storage and natural product biosynthesis. Mmp1 and cysteine desulfurase (Enc-CD) have been identified as the most abundant representatives of family 2 encapsulin systems. However, the molecular assembly, catalytic mechanism, and physiological functions of the Mmp1 encapsulin system have not been studied in detail. Here we isolate and characterize an Enc-CD-loaded Mmp1 encapsulin system from Mycobacterium smegmatis mc2155. The cryo-EM structure of the Mmp1 encapsulin and the crystal structure of the naked cargo Enc-CD have been determined. The structure shows that the Mmp1 protomer assembles two conformation models, the icosahedron (T = 1) and homodecamer, with the resolution of 2.60 & Aring; and 2.69 & Aring;. The Enc-CD at 2.10 & Aring; resolution is dimeric and loaded into the Mmp1 (T = 1) encapsulin through the N-terminal long disordered region. Mmp1 encapsulin protects Enc-CD against oxidation as well as to maintain structural stability. These studies provide new insights into the mechanism by which Enc-CD-loaded encapsulin stores sulfur and provides a framework for discovery of new anti-mycobacterial therapeutics. |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | National Natural Science Foundation of China (National Science Foundation of China)[ |
WOS研究方向 | Life Sciences & Biomedicine - Other Topics ; Science & Technology - Other Topics |
WOS类目 | Biology ; Multidisciplinary Sciences |
WOS记录号 | WOS:001381238200007 |
出版者 | NATURE PORTFOLIO |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/471006 |
专题 | 免疫化学研究所 生命科学与技术学院 免疫化学研究所_特聘教授组_饶子和组 生命科学与技术学院_博士生 生命科学与技术学院_本科生 |
通讯作者 | Gao, Yan; Liu, Xiang; Rao, Zihe |
作者单位 | 1.Nankai Univ, Coll Life Sci, Frontiers Sci Ctr Cell Responses, State Key Lab Med Chem Biol, Tianjin, Peoples R China 2.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Sch Life Sci & Technol, Shanghai, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 4.Tianjin Univ, Sch Life Sci, Tianjin, Peoples R China 5.Univ Queensland, Sch Chem & Mol Biosci, Brisbane, Australia 6.Tsinghua Univ, Lab Struct Biol, Beijing, Peoples R China |
通讯作者单位 | 免疫化学研究所; 生命科学与技术学院 |
推荐引用方式 GB/T 7714 | Tang, Yanting,Liu, Yanyan,Zhang, Mingjing,et al. The structural and functional analysis of mycobacteria cysteine desulfurase-loaded encapsulin[J]. COMMUNICATIONS BIOLOGY,2024,7(1). |
APA | Tang, Yanting.,Liu, Yanyan.,Zhang, Mingjing.,Lan, Weiqi.,Ma, Mengyuan.,...&Rao, Zihe.(2024).The structural and functional analysis of mycobacteria cysteine desulfurase-loaded encapsulin.COMMUNICATIONS BIOLOGY,7(1). |
MLA | Tang, Yanting,et al."The structural and functional analysis of mycobacteria cysteine desulfurase-loaded encapsulin".COMMUNICATIONS BIOLOGY 7.1(2024). |
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