Fsh-Pc-Sce complex mediates active transcription of Cubitus interruptus (Ci)

doi:10.1093/jmcb/mjy008

	Fsh-Pc-Sce complex mediates active transcription of Cubitus interruptus (Ci)
	Lv, Xiangdong 1; Chen, Hao 1; Zhang, Shuo 1; Zhang, Zhao 1; Pan, Chenyu 1; Xia, Yuanxin 1; Fan, Jialin 1; Wu, Wenqing 1; Lu, Yi 1; Zhang, Lei1,2 ; Wu, Hailong 1; Zhao, Yun1,2
	2018-10
发表期刊	JOURNAL OF MOLECULAR CELL BIOLOGY
ISSN	1674-2788
卷号	10 期号:5 页码:437-447
发表状态	已发表
DOI	10.1093/jmcb/mjy008
摘要	The Hedgehog (Hh) signaling pathway plays important roles in both embryonic development and adult tissue homeostasis. Such biological functions are mediated by the transcription factor Cubitus interruptus (Ci). Yet the transcriptional regulation of the effector Ci itself is poorly investigated. Through an RNAi-based genetic screen, we identified that female sterile (1) homeotic (Fsh), a transcription co-activator, directly activates Ci transcription. Biochemistry assays demonstrated physical interactions among Fsh, Sex combs extra (Sce), and Polycomb (Pc). Functional assays further showed that both Pc and Sce are required for Ci expression, which is not likely mediated by the derepression of Engrailed (En), a repressor of Ci, in Pc or Sce mutant cells. Finally, we provide evidence showing that Pc/Sce facilitates the binding of Fsh at Ci locus and that the physical interaction between Fsh and Pc is essential for Fsh-mediated Ci transcription. Taken together, we not only uncover that Ci is transcriptionally regulated by Fsh-Pc-Sce complex but also provide evidence for the coordination between Fsh and PcG proteins in transcriptional regulation.
关键词	Ci Fsh Hedgehog polycomb complex Sce transcriptional activation
收录类别	SCI ; SCIE ; CSCD
语种	英语
资助项目	'Cross and Cooperation in Science and Technology Innovation Team' Project of the Chinese Academy of Sciences[173176001000163307]
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000455315700007
CSCD记录号	CSCD:6375106
出版者	OXFORD UNIV PRESS
WOS关键词	EFFECT HOMEOTIC GENE ; HEDGEHOG PATHWAY ; STEM-CELLS ; POLYCOMB ; DROSOPHILA ; PROTEIN ; CHROMATIN ; MAINTENANCE ; HOMEOSTASIS ; ACTIVATION
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/30102
专题	生命科学与技术学院_特聘教授组_赵允组生命科学与技术学院_特聘教授组_张雷组
通讯作者	Wu, Hailong; Zhao, Yun
作者单位	1.Univ Chinese Acad Sci, State Key Lab Cell Biol, CAS Ctr Excellence Mol Cell Sci,Chinese Acad Sci, Innovat Ctr Cell Signaling Network,Shanghai Inst, Shanghai 200031, Peoples R China 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Lv, Xiangdong,Chen, Hao,Zhang, Shuo,et al. Fsh-Pc-Sce complex mediates active transcription of Cubitus interruptus (Ci)[J]. JOURNAL OF MOLECULAR CELL BIOLOGY,2018,10(5):437-447.
APA	Lv, Xiangdong.,Chen, Hao.,Zhang, Shuo.,Zhang, Zhao.,Pan, Chenyu.,...&Zhao, Yun.(2018).Fsh-Pc-Sce complex mediates active transcription of Cubitus interruptus (Ci).JOURNAL OF MOLECULAR CELL BIOLOGY,10(5),437-447.
MLA	Lv, Xiangdong,et al."Fsh-Pc-Sce complex mediates active transcription of Cubitus interruptus (Ci)".JOURNAL OF MOLECULAR CELL BIOLOGY 10.5(2018):437-447.