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| Modular genetic design of multi-domain functional amyloids: insights into self-assembly and functional properties | |
| 2019-04-14 | |
| Source Publication | CHEMICAL SCIENCE
 |
| ISSN | 2041-6520 |
| Volume | 10Issue:14Pages:4004-4014 |
| Status | 已发表 |
| DOI | 10.1039/c9sc00208a |
| Abstract | Engineering functional amyloids through a modular genetic strategy represents new opportunities for creating multifunctional molecular materials with tailored structures and performance. Despite important advances, how fusion modules affect the self-assembly and functional properties of amyloids remains elusive. Here, using Escherichia coli curli as a model system, we systematically studied the effect of flanking domains on the structures, assembly kinetics and functions of amyloids. The designed amyloids were composed of E. coli biofilm protein CsgA (as amyloidogenic cores) and one or two flanking domains, consisting of chitin-binding domains (CBDs) from Bacillus circulans chitinase, and/or mussel foot proteins (Mfps). Incorporation of fusion domains did not disrupt the typical -sheet structures, but indeed affected assembly rate, morphology, and stiffness of resultant fibrils. Consequently, the CsgA-fusion fibrils, particularly those containing three domains, were much shorter than the CsgA-only fibrils. Furthermore, the stiffness of the resultant fibrils was heavily affected by the structural feature of fusion domains, with -sheet-containing domains tending to increase the Young's modulus while random coil domains decreasing the Young's modulus. In addition, fibrils containing CBD domains showed higher chitin-binding activity compared to their CBD-free counterparts. The CBD-CsgA-Mfp3 construct exhibited significantly lower binding activity than Mfp5-CsgA-CBD due to inappropriate folding of the CBD domain in the former construct, in agreement with results based upon molecular dynamics modeling. Our study provides new insights into the assembly and functional properties of designer amyloid proteins with increasing complex domain structures and lays the foundation for the future design of functional amyloid-based structures and molecular materials. |
| Indexed By | EI ; SCIE ; SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[U1532127] ; National Natural Science Foundation of China[31570972] |
| WOS Research Area | Chemistry |
| WOS Subject | Chemistry, Multidisciplinary |
| WOS ID | WOS:000467988600008 |
| Publisher | ROYAL SOC CHEMISTRY |
| EI Accession Number | 20191506765710 |
| EI Keywords | Bacteriology ; Chitin ; Elastic moduli ; Escherichia coli ; Glycoproteins ; Molecular dynamics ; Proteins ; Self assembly ; Stiffness |
| EI Classification Number | Structural Design, General:408.1 ; Biology:461.9 ; Physical Chemistry:801.4 ; Organic Compounds:804.1 ; Materials Science:951 |
| WOS Keyword | EXTRACELLULAR-MATRIX ; STRUCTURAL PREDICTIONS ; MECHANICAL-PROPERTIES ; FIBRILS ; A-BETA-42 ; DETERMINANTS ; BIOGENESIS ; ADHESIVES ; PROTEINS |
| Original Document Type | Article |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/40852 |
| Collection | 物质科学与技术学院_博士生 物质科学与技术学院_PI研究组_钟超组 物质科学与技术学院_硕士生 物质科学与技术学院_本科生 |
| Corresponding Author | Zhong, Chao |
| Affiliation | 1.ShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai 200120, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Organ Chem, Shanghai 200032, Peoples R China 4.MIT, Dept Biol Engn, 77 Massachusetts Ave, Cambridge, MA 02139 USA 5.Chinese Acad Sci, Shanghai Inst Organ Chem, Interdisciplinary Res Ctr Biol & Chem, Shanghai 200032, Peoples R China 6.MIT, Dept Elect Engn & Comp Sci, Dept Biol Engn, Cambridge, MA 02139 USA 7.Politecn Milan, Dipartimento Chim Mat & Ingn Chim G Natta, Piazza Leonardo Vinci 32, I-20133 Milan, Italy 8.Imperial Coll London, Dept Mat, London SW7 2AZ, England |
| First Author Affilication | School of Physical Science and Technology |
| Corresponding Author Affilication | School of Physical Science and Technology |
| First Signature Affilication | School of Physical Science and Technology |
| Recommended Citation GB/T 7714 | Cui, Mengkui,Qi, Qi,Gurry, Thomas,et al. Modular genetic design of multi-domain functional amyloids: insights into self-assembly and functional properties[J]. CHEMICAL SCIENCE,2019,10(14):4004-4014. |
| APA | Cui, Mengkui.,Qi, Qi.,Gurry, Thomas.,Zhao, Tianxin.,An, Bolin.,...&Zhong, Chao.(2019).Modular genetic design of multi-domain functional amyloids: insights into self-assembly and functional properties.CHEMICAL SCIENCE,10(14),4004-4014. |
| MLA | Cui, Mengkui,et al."Modular genetic design of multi-domain functional amyloids: insights into self-assembly and functional properties".CHEMICAL SCIENCE 10.14(2019):4004-4014. |
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