Cell-free synthetic biology for in vitro biosynthesis of pharmaceutical natural products

doi:10.1016/j.synbio.2018.02.002

	Cell-free synthetic biology for in vitro biosynthesis of pharmaceutical natural products
	Li, Jian; Zhang, Lingkai; Liu, Wanqiu
	2018-06
发表期刊	ARCHIVAL SCIENCE (IF:1.4[JCR-2023],1.8[5-Year])
ISSN	1389-0166
卷号	3 期号:2 页码:83-89
发表状态	已发表
DOI	10.1016/j.synbio.2018.02.002
摘要	Natural products with significant biological activities continuously act as rich sources for drug discovery and development. To harness the potential of these valuable compounds, robust methods need to be developed for their rapid and sustainable production. Cell-free biosynthesis of pharmaceutical natural products by in vitro reconstruction of the entire biosynthetic pathways represents one such solution. In this review, we focus on in vitro biosynthesis of two important classes of natural products, polyketides (PKs) and nonribosomal peptides (NRPs). First, we summarize purified enzyme-based systems for the biosynthesis of PKs, NRPs, and PK/NRP hybrids. Then, we introduce the cell-free protein synthesis (CFPS)-based technology for natural product production. With that, we discuss challenges and opportunities of cell-free synthetic biology for in vitro biosynthesis of natural products. (c) 2018 The Authors. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co.
关键词	Cell-free synthetic biology Purified enzymes Cell-free protein synthesis Polyketides Nonribosomal peptides In vitro biosynthesis Natural products
收录类别	ESCI ; SCIE
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000441188400002
出版者	KEAI PUBLISHING LTD
WOS关键词	FREE PROTEIN-SYNTHESIS ; PEPTIDYL NUCLEOSIDE ANTIBIOTICS ; ESCHERICHIA-COLI ; ASSEMBLY-LINE ; HETEROLOGOUS EXPRESSION ; PACIDAMYCIN GROUP ; YERSINIA-PESTIS ; CHEMICAL LOGIC ; FREE PLATFORM ; POLYKETIDE
原始文献类型	Review
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/45764
专题	物质科学与技术学院_PI研究组_李健组物质科学与技术学院_硕士生
通讯作者	Li, Jian
作者单位	ShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai 201210, Peoples R China
第一作者单位	物质科学与技术学院
通讯作者单位	物质科学与技术学院
第一作者的第一单位	物质科学与技术学院
推荐引用方式 GB/T 7714	Li, Jian,Zhang, Lingkai,Liu, Wanqiu. Cell-free synthetic biology for in vitro biosynthesis of pharmaceutical natural products[J]. ARCHIVAL SCIENCE,2018,3(2):83-89.
APA	Li, Jian,Zhang, Lingkai,&Liu, Wanqiu.(2018).Cell-free synthetic biology for in vitro biosynthesis of pharmaceutical natural products.ARCHIVAL SCIENCE,3(2),83-89.
MLA	Li, Jian,et al."Cell-free synthetic biology for in vitro biosynthesis of pharmaceutical natural products".ARCHIVAL SCIENCE 3.2(2018):83-89.