消息
×
loading..
The structural and functional analysis of mycobacteria cysteine desulfurase-loaded encapsulin
2024-12-19
发表期刊COMMUNICATIONS BIOLOGY (IF:5.2[JCR-2023],5.6[5-Year])
ISSN2399-3642
EISSN2399-3642
卷号7期号:1
发表状态已发表
DOI10.1038/s42003-024-07299-8
摘要

Encapsulin nanocompartments loaded with dedicated cargo proteins via unique targeting peptides, play a key role in stress resistance, iron storage and natural product biosynthesis. Mmp1 and cysteine desulfurase (Enc-CD) have been identified as the most abundant representatives of family 2 encapsulin systems. However, the molecular assembly, catalytic mechanism, and physiological functions of the Mmp1 encapsulin system have not been studied in detail. Here we isolate and characterize an Enc-CD-loaded Mmp1 encapsulin system from Mycobacterium smegmatis mc2155. The cryo-EM structure of the Mmp1 encapsulin and the crystal structure of the naked cargo Enc-CD have been determined. The structure shows that the Mmp1 protomer assembles two conformation models, the icosahedron (T = 1) and homodecamer, with the resolution of 2.60 & Aring; and 2.69 & Aring;. The Enc-CD at 2.10 & Aring; resolution is dimeric and loaded into the Mmp1 (T = 1) encapsulin through the N-terminal long disordered region. Mmp1 encapsulin protects Enc-CD against oxidation as well as to maintain structural stability. These studies provide new insights into the mechanism by which Enc-CD-loaded encapsulin stores sulfur and provides a framework for discovery of new anti-mycobacterial therapeutics.

URL查看原文
收录类别SCI
语种英语
资助项目National Natural Science Foundation of China (National Science Foundation of China)[
WOS研究方向Life Sciences & Biomedicine - Other Topics ; Science & Technology - Other Topics
WOS类目Biology ; Multidisciplinary Sciences
WOS记录号WOS:001381238200007
出版者NATURE PORTFOLIO
文献类型期刊论文
条目标识符https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/471006
专题免疫化学研究所
生命科学与技术学院
免疫化学研究所_特聘教授组_饶子和组
生命科学与技术学院_博士生
生命科学与技术学院_本科生
通讯作者Gao, Yan; Liu, Xiang; Rao, Zihe
作者单位
1.Nankai Univ, Coll Life Sci, Frontiers Sci Ctr Cell Responses, State Key Lab Med Chem Biol, Tianjin, Peoples R China
2.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Sch Life Sci & Technol, Shanghai, Peoples R China
3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
4.Tianjin Univ, Sch Life Sci, Tianjin, Peoples R China
5.Univ Queensland, Sch Chem & Mol Biosci, Brisbane, Australia
6.Tsinghua Univ, Lab Struct Biol, Beijing, Peoples R China
通讯作者单位免疫化学研究所;  生命科学与技术学院
推荐引用方式
GB/T 7714
Tang, Yanting,Liu, Yanyan,Zhang, Mingjing,et al. The structural and functional analysis of mycobacteria cysteine desulfurase-loaded encapsulin[J]. COMMUNICATIONS BIOLOGY,2024,7(1).
APA Tang, Yanting.,Liu, Yanyan.,Zhang, Mingjing.,Lan, Weiqi.,Ma, Mengyuan.,...&Rao, Zihe.(2024).The structural and functional analysis of mycobacteria cysteine desulfurase-loaded encapsulin.COMMUNICATIONS BIOLOGY,7(1).
MLA Tang, Yanting,et al."The structural and functional analysis of mycobacteria cysteine desulfurase-loaded encapsulin".COMMUNICATIONS BIOLOGY 7.1(2024).
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
个性服务
查看访问统计
谷歌学术
谷歌学术中相似的文章
[Tang, Yanting]的文章
[Liu, Yanyan]的文章
[Zhang, Mingjing]的文章
百度学术
百度学术中相似的文章
[Tang, Yanting]的文章
[Liu, Yanyan]的文章
[Zhang, Mingjing]的文章
必应学术
必应学术中相似的文章
[Tang, Yanting]的文章
[Liu, Yanyan]的文章
[Zhang, Mingjing]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。