Real-Time Imaging of Endocytosis and Intracellular Trafficking of Semiconducting Polymer Dots

doi:10.1021/acsami.7b05662

	Real-Time Imaging of Endocytosis and Intracellular Trafficking of Semiconducting Polymer Dots
	Han, Yuping 1; Li, Xiaoming5 ; Chen, Haobin 4; Hu, Xingjie 2,3; Luo, Yao 1,2,3; Wang, Ting 1,2,3; Wang, Zejun 2,3; Li, Qian 2,3; Fan, Chunhai2,3,5 ; Shi, Jiye 2,3,6; Wang, Lihua 2,3; Zhao, Yun 1; Wu, Changfeng 4; Chen, Nan 2,3
	2017-06-28
发表期刊	ACS APPLIED MATERIALS & INTERFACES (IF:8.3[JCR-2023],8.7[5-Year])
ISSN	1944-8244
卷号	9 期号:25 页码:21200-21208
发表状态	已发表
DOI	10.1021/acsami.7b05662
摘要	Semiconducting polymer dots (Pdots) have shown great promise in biomedical applications, including biosensing, drug delivery, and live imaging of cells and biomolecules. Insight into the mechanism and regulation of cellular uptake and intracellular metabolism. of Pdots is important for the development of superior Pdots-based theranostic nanoconjugates. Herein, we performed real-time imaging of endocytosis and intracellular trafficking of a type of fluorescent Pdots that showed excellent biocompatibility in various types of cells. The endocytic routes and kinetics of Pdots were differently regulated in distinct cell types. Following endocytosis, Pdots were transported in vesicles along micro tubule and destined for lysosomes. Furthermore, our results revealed exosome-mediated extracellular release of Pdots and have tracked the dynamic process at the single particle level. These results provide new insight into the design of more effective and selective imaging probes as well as drug carriers.
关键词	fluorescent polymer dots endocytosis intracellular trafficking theranostic nanoparticles live cell imaging
收录类别	EI ; SCI
语种	英语
资助项目	Key Research Program of Frontier Sciences, CAS[QYZDJ-SSW-SLH031] ; Key Research Program of Frontier Sciences, CAS[QYZDJ-SSW-SLH019]
WOS研究方向	Science & Technology - Other Topics ; Materials Science
WOS类目	Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS记录号	WOS:000404807200017
出版者	AMER CHEMICAL SOC
EI入藏号	20172703879392
EI主题词	Biocompatibility ; Cells ; Cytology ; Fluorescence ; Functional polymers ; Medical applications ; Molecular biology ; Polymers
EI分类号	Bioengineering and Biology:461 ; Light/Optics:741.1 ; Polymeric Materials:815.1
WOS关键词	QUANTUM DOTS ; CELLULAR UPTAKE ; DRUG-DELIVERY ; LIVING CELLS ; LIVE CELLS ; NANOPARTICLES ; CYTOTOXICITY ; FLUORESCENCE ; TRANSDUCER ; INSIGHTS
原始文献类型	Article
通讯作者	Zhao, Yun ; Wu, Changfeng ; Chen, Nan
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2823
专题	生命科学与技术学院_公共科研平台_分子影像平台生命科学与技术学院_特聘教授组_樊春海组
通讯作者	Zhao, Yun; Wu, Changfeng; Chen, Nan
作者单位	1.Sichuan Univ, Coll Life Sci, Chengdu 610064, Peoples R China 2.Chinese Acad Sci, Div Phys Biol, Shanghai Synchrotron Radiat Facil, CAS Key Lab Interfacial Phys & Technol,Shanghai I, Shanghai 201800, Peoples R China 3.Chinese Acad Sci, Bioimaging Ctr, Shanghai Synchrotron Radiat Facil, CAS Key Lab Interfacial Phys & Technol,Shanghai I, Shanghai 201800, Peoples R China 4.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Guangdong, Peoples R China 5.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 6.UCB Pharma, 208 Bath Rd, Slough SL1 3WE, Berks, England
推荐引用方式 GB/T 7714	Han, Yuping,Li, Xiaoming,Chen, Haobin,et al. Real-Time Imaging of Endocytosis and Intracellular Trafficking of Semiconducting Polymer Dots[J]. ACS APPLIED MATERIALS & INTERFACES,2017,9(25):21200-21208.
APA	Han, Yuping.,Li, Xiaoming.,Chen, Haobin.,Hu, Xingjie.,Luo, Yao.,...&Chen, Nan.(2017).Real-Time Imaging of Endocytosis and Intracellular Trafficking of Semiconducting Polymer Dots.ACS APPLIED MATERIALS & INTERFACES,9(25),21200-21208.
MLA	Han, Yuping,et al."Real-Time Imaging of Endocytosis and Intracellular Trafficking of Semiconducting Polymer Dots".ACS APPLIED MATERIALS & INTERFACES 9.25(2017):21200-21208.