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ShanghaiTech University Knowledge Management System
Volumetric voltage imaging of neuronal populations in the mouse brain by confocal light-field microscopy | |
2024-10-01 | |
发表期刊 | NATURE METHODS (IF:36.1[JCR-2023],45.6[5-Year]) |
ISSN | 1548-7091 |
EISSN | 1548-7105 |
发表状态 | 已发表 |
DOI | 10.1038/s41592-024-02458-5 |
摘要 | ["Voltage imaging measures neuronal activity directly and holds promise for understanding information processing within individual neurons and across populations. However, imaging voltage over large neuronal populations has been challenging owing to the simultaneous requirements of high imaging speed and signal-to-noise ratio, large volume coverage and low photobleaching rate. Here, to overcome this challenge, we developed a confocal light-field microscope that surpassed the traditional limits in speed and noise performance by incorporating a speed-enhanced camera, a fast and robust scanning mechanism, laser-speckle-noise elimination and optimized light efficiency. With this method, we achieved simultaneous recording from more than 300 spiking neurons within an 800-mu m-diameter and 180-mu m-thick volume in the mouse cortex, for more than 20 min. By integrating the spatial and voltage activity profiles, we have mapped three-dimensional neural coordination patterns in awake mouse brains. Our method is robust for routine application in volumetric voltage imaging.","Confocal light-field microscopy allows volumetric voltage imaging in the mouse brain at speeds sufficiently high to extract the spiking activity of hundreds of neurons."] |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | STI2030-Major Projects (2021ZD0204503), National Natural Science Foundation of China (32125020 and 32321003), Shanghai Committee of Science and Technology, China (22JC1403101)[2021ZD0204503] ; National Science and Technology Innovation[ |
WOS研究方向 | Biochemistry & Molecular Biology |
WOS类目 | Biochemical Research Methods |
WOS记录号 | WOS:001328368000001 |
出版者 | NATURE PORTFOLIO |
引用统计 | 正在获取...
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文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/436498 |
专题 | 生命科学与技术学院 生命科学与技术学院_特聘教授组_熊志奇组 生命科学与技术学院_特聘教授组_王凯组 |
通讯作者 | Wang, Kai |
作者单位 | 1.Chinese Acad Sci, Inst Neurosci, CAS Ctr Excellence Brain Sci & Intelligence Techno, Key Lab Brain Cognit & Brain Inspired Intelligence, Shanghai, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 4.Shanghai Ctr Brain Sci & Brain Inspired Intelligen, Shanghai, Peoples R China |
通讯作者单位 | 生命科学与技术学院 |
推荐引用方式 GB/T 7714 | Bai, Lu,Cong, Lin,Shi, Ziqi,et al. Volumetric voltage imaging of neuronal populations in the mouse brain by confocal light-field microscopy[J]. NATURE METHODS,2024. |
APA | Bai, Lu.,Cong, Lin.,Shi, Ziqi.,Zhao, Yuchen.,Zhang, Yujie.,...&Wang, Kai.(2024).Volumetric voltage imaging of neuronal populations in the mouse brain by confocal light-field microscopy.NATURE METHODS. |
MLA | Bai, Lu,et al."Volumetric voltage imaging of neuronal populations in the mouse brain by confocal light-field microscopy".NATURE METHODS (2024). |
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