Intermittent protein restriction improves glucose homeostasis in Zucker diabetic fatty rats and single-cell sequencing reveals distinct changes in β cells

doi:10.1016/j.jnutbio.2023.109275

	Intermittent protein restriction improves glucose homeostasis in Zucker diabetic fatty rats and single-cell sequencing reveals distinct changes in β cells
	Li, Chenchen1 ; Zhu, Jing 2; Wei, Siying 2; Ye, Xiaoyi 2; Yang, Lanzexin 2; Wang, Zinan 2; Chen, Yan 2,3
	2023-04-01
发表期刊	JOURNAL OF NUTRITIONAL BIOCHEMISTRY; (IF:4.8[JCR-2023],5.4[5-Year])
ISSN	0955-2863
EISSN	1873-4847
卷号	114
发表状态	已发表
DOI	10.1016/j.jnutbio.2023.109275
摘要	Diabetes is caused by the interplay between genetic and environmental factors, therefore changes of lifestyle and dietary patterns are the most common practices for diabetes intervention. Protein restriction and caloric restriction have been shown to improve diabetic hyperglycemia in both animal models and humans. We report here the effectiveness of intermittent protein restriction (IPR) for the intervention of diabetes in Zucker diabetic fatty (ZDF) rats. Administration of IPR significantly reduced hyperglycemia and decreased glucose production in the liver. IPR protected pancreatic islets from diabetes-mediated damages as well as elevated the number and the proliferation activity of β cells. Single-cell RNA sequencing performed with isolated islets from the ZDF rats revealed that IPR was able to reverse the diabetes-associated β cell dedifferentiation. In addition, diabetic β cells in ZDF rats were associated with increased expressions of islet amyloid polypeptide, chromogranin and genes involved in endoplasmic reticulum stress. A β cell dedifferentiation marker Cd81 was also increased in the β cells of diabetic rats. In contrast, the expressions of D-box binding PAR bZIP transcription factor Dbp and immediate-early response genes were reduced in the diabetic β cells. In conclusion, these results indicated that IPR is effective in glycemic control and β cell protection in a diabetic rat model. In addition, diabetes in ZDF rats is associated with changes in the expression of genes involved in many facets of β cell functions.
关键词	Islets Protein restriction scRNA-seq Type 2 diabetes ZDF rats
学科门类	Endocrinology, Diabetes and Metabolism ; Biochemistry ; Molecular Biology ; Nutrition and Dietetics ; Clinical Biochemistry
URL	查看原文
收录类别	SCOPUS ; SCI
语种	英语
资助项目	National Natural Science Foundation of China[32230047]
WOS研究方向	Biochemistry & Molecular Biology ; Nutrition & Dietetics
WOS类目	Biochemistry & Molecular Biology ; Nutrition & Dietetics
WOS记录号	WOS:000964303200001
出版者	ELSEVIER SCIENCE INC
原始文献类型	Article
Scopus 记录号	2-s2.0-85147371126
来源库	SCOPUS
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/286466
专题	生命科学与技术学院生命科学与技术学院_博士生
通讯作者	Chen, Yan
作者单位	1.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 2.Univ Chinese Acad Sci, Shanghai Inst Nutr & Hlth, Chinese Acad Sci, CAS Key Lab Nutr Metab & Food Safety, Shanghai, Peoples R China 3.320 Yueyang Rd, New Life Sci Bld A2214, Shanghai 200031, Peoples R China
第一作者单位	生命科学与技术学院
第一作者的第一单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Li, Chenchen,Zhu, Jing,Wei, Siying,et al. Intermittent protein restriction improves glucose homeostasis in Zucker diabetic fatty rats and single-cell sequencing reveals distinct changes in β cells[J]. JOURNAL OF NUTRITIONAL BIOCHEMISTRY;,2023,114.
APA	Li, Chenchen.,Zhu, Jing.,Wei, Siying.,Ye, Xiaoyi.,Yang, Lanzexin.,...&Chen, Yan.(2023).Intermittent protein restriction improves glucose homeostasis in Zucker diabetic fatty rats and single-cell sequencing reveals distinct changes in β cells.JOURNAL OF NUTRITIONAL BIOCHEMISTRY;,114.
MLA	Li, Chenchen,et al."Intermittent protein restriction improves glucose homeostasis in Zucker diabetic fatty rats and single-cell sequencing reveals distinct changes in β cells".JOURNAL OF NUTRITIONAL BIOCHEMISTRY; 114(2023).