Isobutanol and 2-ketoisovalerate production by Klebsiella pneumoniae via a native pathway
2017-09
发表期刊METABOLIC ENGINEERING (IF:6.8[JCR-2023],7.6[5-Year])
ISSN1096-7176
卷号43页码:71-84
发表状态已发表
DOI10.1016/j.ymben.2017.07.003
摘要Isobutanol is a valuable chemical and is considered a new generation biofuel. Construction of isobutanol synthesis pathways in bacteria is a hot topic in isobutanol production. Here, we show that an isobutanol synthesis pathway exists naturally in Klebsiella pneumoniae; however, this pathway is dormant in the wild-type bacterium. K. pneumoniae is a 2,3-butanediol producer, and the synthesis pathways of 2,3-butanediol, valine and isobutanol all start from condensation of two pyruvate molecules to yield alpha-acetolactate. Inactivation of alpha-acetolactate decarboxylase (encoded by budA) resulted in alpha-acetolactate flowing into the valine pathway, which led to synthesis of isobutanol and 2-ketoisovalerate (a precursor of isobutanol). ldhA (lactate dehydrogenase) deletion further increased the isobutanol and 2-ketoisovalerate production. In the first step of this pathway, BudB (alpha-acetolactate synthase) was identified as responsible for most of the alpha-acetolactate synthesis. Complementation of ilvBN or ilvIH (isoenzymes of budB) both resulted in a remarkable increase in 2-ketoisovalerate production. Thus, alpha-acetolactate formation is the rate-limiting step of 2-ketoisovalerate production. ilvC (acetohydroxy acid isomeroreductase) and ilvD (dihydroxy acid dehydratase) were identified responsible for 2-ketoisovalerate synthesis from alpha-acetolactate. ipdC, which encodes an indole-3-pyruvate decarboxylase, was identified responsible for most of the isobutyraldehyde formation from 2-ketoisovalerate, and isobutanol production was increased 15.7 fold in the ipdC complementation strain, with a final titer of 2.45 g/L. Isobutanol dehydrogenase activity is distributed across multiple alcohol dehydrogenase enzymes expressed by K. pneumoniae. BudC, DhaT, DhaD and YqhD all had isobutanol dehydrogenase activity in vitro. YqhD uses NADPH as the coenzyme, while the other dehydrogenases use NADH. However, inactivating one or two of these dehydrogenases had no effect on isobutanol production in vivo with isobutyraldehyde as the substrate. These results reveal a novel method for biological production of isobutanol and 2-ketoisovalerate.
关键词2,3-butanediol Isobutanol 2-ketoisovalerate Klebsiella pneumoniae
收录类别SCI ; EI
语种英语
资助项目KRIBB Research Initiative Program[KGM2211531]
WOS研究方向Biotechnology & Applied Microbiology
WOS类目Biotechnology & Applied Microbiology
WOS记录号WOS:000410479600009
出版者ACADEMIC PRESS INC ELSEVIER SCIENCE
EI入藏号20173504088347
EI主题词Amino acids ; Bacteria ; Enzymes ; Synthesis (chemical)
EI分类号Chemical Reactions:802.2 ; Organic Compounds:804.1
WOS关键词ESCHERICHIA-COLI K-12 ; 2-KETOGLUCONIC ACID PRODUCTION ; GENE REPLACEMENT ; CORYNEBACTERIUM-GLUTAMICUM ; NUCLEOTIDE-SEQUENCE ; 1,3-PROPANEDIOL ; BIOSYNTHESIS ; OPERON ; SYNTHASE ; IDENTIFICATION
原始文献类型Article
引用统计
正在获取...
文献类型期刊论文
条目标识符https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/4643
专题生命科学与技术学院
生命科学与技术学院_特聘教授组_史吉平组
生命科学与技术学院_博士生
通讯作者Shi, Jiping; Hao, Jian
作者单位
1.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
2.Chinese Acad Sci, Shanghai Adv Res Inst, Lab Biorefinery, 99 Haike Rd, Shanghai 201210, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.KRIBB, Jeonbuk Branch Inst, Biorefinery Res Ctr, Jeongeup 580185, Jeonbuk, South Korea
第一作者单位生命科学与技术学院
第一作者的第一单位生命科学与技术学院
推荐引用方式
GB/T 7714
Gu, Jinjie,Zhou, Jidong,Zhang, Zhongxi,et al. Isobutanol and 2-ketoisovalerate production by Klebsiella pneumoniae via a native pathway[J]. METABOLIC ENGINEERING,2017,43:71-84.
APA Gu, Jinjie.,Zhou, Jidong.,Zhang, Zhongxi.,Kim, Chul Ho.,Jiang, Biao.,...&Hao, Jian.(2017).Isobutanol and 2-ketoisovalerate production by Klebsiella pneumoniae via a native pathway.METABOLIC ENGINEERING,43,71-84.
MLA Gu, Jinjie,et al."Isobutanol and 2-ketoisovalerate production by Klebsiella pneumoniae via a native pathway".METABOLIC ENGINEERING 43(2017):71-84.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
个性服务
查看访问统计
谷歌学术
谷歌学术中相似的文章
[Gu, Jinjie]的文章
[Zhou, Jidong]的文章
[Zhang, Zhongxi]的文章
百度学术
百度学术中相似的文章
[Gu, Jinjie]的文章
[Zhou, Jidong]的文章
[Zhang, Zhongxi]的文章
必应学术
必应学术中相似的文章
[Gu, Jinjie]的文章
[Zhou, Jidong]的文章
[Zhang, Zhongxi]的文章
相关权益政策
暂无数据
收藏/分享
文件名: 顾金杰 Isobutanol and 2-ketoisovalerate production by Klebsiella pneumoniae via a native pathway.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。