Real-time observation of nucleoplasmin-mediated DNA decondensation and condensation reveals its specific functions as a chaperone

doi:10.1016/j.bbagrm.2018.07.002

	Real-time observation of nucleoplasmin-mediated DNA decondensation and condensation reveals its specific functions as a chaperone
	Huo, Xin-Mei 1; Meng, Li-feng 1; Jiang, Tao 2; Li, Ming 3; Sun, Fang-Zhen 2; Sun, Bo4 ; Li, Jian-Ke 1
	2018-08
发表期刊	BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS (IF:2.6[JCR-2023],3.6[5-Year])
ISSN	1874-9399
卷号	1861 期号:8 页码:743-751
发表状态	已发表
DOI	10.1016/j.bbagrm.2018.07.002
摘要	Fertilization requires decondensation of promatine-condensed sperm chromatin, a dynamic process serving as an attractive system for the study of chromatin reprogramming. Nucleoplasmin is a key factor in regulating nucleosome assembly as a chaperone during fertilization process. However, knowledge on nucleoplasmin in chromatin formation remains elusive. Herein, magnetic tweezers (MT) and a chromatin assembly system were used to study the nucleoplasmin-mediated DNA decondensation/condensation at the single-molecular level in vitro. We found that protamine induces DNA condensation in a stepwise manner. Once DNA was condensed, nucleoplasmin, polyglutamic acid, and RNA could remove protamine from the DNA at different rates. The affinity binding of the different polyanions with protamine suggests chaperone-mediated chromatin decondensation activity occurs through protein protein interactions. After decondensation, both RNA and polyglutamic acid prevented the transfer of histones onto the naked DNA. In contrast, nucleoplasmin is able to assist the histone transfer process, even though it carries the same negative charge as RNA and polyglutamic acid. These observations imply that the chaperone effects of nucleoplasmin during the decondensation/condensation process may be driven by specific spatial configuration of its acidic pentamer structure, rather than by electrostatic interaction. Our findings offer a novel molecular understanding of nucleoplasmin in sperm chromatin decondensation and subsequent developmental chromatin reprogramming at individual molecular level.
关键词	Nucleoplasmin Magnetic tweezers DNA condensation Decondensation Chromatin remodeling
收录类别	SCI ; SCIE
语种	英语
资助项目	earmarked fund for Modern Agro-Industry Technology Research System in China[CARS-44]
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS类目	Biochemistry & Molecular Biology ; Biophysics
WOS记录号	WOS:000441649200007
出版者	ELSEVIER SCIENCE BV
WOS关键词	XENOPUS-LAEVIS OOCYTES ; PHYSIOLOGICAL IONIC-STRENGTH ; ATOMIC-FORCE MICROSCOPY ; ASSEMBLY IN-VITRO ; EGG EXTRACTS ; SPERM DECONDENSATION ; HISTONE COMPLEXES ; NUCLEAR CHAPERONE ; ACIDIC PROTEIN ; CORE PARTICLE
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/27692
专题	生命科学与技术学院_PI研究组_孙博组
通讯作者	Sun, Bo; Li, Jian-Ke
作者单位	1.Chinese Acad Agr Sci, Inst Apicultural Res, Minist Agr, Key Lab Pollinating Insect Biol, Beijing 100081, Peoples R China 2.Chinese Acad Sci, Inst Genet & Dev Biol, Lab Mol Dev Biol, Beijing 100080, Peoples R China 3.Chinese Acad Sci, Inst Phys, Beijing 100190, Peoples R China 4.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Huo, Xin-Mei,Meng, Li-feng,Jiang, Tao,et al. Real-time observation of nucleoplasmin-mediated DNA decondensation and condensation reveals its specific functions as a chaperone[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS,2018,1861(8):743-751.
APA	Huo, Xin-Mei.,Meng, Li-feng.,Jiang, Tao.,Li, Ming.,Sun, Fang-Zhen.,...&Li, Jian-Ke.(2018).Real-time observation of nucleoplasmin-mediated DNA decondensation and condensation reveals its specific functions as a chaperone.BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS,1861(8),743-751.
MLA	Huo, Xin-Mei,et al."Real-time observation of nucleoplasmin-mediated DNA decondensation and condensation reveals its specific functions as a chaperone".BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS 1861.8(2018):743-751.