Identification of a rare Gli1+ progenitor cell population contributing to liver regeneration during chronic injury

doi:10.1038/s41421-022-00474-3

	Identification of a rare Gli1+ progenitor cell population contributing to liver regeneration during chronic injury
	Peng, Jiayin 1; Li, Fei 1; Wang, Jia 1,2; Wang, Chaoxiong 1,2; Jiang, Yiao 1,2; Liu, Biao 1,2; He, Juan 1,2; Yuan, Kai 1; Pan, Chenyu 3; Lin, Moubin 3; Zhou, Bin 1; Chen, Luonan 1; Gao, Dong 1,4; Zhao, Yun1,5,6
	2022-11-01
发表期刊	CELL DISCOVERY
ISSN	2056-5968
EISSN	2056-5968
卷号	8 期号:1
发表状态	已发表
DOI	10.1038/s41421-022-00474-3
摘要	In adults, hepatocytes are mainly replenished from the existing progenitor pools of hepatocytes and cholangiocytes during chronic liver injury. However, it is unclear whether other cell types in addition to classical hepatocytes and cholangiocytes contribute to hepatocyte regeneration after chronic liver injuries. Here, we identified a new biphenotypic cell population that contributes to hepatocyte regeneration during chronic liver injuries. We found that a cell population expressed Gli1 and EpCAM (EpCAMGli1), which was further characterized with both epithelial and mesenchymal identities by single-cell RNA sequencing. Genetic lineage tracing using dual recombinases revealed that Gli1 nonhepatocyte cell population could generate hepatocytes after chronic liver injury. EpCAMGli1 cells exhibited a greater capacity for organoid formation with functional hepatocytes in vitro and liver regeneration upon transplantation in vivo. Collectively, these findings demonstrate that EpCAMGli1 cells can serve as a new source of liver progenitor cells and contribute to liver repair and regeneration.
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收录类别	SCIE
语种	英语
Scopus 记录号	2-s2.0-85140989311
来源库	Scopus
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/248898
专题	生命科学与技术学院_特聘教授组_赵允组
通讯作者	Gao, Dong; Zhao, Yun
作者单位	1.State Key Laboratory of Molecular Biology,Shanghai Institute of Biochemistry and Cell Biology,Center for Excellence in Molecular Cell Science,Chinese Academy of Sciences,Shanghai,China 2.University of Chinese Academy of Sciences,Beijing,China 3.Department of General Surgery,Yangpu Hospital,Tongji University School of Medicine,Shanghai,China 4.Institute for Stem Cell and Regeneration,Chinese Academy of Sciences,Beijing,China 5.School of Life Science and Technology,ShanghaiTech University,Shanghai,China 6.Key Laboratory of Systems Health Science of Zhejiang Province,School of Life Science,Hangzhou Institute for Advanced Study,University of Chinese Academy of Sciences,Hangzhou,Zhejiang,China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Peng, Jiayin,Li, Fei,Wang, Jia,et al. Identification of a rare Gli1+ progenitor cell population contributing to liver regeneration during chronic injury[J]. CELL DISCOVERY,2022,8(1).
APA	Peng, Jiayin.,Li, Fei.,Wang, Jia.,Wang, Chaoxiong.,Jiang, Yiao.,...&Zhao, Yun.(2022).Identification of a rare Gli1+ progenitor cell population contributing to liver regeneration during chronic injury.CELL DISCOVERY,8(1).
MLA	Peng, Jiayin,et al."Identification of a rare Gli1+ progenitor cell population contributing to liver regeneration during chronic injury".CELL DISCOVERY 8.1(2022).