Super-resolution imaging reveals dynamic skeletal cytoophidia

doi:10.1101/2022.08.29.505636

	Super-resolution imaging reveals dynamic skeletal cytoophidia
	Fang, Y.1 ; Li, Y.-l.1; Li, X.-m.1 ; Liu, J.-l.1,2
	2022-08-29
状态	已发表
摘要	CTP synthase (CTPS) can form filamentous structures termed cytoophidia in cells from all three domains of life. In order to study the mesoscale structure of cytoophidia, we perform fluorescence recovery after photobleaching (FRAP) and stimulated emission depletion (STED) microscopy in human cells. By using EGFP dimeric tag, as a tool to explore the physical properties of cytoophidia, we find that cytoophidia are dynamic and skeletal. The skeletal structure of CTPS cytoophidia may provide space for other components such as IMPDH. In addition, we observe CTPS granules with tentacles.
关键词	CTP synthase cytoophidium fluorescence recovery after photobleaching (FRAP) Stimulated emission depletion (STED)
语种	英语
DOI	10.1101/2022.08.29.505636
相关网址	查看原文
出处	bioRxiv
收录类别	PPRN.PPRN
WOS记录号	PPRN:12677199
WOS类目	Biophysics
资助项目	Ministry of Science and Technology of China["MC_UU_12021/3","20JC1410500","31771490","2021YFA0804700"] ; National Natural Science Foundation of China[MC_U137788471]
文献类型	预印本
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/348520
专题	生命科学与技术学院生命科学与技术学院_PI研究组_刘冀珑组生命科学与技术学院_公共科研平台_分子影像平台生命科学与技术学院_硕士生
通讯作者	Liu, J.-l.
作者单位	1.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 2.Univ Oxford, Dept Physiol, Anat & Genet, Oxford OX13PT, Oxfordshire, England
推荐引用方式 GB/T 7714	Fang, Y.,Li, Y.-l.,Li, X.-m.,et al. Super-resolution imaging reveals dynamic skeletal cytoophidia. 2022.