Dual-FRET imaging of IP3 and Ca2+ revealed Ca2+-induced IP3 production maintains long lasting Ca2+ oscillations in fertilized mouse eggs

doi:10.1038/s41598-019-40931-w

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	Dual-FRET imaging of IP3 and Ca2+ revealed Ca2+-induced IP3 production maintains long lasting Ca2+ oscillations in fertilized mouse eggs
	Matsu-ura, Toru 1; Shirakawa, Hideki 2; Suzuki, Kenichi G. N.3; Miyamoto, Akitoshi 1,4; Sugiura, Kotomi 5; Michikawa, Takayuki 6; Kusumi, Akihiro 7; Mikoshiba, Katsuhiko1,8,9
	2019-03-18
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	9
发表状态	已发表
DOI	10.1038/s41598-019-40931-w
摘要	In most species, fertilization induces Ca2+ transients in the egg. In mammals, the Ca2+ rises are triggered by phospholipase C zeta (PLC zeta) released from the sperm; IP3 generated by PLC zeta induces Ca2+ release from the intracellular Ca2+ store through IP3 receptor, termed IP3-induced Ca2+ release. Here, we developed new fluorescent IP3 sensors (IRIS-2s) with the wider dynamic range and higher sensitivity (Kd = 0.047-1.7 mu M) than that we developed previously. IRIS-2s employed green fluorescent protein and Halo-protein conjugated with the tetramethylrhodamine ligand as fluorescence resonance energy transfer (FRET) donor and acceptor, respectively. For simultaneous imaging of Ca2+ and IP3, using IRIS-2s as the IP3 sensor, we developed a new single fluorophore Ca2+ sensor protein, DYC3.60. With IRIS-2s and DYC3.60, we found that, right after fertilization, IP3 concentration ([IP3]) starts to increase before the onset of the first Ca2+ wave. [IP3] stayed at the elevated level with small peaks followed after Ca2+ spikes through Ca2+ oscillations. We detected delays in the peak of [IP3] compared to the peak of each Ca2+ spike, suggesting that Ca2+-induced regenerative IP3 production through PLC produces small [IP3] rises to maintain [IP3] over the basal level, which results in long lasting Ca2+ oscillations in fertilized eggs.
收录类别	SCI ; SCIE
语种	英语
资助项目	Ministry of Education, Science, Sports and Culture of Japan[22770227] ; Ministry of Education, Science, Sports and Culture of Japan[20370054] ; Ministry of Education, Science, Sports and Culture of Japan[2022007]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000461419200010
出版者	NATURE PUBLISHING GROUP
WOS关键词	INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR ; PHOSPHOLIPASE-C-ZETA ; CALCIUM OSCILLATIONS ; INTRACELLULAR CALCIUM ; SPERM ; ACTIVATION ; DYNAMICS ; RELEASE ; WAVES ; IDENTIFICATION
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/30571
专题	免疫化学研究所免疫化学研究所_PI研究组_Katsuhiko Mikoshiba组
通讯作者	Mikoshiba, Katsuhiko
作者单位	1.RIKEN, Ctr Brain Sci, Lab Dev Neurobiol, 2-1 Hirosawa, Wako, Saitama 3510198, Japan 2.Univ Electrocommun, Dept Appl Phys & Chem, Tokyo 1828585, Japan 3.Gifu Univ, Ctr Highly Adv Integrat Nano & Life Sci G CHAIN, 1-1 Yanagido, Gifu 5011193, Japan 4.Kyoto Univ, Grad Sch Biostudies, Lab Single Mol Cell Biol, Sakyo Ku, Konoe Cho, Kyoto 6068501, Japan 5.Univ Tokyo, Inst Med Sci, Int Res & Dev Ctr Mucosal Vaccine, Div Mucosal Vaccines,Minato Ku, 6-1 Shirokanedai, Tokyo 1088639, Japan 6.RIKEN, Ctr Adv Photon, Lab Biotechnol Opt Res, 2-1 Hirosawa, Wako, Saitama 3510198, Japan 7.Grad Univ, Okinawa Inst Sci & Technol, Onna, Okinawa 9040495, Japan 8.Keio Univ, Sch Med, Dept Pharmacol, Shnjuku Ku, 35 Shinanomachi, Tokyo 1608582, Japan 9.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai 201210, Peoples R China
通讯作者单位	免疫化学研究所
推荐引用方式 GB/T 7714	Matsu-ura, Toru,Shirakawa, Hideki,Suzuki, Kenichi G. N.,et al. Dual-FRET imaging of IP3 and Ca2+ revealed Ca2+-induced IP3 production maintains long lasting Ca2+ oscillations in fertilized mouse eggs[J]. SCIENTIFIC REPORTS,2019,9.
APA	Matsu-ura, Toru.,Shirakawa, Hideki.,Suzuki, Kenichi G. N..,Miyamoto, Akitoshi.,Sugiura, Kotomi.,...&Mikoshiba, Katsuhiko.(2019).Dual-FRET imaging of IP3 and Ca2+ revealed Ca2+-induced IP3 production maintains long lasting Ca2+ oscillations in fertilized mouse eggs.SCIENTIFIC REPORTS,9.
MLA	Matsu-ura, Toru,et al."Dual-FRET imaging of IP3 and Ca2+ revealed Ca2+-induced IP3 production maintains long lasting Ca2+ oscillations in fertilized mouse eggs".SCIENTIFIC REPORTS 9(2019).