上海科技大学知识管理系统

Bioconjugation has been proved to be highly promising in biomedicine and biotechnology. However, controllable bioconjugation triggered by photo-crosslinking reaction in living organisms still suffers from low effectiveness and limited tissue penetration due to the loss of photon energy with short excitation wavelength. Herein, we proposed a generalized near-infrared (NIR) controlled strategy for spatiotemporal bioconjugation using upconversion nanocomposite functionalized with perfluorophenyl azide (PFPA). The ultraviolet emissions of upconversion nanocomposites excited by 808 nm light activate the PFPA mediated bioconjugation in biological system, which inherits the merits of high controllability and tissue penetration of NIR excitation and highly coupling efficiency of PFPA. For in vitro applications, the nanocomposite significantly enhances cell-biointerface conjugation efficiency and achieves intercellular bioconjugation for cell fusion. For in vivo bioconjugation, the nanocomposite also amplifies tumor targeted delivery with 10-fold increase in nanoparticle enrichment, and enables oriented immune activation in lymph node. The NIR-mediated bioconjugation method based on the developed nanocomposite provides a promising strategy with biomacromolecular universality, high selectivity and low invasiveness in living organisms that can serve as a new platform for disease treatment, tissue engineering, and other biomedical applications. © 2024 Elsevier B.V.