Modular genetic design of multi-domain functional amyloids: insights into self-assembly and functional properties
2019-04-14
Source PublicationCHEMICAL SCIENCE
ISSN2041-6520
Volume10Issue:14Pages:4004-4014
Status已发表
DOI10.1039/c9sc00208a
AbstractEngineering functional amyloids through a modular genetic strategy represents new opportunities for creating multifunctional molecular materials with tailored structures and performance. Despite important advances, how fusion modules affect the self-assembly and functional properties of amyloids remains elusive. Here, using Escherichia coli curli as a model system, we systematically studied the effect of flanking domains on the structures, assembly kinetics and functions of amyloids. The designed amyloids were composed of E. coli biofilm protein CsgA (as amyloidogenic cores) and one or two flanking domains, consisting of chitin-binding domains (CBDs) from Bacillus circulans chitinase, and/or mussel foot proteins (Mfps). Incorporation of fusion domains did not disrupt the typical -sheet structures, but indeed affected assembly rate, morphology, and stiffness of resultant fibrils. Consequently, the CsgA-fusion fibrils, particularly those containing three domains, were much shorter than the CsgA-only fibrils. Furthermore, the stiffness of the resultant fibrils was heavily affected by the structural feature of fusion domains, with -sheet-containing domains tending to increase the Young's modulus while random coil domains decreasing the Young's modulus. In addition, fibrils containing CBD domains showed higher chitin-binding activity compared to their CBD-free counterparts. The CBD-CsgA-Mfp3 construct exhibited significantly lower binding activity than Mfp5-CsgA-CBD due to inappropriate folding of the CBD domain in the former construct, in agreement with results based upon molecular dynamics modeling. Our study provides new insights into the assembly and functional properties of designer amyloid proteins with increasing complex domain structures and lays the foundation for the future design of functional amyloid-based structures and molecular materials.
Indexed ByEI ; SCIE ; SCI
Language英语
Funding ProjectNational Natural Science Foundation of China[U1532127] ; National Natural Science Foundation of China[31570972]
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
WOS IDWOS:000467988600008
PublisherROYAL SOC CHEMISTRY
EI Accession Number20191506765710
EI KeywordsBacteriology ; Chitin ; Elastic moduli ; Escherichia coli ; Glycoproteins ; Molecular dynamics ; Proteins ; Self assembly ; Stiffness
EI Classification NumberStructural Design, General:408.1 ; Biology:461.9 ; Physical Chemistry:801.4 ; Organic Compounds:804.1 ; Materials Science:951
WOS KeywordEXTRACELLULAR-MATRIX ; STRUCTURAL PREDICTIONS ; MECHANICAL-PROPERTIES ; FIBRILS ; A-BETA-42 ; DETERMINANTS ; BIOGENESIS ; ADHESIVES ; PROTEINS
Original Document TypeArticle
Citation statistics
Document Type期刊论文
Identifierhttps://kms.shanghaitech.edu.cn/handle/2MSLDSTB/40852
Collection物质科学与技术学院_博士生
物质科学与技术学院_PI研究组_钟超组
物质科学与技术学院_硕士生
物质科学与技术学院_本科生
Corresponding AuthorZhong, Chao
Affiliation
1.ShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai 200120, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Organ Chem, Shanghai 200032, Peoples R China
4.MIT, Dept Biol Engn, 77 Massachusetts Ave, Cambridge, MA 02139 USA
5.Chinese Acad Sci, Shanghai Inst Organ Chem, Interdisciplinary Res Ctr Biol & Chem, Shanghai 200032, Peoples R China
6.MIT, Dept Elect Engn & Comp Sci, Dept Biol Engn, Cambridge, MA 02139 USA
7.Politecn Milan, Dipartimento Chim Mat & Ingn Chim G Natta, Piazza Leonardo Vinci 32, I-20133 Milan, Italy
8.Imperial Coll London, Dept Mat, London SW7 2AZ, England
First Author AffilicationSchool of Physical Science and Technology
Corresponding Author AffilicationSchool of Physical Science and Technology
First Signature AffilicationSchool of Physical Science and Technology
Recommended Citation
GB/T 7714
Cui, Mengkui,Qi, Qi,Gurry, Thomas,et al. Modular genetic design of multi-domain functional amyloids: insights into self-assembly and functional properties[J]. CHEMICAL SCIENCE,2019,10(14):4004-4014.
APA Cui, Mengkui.,Qi, Qi.,Gurry, Thomas.,Zhao, Tianxin.,An, Bolin.,...&Zhong, Chao.(2019).Modular genetic design of multi-domain functional amyloids: insights into self-assembly and functional properties.CHEMICAL SCIENCE,10(14),4004-4014.
MLA Cui, Mengkui,et al."Modular genetic design of multi-domain functional amyloids: insights into self-assembly and functional properties".CHEMICAL SCIENCE 10.14(2019):4004-4014.
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