PPR polyadenylation factor defines mitochondrial mRNA identity and stability in trypanosomes

doi:10.15252/embj.201796808

	PPR polyadenylation factor defines mitochondrial mRNA identity and stability in trypanosomes
	Zhang, Liye1,2,5 ; Sement, Francois M.1; Suematsu, Takuma 1; Yu, Tian 1; Monti, Stefano 2; Huang, Lan 3; Aphasizhev, Ruslan 1,4; Aphasizheva, Inna 1
	2017-08-15
发表期刊	EMBO JOURNAL (IF:9.4[JCR-2023],11.0[5-Year])
ISSN	0261-4189
卷号	36 期号:16 页码:2435-2454
发表状态	已发表
DOI	10.15252/embj.201796808
摘要	In Trypanosoma brucei, most mitochondrial mRNAs undergo internal changes by RNA editing and 30 end modifications. The temporally separated and functionally distinct modifications are manifested by adenylation prior to editing, and by post-editing extension of a short A-tail into a long A/U-heteropolymer. The A-tail stabilizes partially and fully edited mRNAs, while the A/U-tail enables mRNA binding to the ribosome. Here, we identify an essential pentatricopeptide repeat-containing RNA binding protein, kinetoplast polyadenylation factor 3 (KPAF3), and demonstrate its role in protecting pre-mRNA against degradation by the processome. We show that KPAF3 recruits KPAP1 poly(A) polymerase to the 30 terminus, thus leading to pre-mRNA stabilization, or decay depending on the occurrence and extent of editing. In vitro, KPAF3 stimulates KPAP1 activity and inhibits mRNA uridylation by RET1 TUTase. Our findings indicate that KPAF3 selectively directs pre-mRNA toward adenylation rather than uridylation, which is a default post-trimming modification characteristic of ribosomal and guide RNAs. As a quality control mechanism, KPAF3 binding ensures that mRNAs entering the editing pathway are adenylated and, therefore, competent for post-editing A/U-tailing and translational activation.
关键词	pentatricopeptide repeat polyadenylation RNA editing RNA stability Trypanosoma
收录类别	SCI
语种	英语
资助项目	NIH[AI113157] ; NIH[AI091914] ; NIH[AI101057] ; NIH[GM074830] ; NIH[GM106003]
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Cell Biology
WOS记录号	WOS:000407786500011
出版者	WILEY
WOS关键词	PENTATRICOPEPTIDE REPEAT PROTEINS ; INDUCIBLE EXPRESSION SYSTEM ; BRUCEI MITOCHONDRIA ; GUIDE RNAS ; COMPLEX ; BINDING ; TURNOVER ; RECOGNITION ; TRANSLATION ; TRANSCRIPTS
原始文献类型	Article
通讯作者	Aphasizheva, Inna
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/4520
专题	生命科学与技术学院_PI研究组_张力烨组
通讯作者	Aphasizheva, Inna
作者单位	1.Boston Univ, Sch Dent Med, Dept Mol & Cell Biol, Boston, MA 02215 USA 2.Boston Univ, Sch Med, Sect Computat Biomed, Boston, MA 02118 USA 3.Univ Calif Irvine, Sch Med, Dept Physiol & Biophys, Irvine, CA 92717 USA 4.Boston Univ, Sch Med, Dept Biochem, Boston, MA 02118 USA 5.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
第一作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Zhang, Liye,Sement, Francois M.,Suematsu, Takuma,et al. PPR polyadenylation factor defines mitochondrial mRNA identity and stability in trypanosomes[J]. EMBO JOURNAL,2017,36(16):2435-2454.
APA	Zhang, Liye.,Sement, Francois M..,Suematsu, Takuma.,Yu, Tian.,Monti, Stefano.,...&Aphasizheva, Inna.(2017).PPR polyadenylation factor defines mitochondrial mRNA identity and stability in trypanosomes.EMBO JOURNAL,36(16),2435-2454.
MLA	Zhang, Liye,et al."PPR polyadenylation factor defines mitochondrial mRNA identity and stability in trypanosomes".EMBO JOURNAL 36.16(2017):2435-2454.