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Genetically engineered probiotics as catalytic glucose depriver for tumor starvation therapy | |
2023-02 | |
发表期刊 | MATERIALS TODAY BIO (IF:8.7[JCR-2023],8.8[5-Year]) |
ISSN | 2590-0064 |
EISSN | 2590-0064 |
卷号 | 18 |
发表状态 | 已发表 |
DOI | 10.1016/j.mtbio.2022.100515 |
摘要 | Cancer cells predominantly adapt the frequent but less efficient glycolytic process to produce ATPs rather than the highly efficient oxidative phosphorylation pathway. Such a regulated metabolic pattern in cancer cells offers promising therapeutic opportunities to kill tumors by glucose depletion or glycolysis blockade. In addition, to guarantee tumor-specific therapeutic targets, effective tumor-homing, accumulation, and retention strategies toward tumor regions should be elaborately designed. In the present work, genetically engineered tumor-targeting microbes (transgenic microorganism EcM-GDH (Escherichia coli MG1655) expressing exogenous glucose dehydrogenase (GDH) have been constructed to competitively deprive tumors of glucose nutrition for metabolic intervention and starvation therapy. Our results show that the engineered EcM-GDH can effectively deplete glucose and trigger pro-death autophagy and p53-initiated apoptosis in colorectal tumor cells/tissues both in vitro and in vivo. The present design illuminates the promising prospects for genetically engineered microbes in metabolic intervention therapeutics against malignant tumors based on catalytically nutrient deprivation, establishing an attractive probiotic therapeutic strategy with high effectiveness and biocompatibility. © 2022 The Authors |
关键词 | Biocompatibility Cell death Diseases Escherichia coli Genetic engineering Glucose Metabolism Probiotics Autophagy Cancer cells Catalytic glucose Glucose dehydrogenase Glucose deprivation Metabolic intervention Oxidative phosphorylation Probiotics Programmable living biomaterial Tumor-targeting |
URL | 查看原文 |
收录类别 | SCI ; EI ; SCOPUS |
语种 | 英语 |
资助项目 | National Natural Science Foundation of China[ |
WOS研究方向 | Engineering ; Materials Science |
WOS类目 | Engineering, Biomedical ; Materials Science, Biomaterials |
WOS记录号 | WOS:000978642000001 |
出版者 | Elsevier B.V. |
EI入藏号 | 20225113271539 |
EI主题词 | Tumors |
EI分类号 | 461.2 Biological Materials and Tissue Engineering ; 461.8.1 Genetic Engineering ; 461.9 Biology ; 461.9.1 Immunology ; 804.1 Organic Compounds |
原始文献类型 | Journal article (JA) |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/281944 |
专题 | 物质科学与技术学院 物质科学与技术学院_博士生 |
通讯作者 | Huo, Minfeng |
作者单位 | 1.Tongji Univ, Shanghai Peoples Hosp 10, Shanghai Frontiers Sci Ctr Nanocatalyt Med, Sch Med, Shanghai 200072, Peoples R China 2.Chinese Acad Sci, Chinese Acad Med Sci 2021RU012, Res Unit Nanocatalyt Med Speci Therapy Serious Dis, State Key Lab High Performance Ceram & Super fine, Shanghai 200050, Peoples R China 3.Univ Chinese Acad Sci, Ctr Mat Sci & Optoelect Engn, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, Ctr Mat Synthet Biol, Shenzhen 518055, Peoples R China 5.Chinese Acad Sci, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, Mat Synthet Biol Ctr,CAS Key Lab Quantitat Engn Bi, Shenzhen 518055, Peoples R China 6.Shanghai Tech Univ, Sch Phys Sci & Technol, Shanghai 201210, Peoples R China 7.Tongji Univ, Translat Res Inst Brain & Brain Like Intelligence, Shanghai Peoples Hosp 4, Sch Med, Shanghai 200081, Peoples R China |
推荐引用方式 GB/T 7714 | Ji, Penghao,An, Bolin,Jie, Zhongming,et al. Genetically engineered probiotics as catalytic glucose depriver for tumor starvation therapy[J]. MATERIALS TODAY BIO,2023,18. |
APA | Ji, Penghao.,An, Bolin.,Jie, Zhongming.,Wang, Liping.,Qiu, Shuwen.,...&Huo, Minfeng.(2023).Genetically engineered probiotics as catalytic glucose depriver for tumor starvation therapy.MATERIALS TODAY BIO,18. |
MLA | Ji, Penghao,et al."Genetically engineered probiotics as catalytic glucose depriver for tumor starvation therapy".MATERIALS TODAY BIO 18(2023). |
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