A fluorescence and UV/vis absorption dual-signaling probe with aggregation-induced emission characteristics for specific detection of cysteine

doi:10.1039/c8ra03756f

	A fluorescence and UV/vis absorption dual-signaling probe with aggregation-induced emission characteristics for specific detection of cysteine
	Li, Ruru1,3 ; Huang, Xiaoyu1,3 ; Lu, Guolin 1; Feng, Chun 1,2
	2018
发表期刊	RSC ADVANCES
ISSN	2046-2069
卷号	8 期号:43 页码:24346-24354
发表状态	已发表
DOI	10.1039/c8ra03756f
摘要	Biological thiols with similar structures, such as glutathione (GSH), N-acetyl-L-cysteine (NAC), homocysteine (Hcy) and cysteine (Cys), play important roles in human physiology and are associated with different diseases. Thus, the discrimination of these thiols is a great necessity for various biochemical investigations and the diagnosis of related diseases. Herein, we present a new dual-signaling probe consisting of a typical aggregation induced emission fluorogen of a tetraphenylethylene group and 2,4-dinitrobenzenesulfonyl moiety. The probe can be used to selectively and quantitatively detect Cys over a variety of bio-species, including GSH, NAC and Hcy, from both UV/vis absorption and fluorescence channels. The mechanism study showed that the fluorescence and UV/vis absorption were turned on as the probe undergoes displacement of the 2,4-dinitrobenzenesulfonyl group with Cys, where the UV/vis and fluorescence signals originate from the dinitrophenyl-containing compounds and aggregates of TPE-OH, respectively. In addition, the discrimination of Cys was achieved by more rapid intramolecular displacement of sulfur with the amino group of Cys than NAC, Hcy and GSH. Moreover, the probe shows ignorable cytotoxicity against HepG2 cells, which demonstrates the great potential of the probe in selectively detecting Cys in vivo.
收录类别	SCI ; SCIE ; EI
语种	英语
资助项目	State Key Laboratory of Molecular Engineering of Polymers[K2018-15]
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:000440243600030
出版者	ROYAL SOC CHEMISTRY
EI入藏号	20182905558170
EI主题词	Acetylcysteine ; Amino acids ; Diagnosis ; Fluorescence ; Physiology
EI分类号	Medicine and Pharmacology:461.6 ; Biology:461.9 ; Light/Optics:741.1 ; Organic Compounds:804.1
WOS关键词	SELECTIVE DETECTION ; LIVING CELLS ; LIGHT-UP ; GLUTATHIONE METABOLISM ; CU(II) IONS ; HOMOCYSTEINE ; SENSOR ; TETRAPHENYLETHYLENE ; CHEMODOSIMETER ; CHEMOSENSOR
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/27605
专题	物质科学与技术学院物质科学与技术学院_特聘教授组_黄晓宇组物质科学与技术学院_博士生
通讯作者	Huang, Xiaoyu; Feng, Chun
作者单位	1.Univ Chinese Acad Sci, Chinese Acad Sci, Ctr Excellence Mol Synth,Shanghai Inst Organ Chem, Key Lab Synthet & Self Assembly Chem Organ Funct, 345 Lingling Rd, Shanghai 200032, Peoples R China 2.Fudan Univ, Dept Macromol Sci, State Key Lab Mol Engn Polymers, 220 Handan Rd, Shanghai 200433, Peoples R China 3.ShanghaiTech Univ, Sch Phys Sci & Technol, 100 Haike Rd, Shanghai 201210, Peoples R China
第一作者单位	物质科学与技术学院
通讯作者单位	物质科学与技术学院
推荐引用方式 GB/T 7714	Li, Ruru,Huang, Xiaoyu,Lu, Guolin,et al. A fluorescence and UV/vis absorption dual-signaling probe with aggregation-induced emission characteristics for specific detection of cysteine[J]. RSC ADVANCES,2018,8(43):24346-24354.
APA	Li, Ruru,Huang, Xiaoyu,Lu, Guolin,&Feng, Chun.(2018).A fluorescence and UV/vis absorption dual-signaling probe with aggregation-induced emission characteristics for specific detection of cysteine.RSC ADVANCES,8(43),24346-24354.
MLA	Li, Ruru,et al."A fluorescence and UV/vis absorption dual-signaling probe with aggregation-induced emission characteristics for specific detection of cysteine".RSC ADVANCES 8.43(2018):24346-24354.