Somatosensory neurons express specific sets of lincRNAs, and lincRNA CLAP promotes itch sensation in mice

doi:10.15252/embr.202154313

	Somatosensory neurons express specific sets of lincRNAs, and lincRNA CLAP promotes itch sensation in mice
	Wang, Bin 1,2; Jiang, Bowen 1; Li, Guo-Wei 3; Dong, Fei 4,5; Luo, Zheng 3; Cai, Bing 2; Wei, Manyi 1; Huang, Jiansong 1; Wang, Kaikai2,6 ; Feng, Xin 1; Tong, Fang 7; Wang, Sashuang 2,8,9; Wang, Qiong 1; Han, Qingjian 7; Li, Changlin 2,10; Zhang, Xu2,4,5,6,10 ; Yang, Li 11,12; Bao, Lan1,6
	2022-12-01
发表期刊	EMBO REPORTS (IF:6.5[JCR-2023],8.3[5-Year])
ISSN	1469-221X
EISSN	1469-3178
发表状态	已发表
DOI	10.15252/embr.202154313
摘要	Somatosensory neurons are highly heterogeneous with distinct types of neural cells responding to specific stimuli. However, the distribution and roles of cell-type-specific long intergenic noncoding RNAs (lincRNAs) in somatosensory neurons remain largely unexplored. Here, by utilizing droplet-based single-cell RNA-seq (scRNA-seq) and full-length Smart-seq2, we show that lincRNAs, but not coding mRNAs, are enriched in specific types of mouse somatosensory neurons. Profiling of lincRNAs from single neurons located in dorsal root ganglia (DRG) identifies 200 lincRNAs localized in specific types or subtypes of somatosensory neurons. Among them, the conserved cell-type-specific lincRNA CLAP associates with pruritus and is abundantly expressed in somatostatin (SST)-positive neurons. CLAP knockdown reduces histamine-induced Ca2+ influx in cultured SST-positive neurons and in vivo reduces histamine-induced scratching in mice. In vivo knockdown of CLAP also decreases the expression of neuron-type-specific and itch-related genes in somatosensory neurons, and this partially depends on the RNA binding protein MSI2. Our data reveal a cell-type-specific landscape of lincRNAs and a function for CLAP in somatosensory neurons in sensory transmission.
关键词	cell-type-specific lincRNA itch sensation scRNA-seq somatosensory neuron
URL	查看原文
收录类别	SCI ; SCOPUS
语种	英语
资助项目	National Natural Science Foundation of China["32070968","32070967"] ; Guangdong High Level Innovation Research Institute[2021B0909050004] ; Innovation Fund for Medical Sciences of Chinese Academy of Medical Sciences[2019-12M-5-082]
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Cell Biology
WOS记录号	WOS:000899026700001
出版者	WILEY
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/272806
专题	生命科学与技术学院_博士生生命科学与技术学院_特聘教授组_鲍岚组生命科学与技术学院_特聘教授组_张旭组
通讯作者	Wang, Bin; Yang, Li; Bao, Lan
作者单位	1.Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, CAS Ctr Excellence Mol Cell Sci,State Key Lab Cell, Shanghai, Peoples R China 2.Guangdong Inst Intelligence Sci & Technol, Zhuhai, Peoples R China 3.Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai Inst Nutr & Hlth, CAS Key Lab Computat Biol, Shanghai, Peoples R China 4.Chinese Acad Sci, Ctr Excellence Brain Sci & Intelligence Technol, Inst Neurosci, Shanghai, Peoples R China 5.Chinese Acad Sci, Ctr Excellence Brain Sci & Intelligence Technol, State Key Lab Neurosci, Shanghai, Peoples R China 6.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 7.Fudan Univ, Inst Brain Sci, MOE Frontiers Ctr Brain Sci, State Key Lab Med Neurobiol, Shanghai, Peoples R China 8.Huazhong Univ Sci & Technol, Union Shenzhen Hosp, Dept Pain Med, Shenzhen, Peoples R China 9.Huazhong Univ Sci & Technol, Union Shenzhen Hosp, Shenzhen Municipal Key Lab Pain Med, Shenzhen, Peoples R China 10.Chinese Acad Sci, Chinese Acad Med Sci, Shanghai Adv Res Inst, Res Unit Pain, Shanghai, Peoples R China 11.Fudan Univ, Childrens Hosp, Ctr Mol Med, Shanghai, Peoples R China 12.Fudan Univ, Inst Biomed Sci, Shanghai Key Lab Med Epigenet, Int Lab Med Epigenet & Metab,Minist Sci & Technol, Shanghai, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Wang, Bin,Jiang, Bowen,Li, Guo-Wei,et al. Somatosensory neurons express specific sets of lincRNAs, and lincRNA CLAP promotes itch sensation in mice[J]. EMBO REPORTS,2022.
APA	Wang, Bin.,Jiang, Bowen.,Li, Guo-Wei.,Dong, Fei.,Luo, Zheng.,...&Bao, Lan.(2022).Somatosensory neurons express specific sets of lincRNAs, and lincRNA CLAP promotes itch sensation in mice.EMBO REPORTS.
MLA	Wang, Bin,et al."Somatosensory neurons express specific sets of lincRNAs, and lincRNA CLAP promotes itch sensation in mice".EMBO REPORTS (2022).