A single-cell transcriptional landscape of immune cells shows disease-specific changes of T cell and macrophage populations in human achalasia

doi:10.1038/s41467-023-39750-5

	A single-cell transcriptional landscape of immune cells shows disease-specific changes of T cell and macrophage populations in human achalasia
	Liu, Zu-Qiang 1,2,3; Dai, Hao 4; Yao, Lu 1,2,3; Chen, Wei-Feng 1,2,3; Wang, Yun 1,2,3; Ma, Li-Yun 1,2,3; Li, Xiao-Qing 1,2,3; Lin, Sheng-Li 1,2,3; He, Meng-Jiang 1,2,3; Gao, Ping-Ting 1,2,3; Liu, Xin-Yang 1,2,3; Xu, Jia-Xin 1,2,3; Xu, Xiao-Yue 1,2,3; Wang, Ke-Hao 1,2,3; Wang, Li 1,2,3; Chen, Luonan2,4,5,6 ; Zhou, Ping-Hong 1,2,3; Li, Quan-Lin 1,2,3
	2023-08-04
发表期刊	NATURE COMMUNICATIONS
EISSN	2041-1723
卷号	14 期号:1
发表状态	已发表
DOI	10.1038/s41467-023-39750-5
摘要	["Achalasia is a rare motility disorder of the esophagus resulting from abnormal immune responses, but the immunologic mechanism is unclear. Here the authors use scRNA-seq of PBMC and esophageal lower sphincter tissue and find C1QC(+) macrophages and tissue-resident memory T cells with expanded compositions and altered transcriptional profiles in achalasia.","Achalasia is a rare motility disorder of the esophagus caused by the gradual degeneration of myenteric neurons. Immune-mediated ganglionitis has been proposed to underlie the loss of myenteric neurons. Here, we measure the immune cell transcriptional profile of paired lower esophageal sphincter (LES) tissue and blood samples in achalasia and controls using single-cell RNA sequencing (scRNA-seq). In achalasia, we identify a pattern of expanded immune cells and a specific transcriptional phenotype, especially in LES tissue. We show C1QC(+) macrophages and tissue-resident memory T cells (T-RM), especially ZNF683(+) CD8(+) T-RM and XCL1(+) CD4(+) T-RM, are significantly expanded and localized surrounding the myenteric plexus in the LES tissue of achalasia. C1QC(+) macrophages are transcriptionally similar to microglia of the central nervous system and have a neurodegenerative dysfunctional phenotype in achalasia. T-RM also expresses transcripts of dysregulated immune responses in achalasia. Moreover, inflammation increases with disease progression since immune cells are more activated in type I compared with type II achalasia. Thus, we profile the immune cell transcriptional landscape and identify C1QC(+) macrophages and T-RM as disease-associated immune cell subsets in achalasia."]
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收录类别	SCI
语种	英语
资助项目	Natural Science Foundation of China["82170555","82000507","62132015","81873552","81670483","32100508","31930022","12131020","T2341007"] ; National Key Ramp;D Program of China[2022YFA1004800] ; Shanghai Rising-Star Program[19QA1401900] ; Yangfan Program of Shanghai Municipal Science and Technology Committee[20YF1406400] ; Major Project of Shanghai Municipal Science and Technology Committee["18ZR1406700","19441905200"] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB38040400] ; JST Moonshot Ramp;D[JPMJMS2021]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:001042606700009
出版者	NATURE PORTFOLIO
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/325786
专题	生命科学与技术学院生命科学与技术学院_特聘教授组_陈洛南组
通讯作者	Chen, Luonan; Zhou, Ping-Hong; Li, Quan-Lin
作者单位	1.Fudan Univ, Zhongshan Hosp, Endoscopy Ctr, Shanghai, Peoples R China 2.Fudan Univ, Zhongshan Hosp, Endoscopy Res Inst, Shanghai, Peoples R China 3.Shanghai Collaborat Innovat Ctr Endoscopy, Shanghai, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Ctr Excellence Mol Cell Sci, Key Lab Syst Biol, Shanghai, Peoples R China 5.Chinese Acad Sci, Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Key Lab Syst Hlth Sci Zhejiang Prov, Hangzhou, Peoples R China 6.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Liu, Zu-Qiang,Dai, Hao,Yao, Lu,et al. A single-cell transcriptional landscape of immune cells shows disease-specific changes of T cell and macrophage populations in human achalasia[J]. NATURE COMMUNICATIONS,2023,14(1).
APA	Liu, Zu-Qiang.,Dai, Hao.,Yao, Lu.,Chen, Wei-Feng.,Wang, Yun.,...&Li, Quan-Lin.(2023).A single-cell transcriptional landscape of immune cells shows disease-specific changes of T cell and macrophage populations in human achalasia.NATURE COMMUNICATIONS,14(1).
MLA	Liu, Zu-Qiang,et al."A single-cell transcriptional landscape of immune cells shows disease-specific changes of T cell and macrophage populations in human achalasia".NATURE COMMUNICATIONS 14.1(2023).