Quantitative Proteomics Explore the Potential Targets and Action Mechanisms of Hydroxychloroquine

doi:10.3390/molecules27165175

	Quantitative Proteomics Explore the Potential Targets and Action Mechanisms of Hydroxychloroquine
	Zhao, Jingxiang 1,2; Zhao, Zhiqiang2,3 ; Hou, Wanting 2; Jiang, Yue 2,4; Liu, Guobin 2; Ren, Xuelian 2; Liu, Kun 4; Liu, Hong 1,2,5; Chen, Kaixian 1,2,5; Huang, He 2,5
	2022-08
发表期刊	MOLECULES (IF:4.2[JCR-2023],4.6[5-Year])
EISSN	1420-3049
卷号	27 期号:16
发表状态	已发表
DOI	10.3390/molecules27165175
摘要	Hydroxychloroquine (HCQ) is an autophagy inhibitor that has been used for the treatment of many diseases, such as malaria, rheumatoid arthritis, systemic lupus erythematosus, and cancer. Despite the therapeutic advances in these diseases, the underlying mechanisms have not been well determined and hinder the rational use of this drug in the future. Here, we explored the possible mechanisms and identified the potential binding targets of HCQ by performing quantitative proteomics and thermal proteome profiling on MIA PaCa-2 cells. This study revealed that HCQ may exert its functions by targeting some autophagy-related proteins such as ribosyldihydronicotinamide dehydrogenase (NQO2) and transport protein Sec23A (SEC23A), or regulating the expression of galectin-8 (LGALS8), mitogen-activated protein kinase 8 (MAPK8), and so on. Furthermore, HCQ may prevent the progression of pancreatic cancer by regulating the expression of nesprin-2 (SYNE2), protein-S-isoprenylcysteine O-methyltransferase (ICMT), and cotranscriptional regulator FAM172A (FAM172A). Together, these findings not only identified potential binding targets for HCQ but also revealed the non-canonical mechanisms of HCQ that may contribute to pancreatic cancer treatment.
关键词	hydroxychloroquine quantitative proteomics thermal proteome profiling
URL	查看原文
收录类别	SCI ; SCIE
语种	英语
资助项目	National Natural Science Foundation of China[81973164]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:000845365500001
出版者	MDPI
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/226382
专题	生命科学与技术学院_硕士生
通讯作者	Chen, Kaixian; Huang, He
作者单位	1.China Pharmaceut Univ, Sch Pharm, Nanjing 211198, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 4.Northeastern Univ, Sch Mech Engn & Automat, Shenyang 110819, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Jingxiang,Zhao, Zhiqiang,Hou, Wanting,et al. Quantitative Proteomics Explore the Potential Targets and Action Mechanisms of Hydroxychloroquine[J]. MOLECULES,2022,27(16).
APA	Zhao, Jingxiang.,Zhao, Zhiqiang.,Hou, Wanting.,Jiang, Yue.,Liu, Guobin.,...&Huang, He.(2022).Quantitative Proteomics Explore the Potential Targets and Action Mechanisms of Hydroxychloroquine.MOLECULES,27(16).
MLA	Zhao, Jingxiang,et al."Quantitative Proteomics Explore the Potential Targets and Action Mechanisms of Hydroxychloroquine".MOLECULES 27.16(2022).