A higher-yield hybrid rice is achieved by assimilating a dominant heterotic gene in inbred parental lines

doi:10.1111/pbi.14295

	A higher-yield hybrid rice is achieved by assimilating a dominant heterotic gene in inbred parental lines
	Changsheng Wang 1; Ziqun Wang 1; Yunxiao Cai1,2 ; Zhou Zhu 1; Danheng Yu 3; Lei Hong 1; Yongchun Wang 1; Wei Lv 1; Qiang Zhao 1; Lizhen Si 1; Kun Liu 1; Bin Han 1
	2024-03-07
发表期刊	PLANT BIOTECHNOLOGY JOURNAL (IF:10.1[JCR-2023],11.6[5-Year])
ISSN	1467-7652
发表状态	已发表
DOI	10.1111/pbi.14295
摘要	The exploitation of heterosis to integrate parental advantages is one of the fastest and most efficient ways of rice breeding. The genomic architecture of heterosis suggests that the grain yield is strongly correlated with the accumulation of numerous rare superior alleles with positive dominance. However, the improvements in yield of hybrid rice have shown a slowdown or even plateaued due to the limited availability of complementary superior alleles. In this study, we achieved a considerable increase in grain yield of restorer lines by inducing an alternative splicing event in a heterosis gene OsMADS1 through CRISPR-Cas9, which accounted for approximately 34.1%–47.5% of yield advantage over their corresponding inbred rice cultivars. To achieve a higher yield in hybrid rice, we crossed the gene-edited restorer parents harbouring OsMADS1GW3p6 with the sterile lines to develop new rice hybrids. In two-line hybrid rice Guangliang- you 676 (GLY676), the yield of modified hybrids carrying the homozygous heterosis gene OsMADS1GW3p6 significantly exceeded that of the original hybrids with heterozygous OsMADS1. Similarly, the gene-modified F1 hybrids with heterozygous OsMADS1GW3p6 increased grain yield by over 3.4% compared to the three-line hybrid rice Quan-you-si-miao (QYSM) with the homozygous genotype of OsMADS1. Our study highlighted the great potential in increasing the grain yield of hybrid rice by pyramiding a single heterosis gene via CRISPR-Cas9. Furthermore, these results demonstrated that the incomplete dominance of heterosis genes played a major role in yield-related heterosis and provided a promising strategy for breeding higher-yielding rice varieties above what is currently achievable.
关键词	heterosis crispr/cas9 rice breeding grian yield grain size
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/364624
专题	生命科学与技术学院生命科学与技术学院_博士生
共同第一作者	Ziqun Wang
通讯作者	Bin Han
作者单位	1.National Center for Gene Research, State Key Laboratory of Plant Molecular Genetics, Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China. 2.School of Life Science and Technology, ShanghaiTech University, Shanghai, China. 3.Department of Life Sciences, Imperial College London, South Kensington, London, UK.
推荐引用方式 GB/T 7714	Changsheng Wang,Ziqun Wang,Yunxiao Cai,et al. A higher-yield hybrid rice is achieved by assimilating a dominant heterotic gene in inbred parental lines[J]. PLANT BIOTECHNOLOGY JOURNAL,2024.
APA	Changsheng Wang.,Ziqun Wang.,Yunxiao Cai.,Zhou Zhu.,Danheng Yu.,...&Bin Han.(2024).A higher-yield hybrid rice is achieved by assimilating a dominant heterotic gene in inbred parental lines.PLANT BIOTECHNOLOGY JOURNAL.
MLA	Changsheng Wang,et al."A higher-yield hybrid rice is achieved by assimilating a dominant heterotic gene in inbred parental lines".PLANT BIOTECHNOLOGY JOURNAL (2024).