Precise Protein Photolithography (P-3): High Performance Biopatterning Using Silk Fibroin Light Chain as the Resist

doi:10.1002/advs.201700191

	Precise Protein Photolithography (P-3): High Performance Biopatterning Using Silk Fibroin Light Chain as the Resist
	Liu, Wanpeng 1; Zhou, Zhitao 2,3; Zhang, Shaoqing 1; Shi, Zhifeng 4; Tabarini, Justin 1; Lee, Woonsoo 1; Zhang, Yeshun 5; Corder, S. N. Gilbert 6; Li, Xinxin 2,3,7; Dong, Fei 4; Cheng, Liang 4; Liu, Mengkun 6; Kaplan, David L.8; Omenetto, Fiorenzo G.8; Zhang, Guozheng 5; Mao, Ying 4; Tao, Tiger H.1,2,3,7
	2017-09
发表期刊	ADVANCED SCIENCE
ISSN	2198-3844
卷号	4 期号:9
发表状态	已发表
DOI	10.1002/advs.201700191
摘要	Precise patterning of biomaterials has widespread applications, including drug release, degradable implants, tissue engineering, and regenerative medicine. Patterning of protein-based microstructures using UV-photolithography has been demonstrated using protein as the resist material. The Achilles heel of existing protein-based biophotoresists is the inevitable wide molecular weight distribution during the protein extraction/regeneration process, hindering their practical uses in the semiconductor industry where reliability and repeatability are paramount. A wafer-scale high resolution patterning of bio-microstructures using well-defined silk fibroin light chain as the resist material is presented showing unprecedent performances. The lithographic and etching performance of silk fibroin light chain resists are evaluated systematically and the underlying mechanisms are thoroughly discussed. The micropatterned silk structures are tested as cellular substrates for the successful spatial guidance of fetal neural stems cells seeded on the patterned substrates. The enhanced patterning resolution, the improved etch resistance, and the inherent biocompatibility of such protein-based photoresist provide new opportunities in fabricating large scale biocompatible functional microstructures.
关键词	biopatterning protein photolithography silk fibroin light chain
收录类别	SCI
语种	英语
资助项目	NSF under CMMI Division[1563422] ; NSF under CMMI Division[1562915]
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science
WOS类目	Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS记录号	WOS:000411148100025
出版者	WILEY
WOS关键词	RELEASE DRUG-DELIVERY ; BOMBYX-MORI ; FILMS ; FABRICATION ; NANOSCALE ; HEAVY ; MICROSTRUCTURES ; BIOMATERIALS ; AGGREGATION ; LITHOGRAPHY
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/4639
专题	物质科学与技术学院物质科学与技术学院_特聘教授组_陶虎组
通讯作者	Zhang, Guozheng; Mao, Ying; Tao, Tiger H.
作者单位	1.Univ Texas Austin, Dept Mech Engn, Austin, TX 78712 USA 2.Chinese Acad Sci, Shanghai Inst Microsyst & Informat Technol, State Key Lab Transducer Technol, Shanghai 200050, Peoples R China 3.Univ Chinese Sci Acad, Sch Grad Study, Beijing 100049, Peoples R China 4.Fudan Univ, Huashan Hosp, Dept Neurosurg, Wulumuqi Zhong Rd 12, Shanghai 200040, Peoples R China 5.Jiangsu Univ Sci & Technol, 2 Mengxi Rd, Zhenjiang 212003, Jiangsu, Peoples R China 6.SUNY Stony Brook, Dept Phys & Astron, Stony Brook, NY 11794 USA 7.ShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai 200031, Peoples R China 8.Tufts Univ, Dept Biomed Engn, Medford, MA 02155 USA
通讯作者单位	物质科学与技术学院
推荐引用方式 GB/T 7714	Liu, Wanpeng,Zhou, Zhitao,Zhang, Shaoqing,et al. Precise Protein Photolithography (P-3): High Performance Biopatterning Using Silk Fibroin Light Chain as the Resist[J]. ADVANCED SCIENCE,2017,4(9).
APA	Liu, Wanpeng.,Zhou, Zhitao.,Zhang, Shaoqing.,Shi, Zhifeng.,Tabarini, Justin.,...&Tao, Tiger H..(2017).Precise Protein Photolithography (P-3): High Performance Biopatterning Using Silk Fibroin Light Chain as the Resist.ADVANCED SCIENCE,4(9).
MLA	Liu, Wanpeng,et al."Precise Protein Photolithography (P-3): High Performance Biopatterning Using Silk Fibroin Light Chain as the Resist".ADVANCED SCIENCE 4.9(2017).