CASK and FARP coordinately control pre- and post-synaptic function

doi:10.1101/2022.04.22.489135

	CASK and FARP coordinately control pre- and post-synaptic function
	Li, L.1; Liu, H.1; Qian, K.-y.2 ; Nurrish, S.3,4; Zeng, X.-t.2 ; Kaplan, J. M.3,4,5; Tong, X.-j.2 ; Hu, Z.1
	2022-04-22
状态	已发表
摘要	Synaptic proteins are immobilized at synapses via interactions with scaffolding proteins. Scaffolding proteins for glutamatergic and GABAergic synapses have been extensively studied; however, far less is known about how scaffolds shape the function of cholinergic synapses. Here we analyze the role of two C. elegans post-synaptic scaffolding proteins (LIN-2/CASK and FRM-3/FARP) at cholinergic neuromuscular junctions. Constitutive knockouts or muscle specific inactivation of lin-2 and frm-3 dramatically reduced spontaneous and evoked post-synaptic currents. These synaptic defects resulted from decreased abundance of post-synaptic ionotropic acetylcholine receptors (AChRs) and decreased presynaptic ACh release. Thus, our findings show that post-synaptic LIN-2/FRM-3 complexes coordinately control pre- and post-synaptic function, thereby promoting cholinergic synaptic transmission.
语种	英语
DOI	10.1101/2022.04.22.489135
相关网址	查看原文
出处	bioRxiv
收录类别	PPRN.PPRN
WOS记录号	PPRN:9715216
WOS类目	Neurosciences
资助项目	National Health and Medical Research Council["GNT1122351","24980","1R21EY029450-01","19JC1414100"] ; NARSAD Young Investigator grant["NS32196","21ZR1481000"]
文献类型	预印本
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/348540
专题	生命科学与技术学院生命科学与技术学院_PI研究组_童夏静组生命科学与技术学院_博士生
通讯作者	Kaplan, J. M.; Tong, X.-j.; Hu, Z.
作者单位	1.Univ Queensland, Queensland Brain Inst, Clem Jones Ctr Ageing Dementia Res CJCADR, Brisbane, Australia 2.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 3.Massachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114, USA 4.Harvard Med Sch, Dept Neurobiol, Boston, MA 02115, USA 5.Harvard Med Sch, Program Neurosci, Bosto, MA 02115, USA
推荐引用方式 GB/T 7714	Li, L.,Liu, H.,Qian, K.-y.,et al. CASK and FARP coordinately control pre- and post-synaptic function. 2022.