CRISPR-based Live Imaging of Epigenetic Modification-Mediated Genome Reorganization

doi:10.1101/2020.02.18.954610

	CRISPR-based Live Imaging of Epigenetic Modification-Mediated Genome Reorganization
	Feng, Y.; Wang, Y.; Yang, C.; Naseri, A.; Pederson, T.; Zheng, J.; Xiao, X.; Zhang, S.; Xie, W.; Ma, H.
	2020-02-19
状态	已发表
摘要	Epigenetic modifications play an essential role in chromatin architecture and dynamics. The role of epigenetic modification in chromatin organization has been studied by Hi-C from population cells, but imaging techniques to study their correlation and regulation in single living cells are lacking. Here we develop a CRISPR-based EpiGo (Epigenetic perturbation induced Genome organization) system to track epigenetic modification-mediated relocation, interaction or reorganization of genomic regions in living cells. EpiGo-KRAB is sufficient to induce the relocation of genomic loci to HP1 condensates and trigger genomic interactions. EpiGo-KRAB also triggers the induction of H3K9me3 at large genomic regions, which decorate on the surface of HP1 condensates possibly driven by phase separation.
语种	英语
DOI	10.1101/2020.02.18.954610
相关网址	查看原文
出处	bioRxiv
收录类别	PPRN.PPRN
WOS记录号	PPRN:14445002
WOS类目	Biochemistry & Molecular Biology
文献类型	预印本
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/348527
专题	生命科学与技术学院
作者单位	School of Life Science and Technology, ShanghaiTech University, Shanghai, China.
推荐引用方式 GB/T 7714	Feng, Y.,Wang, Y.,Yang, C.,et al. CRISPR-based Live Imaging of Epigenetic Modification-Mediated Genome Reorganization. 2020.