Cell-free synthetic biology for in vitro biosynthesis of pharmaceutical natural products
Li, Jian; Zhang, Lingkai; Liu, Wanqiu
2018-06
Source PublicationARCHIVAL SCIENCE
ISSN1389-0166
Volume3Issue:2Pages:83-89
Status已发表
DOI10.1016/j.synbio.2018.02.002
AbstractNatural products with significant biological activities continuously act as rich sources for drug discovery and development. To harness the potential of these valuable compounds, robust methods need to be developed for their rapid and sustainable production. Cell-free biosynthesis of pharmaceutical natural products by in vitro reconstruction of the entire biosynthetic pathways represents one such solution. In this review, we focus on in vitro biosynthesis of two important classes of natural products, polyketides (PKs) and nonribosomal peptides (NRPs). First, we summarize purified enzyme-based systems for the biosynthesis of PKs, NRPs, and PK/NRP hybrids. Then, we introduce the cell-free protein synthesis (CFPS)-based technology for natural product production. With that, we discuss challenges and opportunities of cell-free synthetic biology for in vitro biosynthesis of natural products. (c) 2018 The Authors. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co.
KeywordCell-free synthetic biology Purified enzymes Cell-free protein synthesis Polyketides Nonribosomal peptides In vitro biosynthesis Natural products
Indexed ByESCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000441188400002
PublisherKEAI PUBLISHING LTD
WOS KeywordFREE PROTEIN-SYNTHESIS ; PEPTIDYL NUCLEOSIDE ANTIBIOTICS ; ESCHERICHIA-COLI ; ASSEMBLY-LINE ; HETEROLOGOUS EXPRESSION ; PACIDAMYCIN GROUP ; YERSINIA-PESTIS ; CHEMICAL LOGIC ; FREE PLATFORM ; POLYKETIDE
Original Document TypeReview
Citation statistics
Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttps://kms.shanghaitech.edu.cn/handle/2MSLDSTB/45764
Collection物质科学与技术学院_PI研究组_李健组
物质科学与技术学院_硕士生
Corresponding AuthorLi, Jian
AffiliationShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai 201210, Peoples R China
First Author AffilicationSchool of Physical Science and Technology
Corresponding Author AffilicationSchool of Physical Science and Technology
First Signature AffilicationSchool of Physical Science and Technology
Recommended Citation
GB/T 7714
Li, Jian,Zhang, Lingkai,Liu, Wanqiu. Cell-free synthetic biology for in vitro biosynthesis of pharmaceutical natural products[J]. ARCHIVAL SCIENCE,2018,3(2):83-89.
APA Li, Jian,Zhang, Lingkai,&Liu, Wanqiu.(2018).Cell-free synthetic biology for in vitro biosynthesis of pharmaceutical natural products.ARCHIVAL SCIENCE,3(2),83-89.
MLA Li, Jian,et al."Cell-free synthetic biology for in vitro biosynthesis of pharmaceutical natural products".ARCHIVAL SCIENCE 3.2(2018):83-89.
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