Genetically engineered probiotics as catalytic glucose depriver for tumor starvation therapy
2023-02
发表期刊MATERIALS TODAY BIO (IF:8.7[JCR-2023],8.8[5-Year])
ISSN2590-0064
EISSN2590-0064
卷号18
发表状态已发表
DOI10.1016/j.mtbio.2022.100515
摘要

Cancer cells predominantly adapt the frequent but less efficient glycolytic process to produce ATPs rather than the highly efficient oxidative phosphorylation pathway. Such a regulated metabolic pattern in cancer cells offers promising therapeutic opportunities to kill tumors by glucose depletion or glycolysis blockade. In addition, to guarantee tumor-specific therapeutic targets, effective tumor-homing, accumulation, and retention strategies toward tumor regions should be elaborately designed. In the present work, genetically engineered tumor-targeting microbes (transgenic microorganism EcM-GDH (Escherichia coli MG1655) expressing exogenous glucose dehydrogenase (GDH) have been constructed to competitively deprive tumors of glucose nutrition for metabolic intervention and starvation therapy. Our results show that the engineered EcM-GDH can effectively deplete glucose and trigger pro-death autophagy and p53-initiated apoptosis in colorectal tumor cells/tissues both in vitro and in vivo. The present design illuminates the promising prospects for genetically engineered microbes in metabolic intervention therapeutics against malignant tumors based on catalytically nutrient deprivation, establishing an attractive probiotic therapeutic strategy with high effectiveness and biocompatibility. © 2022 The Authors

关键词Biocompatibility Cell death Diseases Escherichia coli Genetic engineering Glucose Metabolism Probiotics Autophagy Cancer cells Catalytic glucose Glucose dehydrogenase Glucose deprivation Metabolic intervention Oxidative phosphorylation Probiotics Programmable living biomaterial Tumor-targeting
URL查看原文
收录类别SCI ; EI ; SCOPUS
语种英语
资助项目National Natural Science Foundation of China[
WOS研究方向Engineering ; Materials Science
WOS类目Engineering, Biomedical ; Materials Science, Biomaterials
WOS记录号WOS:000978642000001
出版者Elsevier B.V.
EI入藏号20225113271539
EI主题词Tumors
EI分类号461.2 Biological Materials and Tissue Engineering ; 461.8.1 Genetic Engineering ; 461.9 Biology ; 461.9.1 Immunology ; 804.1 Organic Compounds
原始文献类型Journal article (JA)
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/281944
专题物质科学与技术学院
物质科学与技术学院_博士生
通讯作者Huo, Minfeng
作者单位
1.Tongji Univ, Shanghai Peoples Hosp 10, Shanghai Frontiers Sci Ctr Nanocatalyt Med, Sch Med, Shanghai 200072, Peoples R China
2.Chinese Acad Sci, Chinese Acad Med Sci 2021RU012, Res Unit Nanocatalyt Med Speci Therapy Serious Dis, State Key Lab High Performance Ceram & Super fine, Shanghai 200050, Peoples R China
3.Univ Chinese Acad Sci, Ctr Mat Sci & Optoelect Engn, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, Ctr Mat Synthet Biol, Shenzhen 518055, Peoples R China
5.Chinese Acad Sci, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, Mat Synthet Biol Ctr,CAS Key Lab Quantitat Engn Bi, Shenzhen 518055, Peoples R China
6.Shanghai Tech Univ, Sch Phys Sci & Technol, Shanghai 201210, Peoples R China
7.Tongji Univ, Translat Res Inst Brain & Brain Like Intelligence, Shanghai Peoples Hosp 4, Sch Med, Shanghai 200081, Peoples R China
推荐引用方式
GB/T 7714
Ji, Penghao,An, Bolin,Jie, Zhongming,et al. Genetically engineered probiotics as catalytic glucose depriver for tumor starvation therapy[J]. MATERIALS TODAY BIO,2023,18.
APA Ji, Penghao.,An, Bolin.,Jie, Zhongming.,Wang, Liping.,Qiu, Shuwen.,...&Huo, Minfeng.(2023).Genetically engineered probiotics as catalytic glucose depriver for tumor starvation therapy.MATERIALS TODAY BIO,18.
MLA Ji, Penghao,et al."Genetically engineered probiotics as catalytic glucose depriver for tumor starvation therapy".MATERIALS TODAY BIO 18(2023).
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
个性服务
查看访问统计
谷歌学术
谷歌学术中相似的文章
[Ji, Penghao]的文章
[An, Bolin]的文章
[Jie, Zhongming]的文章
百度学术
百度学术中相似的文章
[Ji, Penghao]的文章
[An, Bolin]的文章
[Jie, Zhongming]的文章
必应学术
必应学术中相似的文章
[Ji, Penghao]的文章
[An, Bolin]的文章
[Jie, Zhongming]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。