ShanghaiTech University Knowledge Management System
Observing the overall rocking motion of a protein in a crystal | |
2015 | |
发表期刊 | NATURE COMMUNICATIONS |
EISSN | 20411723 |
卷号 | 6 |
发表状态 | 已发表 |
DOI | 10.1038/ncomms9361 |
摘要 | The large majority of three-dimensional structures of biological macromolecules have been determined by X-ray diffraction of crystalline samples. High-resolution structure determination crucially depends on the homogeneity of the protein crystal. Overall 'rocking' motion of molecules in the crystal is expected to influence diffraction quality, and such motion may therefore affect the process of solving crystal structures. Yet, so far overall molecular motion has not directly been observed in protein crystals, and the timescale of such dynamics remains unclear. Here we use solid-state NMR, X-ray diffraction methods and 1/4s-long molecular dynamics simulations to directly characterize the rigid-body motion of a protein in different crystal forms. For ubiquitin crystals investigated in this study we determine the range of possible correlation times of rocking motion, 0.1-100 μs. The amplitude of rocking varies from one crystal form to another and is correlated with the resolution obtainable in X-ray diffraction experiments. © 2015 Macmillan Publishers Limited. All rights reserved. |
URL | 查看原文 |
收录类别 | EI |
语种 | 英语 |
出版者 | Nature Publishing Group, Houndmills, Basingstoke, Hampshire, RG21 6XS, United Kingdom |
EI入藏号 | 4768567 |
EI主题词 | crystal structure - crystallinity - data quality - homogeneity - molecular analysis - protein - three-dimensional modeling - X-ray diffraction |
EI分类号 | 901 Geobase: Related Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2485 |
专题 | 生命科学与技术学院_特聘教授组_James E. Rothman组 免疫化学研究所_特聘教授组_功能筛选实验室 |
作者单位 | 1.Université Grenoble Alpes, IBS, Grenoble, France; 2.CEA, Institut de Biologie Structurale, Grenoble, France; 3.CNRS, Institut de Biologie Structurale, Grenoble, France; 4.Department of Chemistry, Purdue University, West Lafayette; IN, United States; 5.Institut für Physikalische Biologie, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany; 6.ICS-6: Structural Biochemistry, Forschungszentrum Jülich, Jülich, Germany; 7.Laboratory of Biomolecular NMR, St. Petersburg State University, St.-Petersburg, Russia; 8.Shanghai Institute for Advanced Immunochemical Studies (SIAIS), ShanghaiTech University, Shanghai, China |
第一作者单位 | 免疫化学研究所 |
推荐引用方式 GB/T 7714 | Ma, Peixiang,Xue, Yi,Coquelle, Nicolas,et al. Observing the overall rocking motion of a protein in a crystal[J]. NATURE COMMUNICATIONS,2015,6. |
APA | Ma, Peixiang.,Xue, Yi.,Coquelle, Nicolas.,Haller, Jens D..,Yuwen, Tairan.,...&Schanda, Paul.(2015).Observing the overall rocking motion of a protein in a crystal.NATURE COMMUNICATIONS,6. |
MLA | Ma, Peixiang,et al."Observing the overall rocking motion of a protein in a crystal".NATURE COMMUNICATIONS 6(2015). |
条目包含的文件 | ||||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 |
修改评论
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。