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Volumetric voltage imaging of neuronal populations in the mouse brain by confocal light-field microscopy
2024-10-01
发表期刊NATURE METHODS (IF:36.1[JCR-2023],45.6[5-Year])
ISSN1548-7091
EISSN1548-7105
发表状态已发表
DOI10.1038/s41592-024-02458-5
摘要

["Voltage imaging measures neuronal activity directly and holds promise for understanding information processing within individual neurons and across populations. However, imaging voltage over large neuronal populations has been challenging owing to the simultaneous requirements of high imaging speed and signal-to-noise ratio, large volume coverage and low photobleaching rate. Here, to overcome this challenge, we developed a confocal light-field microscope that surpassed the traditional limits in speed and noise performance by incorporating a speed-enhanced camera, a fast and robust scanning mechanism, laser-speckle-noise elimination and optimized light efficiency. With this method, we achieved simultaneous recording from more than 300 spiking neurons within an 800-mu m-diameter and 180-mu m-thick volume in the mouse cortex, for more than 20 min. By integrating the spatial and voltage activity profiles, we have mapped three-dimensional neural coordination patterns in awake mouse brains. Our method is robust for routine application in volumetric voltage imaging.","Confocal light-field microscopy allows volumetric voltage imaging in the mouse brain at speeds sufficiently high to extract the spiking activity of hundreds of neurons."]

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收录类别SCI
语种英语
资助项目STI2030-Major Projects (2021ZD0204503), National Natural Science Foundation of China (32125020 and 32321003), Shanghai Committee of Science and Technology, China (22JC1403101)[2021ZD0204503] ; National Science and Technology Innovation[
WOS研究方向Biochemistry & Molecular Biology
WOS类目Biochemical Research Methods
WOS记录号WOS:001328368000001
出版者NATURE PORTFOLIO
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文献类型期刊论文
条目标识符https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/436498
专题生命科学与技术学院
生命科学与技术学院_特聘教授组_熊志奇组
生命科学与技术学院_特聘教授组_王凯组
通讯作者Wang, Kai
作者单位
1.Chinese Acad Sci, Inst Neurosci, CAS Ctr Excellence Brain Sci & Intelligence Techno, Key Lab Brain Cognit & Brain Inspired Intelligence, Shanghai, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
4.Shanghai Ctr Brain Sci & Brain Inspired Intelligen, Shanghai, Peoples R China
通讯作者单位生命科学与技术学院
推荐引用方式
GB/T 7714
Bai, Lu,Cong, Lin,Shi, Ziqi,et al. Volumetric voltage imaging of neuronal populations in the mouse brain by confocal light-field microscopy[J]. NATURE METHODS,2024.
APA Bai, Lu.,Cong, Lin.,Shi, Ziqi.,Zhao, Yuchen.,Zhang, Yujie.,...&Wang, Kai.(2024).Volumetric voltage imaging of neuronal populations in the mouse brain by confocal light-field microscopy.NATURE METHODS.
MLA Bai, Lu,et al."Volumetric voltage imaging of neuronal populations in the mouse brain by confocal light-field microscopy".NATURE METHODS (2024).
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