Biodegradation of highly crystallized poly(ethylene terephthalate) through cell surface codisplay of bacterial PETase and hydrophobin

doi:10.1038/s41467-022-34908-z

	Biodegradation of highly crystallized poly(ethylene terephthalate) through cell surface codisplay of bacterial PETase and hydrophobin
	Chen, Zhuozhi 1; Duan, Rongdi 1; Xiao, Yunjie 1; Wei, Yi 1; Zhang, Hanxiao 1; Sun, Xinzhao 1; Wang, Shen 1; Cheng, Yingying 1; Wang, Xue 1; Tong, Shanwei 1; Yao, Yunxiao 1; Zhu, Cheng 1; Yang, Haitao1,2,3,4 ; Wang, Yanyan 1; Wang, Zefang 1,2
	2022-11-21
发表期刊	NATURE COMMUNICATIONS
EISSN	2041-1723
卷号	13 期号:1
发表状态	已发表
DOI	10.1038/s41467-022-34908-z
摘要	The process of recycling poly(ethylene terephthalate) (PET) remains a major challenge due to the enzymatic degradation of high-crystallinity PET (hcPET). Recently, a bacterial PET-degrading enzyme, PETase, was found to have the ability to degrade the hcPET, but with low enzymatic activity. Here we present an engineered whole-cell biocatalyst to simulate both the adsorption and degradation steps in the enzymatic degradation process of PETase to achieve the efficient degradation of hcPET. Our data shows that the adhesive unit hydrophobin and degradation unit PETase are functionally displayed on the surface of yeast cells. The turnover rate of the whole-cell biocatalyst toward hcPET (crystallinity of 45%) dramatically increases approximately 328.8-fold compared with that of purified PETase at 30 °C. In addition, molecular dynamics simulations explain how the enhanced adhesion can promote the enzymatic degradation of PET. This study demonstrates engineering the whole-cell catalyst is an efficient strategy for biodegradation of PET.
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收录类别	SCIE ; SCI
语种	英语
资助项目	National Natural Science Foundation of China["82202518","22007071","22077094","31970048","61971302"]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000888056100015
出版者	NATURE PORTFOLIO
Scopus 记录号	2-s2.0-85142256975
来源库	Scopus
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/251411
专题	生命科学与技术学院免疫化学研究所_PI研究组_杨海涛组
通讯作者	Wang, Yanyan; Wang, Zefang
作者单位	1.Tianjin Univ, Coll Precis Instrument & Optoelect Engn, Sch Life Sci,Minist Educ,Key Lab Syst Bioengn, Tianjin Key Lab Funct & Applicat Biol Macromol St, Tianjin 300072, Peoples R China 2.Tianjin Int Joint Acad Biotechnol & Med, Tianjin 300457, Peoples R China 3.Shanghai Tech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai 201210, Peoples R China 4.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
推荐引用方式 GB/T 7714	Chen, Zhuozhi,Duan, Rongdi,Xiao, Yunjie,et al. Biodegradation of highly crystallized poly(ethylene terephthalate) through cell surface codisplay of bacterial PETase and hydrophobin[J]. NATURE COMMUNICATIONS,2022,13(1).
APA	Chen, Zhuozhi.,Duan, Rongdi.,Xiao, Yunjie.,Wei, Yi.,Zhang, Hanxiao.,...&Wang, Zefang.(2022).Biodegradation of highly crystallized poly(ethylene terephthalate) through cell surface codisplay of bacterial PETase and hydrophobin.NATURE COMMUNICATIONS,13(1).
MLA	Chen, Zhuozhi,et al."Biodegradation of highly crystallized poly(ethylene terephthalate) through cell surface codisplay of bacterial PETase and hydrophobin".NATURE COMMUNICATIONS 13.1(2022).