| CRISPR-based Live Imaging of Epigenetic Modification-Mediated Genome Reorganization |
| Feng, Y.; Wang, Y.; Yang, C.; Naseri, A.; Pederson, T.; Zheng, J.; Xiao, X.; Zhang, S.; Xie, W.; Ma, H.
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| 2020-02-19
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| 状态 | 已发表
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| 摘要 | Epigenetic modifications play an essential role in chromatin architecture and dynamics. The role of epigenetic modification in chromatin organization has been studied by Hi-C from population cells, but imaging techniques to study their correlation and regulation in single living cells are lacking. Here we develop a CRISPR-based EpiGo (Epigenetic perturbation induced Genome organization) system to track epigenetic modification-mediated relocation, interaction or reorganization of genomic regions in living cells. EpiGo-KRAB is sufficient to induce the relocation of genomic loci to HP1 condensates and trigger genomic interactions. EpiGo-KRAB also triggers the induction of H3K9me3 at large genomic regions, which decorate on the surface of HP1 condensates possibly driven by phase separation. |
| 语种 | 英语
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| DOI | 10.1101/2020.02.18.954610
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| 相关网址 | 查看原文
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| 出处 | bioRxiv
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| 收录类别 | PPRN.PPRN
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| WOS记录号 | PPRN:14445002
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| WOS类目 | Biochemistry & Molecular Biology
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| 文献类型 | 预印本
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| 条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/348527
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| 专题 | 生命科学与技术学院
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| 作者单位 | School of Life Science and Technology, ShanghaiTech University, Shanghai, China.
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推荐引用方式
GB/T 7714 |
Feng, Y.,Wang, Y.,Yang, C.,et al. CRISPR-based Live Imaging of Epigenetic Modification-Mediated Genome Reorganization. 2020.
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