CTCF DNA-binding domain undergoes dynamic and selective protein-protein interactions

doi:10.1016/j.isci.2022.105011

	CTCF DNA-binding domain undergoes dynamic and selective protein-protein interactions
	Zhou, Rong 1; Tian, Kai 1; Huang, Jie 1; Duan, Wenjia 1; Fu, Hongye 2; Feng, Ying3 ; Wang, Hui 1; Jiang, Yongpeng 1; Li, Yuanjun 1; Wang, Rui 1; Hu, Jiazhi 1; Ma, Hanhui3 ; Ji, Xiong 1; Qi, Zhi 2
	2022-09-16
发表期刊	ISCIENCE (IF:4.6[JCR-2023],5.0[5-Year])
EISSN	2589-0042
卷号	25 期号:9
发表状态	已发表
DOI	10.1016/j.isci.2022.105011
摘要	CTCF is a predominant insulator protein required for three-dimensional chro-matin organization. However, the roles of its insulation of enhancers in a 3D nu-clear organization have not been fully explained. Here, we found that the CTCF DNA-binding domain (DBD) forms dynamic self-interacting clusters. Strikingly, CTCF DBD clusters were found to incorporate other insulator proteins but are not coenriched with transcriptional activators in the nucleus. This property is not observed in other domains of CTCF or the DBDs of other transcription fac-tors. Moreover, endogenous CTCF shows a phenotype consistent with the DBD by forming small protein clusters and interacting with CTCF motif arrays that have fewer transcriptional activators bound. Our results reveal an interesting phenomenon in which CTCF DBD interacts with insulator proteins and selectively localizes to nuclear positions with lower concentrations of transcriptional activa-tors, providing insights into the insulation function of CTCF.
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收录类别	SCI ; SCIE
语种	英语
资助项目	National Natural Science Foundation of China["2017YFA0506600","31871309","32170569"] ; NSFC[31670762] ; China Postdoctoral Science Foundation[2017M610700]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000860969300003
出版者	CELL PRESS
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/238160
专题	生命科学与技术学院_PI研究组_马涵慧组
通讯作者	Ji, Xiong; Qi, Zhi
作者单位	1.Peking Univ, Peking Tsinghua Ctr Life Sci, Sch Life Sci, Key Lab Cell Proliferat, Beijing 100871, Peoples R China 2.Peking Univ, Acad Adv Interdisciplinary Studies, Ctr Quantitat Biol, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China 3.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 201210, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Rong,Tian, Kai,Huang, Jie,et al. CTCF DNA-binding domain undergoes dynamic and selective protein-protein interactions[J]. ISCIENCE,2022,25(9).
APA	Zhou, Rong.,Tian, Kai.,Huang, Jie.,Duan, Wenjia.,Fu, Hongye.,...&Qi, Zhi.(2022).CTCF DNA-binding domain undergoes dynamic and selective protein-protein interactions.ISCIENCE,25(9).
MLA	Zhou, Rong,et al."CTCF DNA-binding domain undergoes dynamic and selective protein-protein interactions".ISCIENCE 25.9(2022).