Suppressor of Deltex mediates Pez degradation and modulates Drosophila midgut homeostasis

doi:10.1038/ncomms7607

	Suppressor of Deltex mediates Pez degradation and modulates Drosophila midgut homeostasis
	Wang, Chao 1; Zhang, Wenxiang 1; Yin, Meng-Xin 1; Hu, Lianxin 1; Li, Peixue 1; Xu, Jiajun 1; Huang, Hongling 2; Wang, Shimin 1; Lu, Yi 1; Wu, Wenqing 1; Ho, Margaret S.3; Li, Lin 4; Zhao, Yun1,5 ; Zhang, Lei1,5
	2015-03
发表期刊	NATURE COMMUNICATIONS
ISSN	2041-1723
卷号	6
发表状态	已发表
DOI	10.1038/ncomms7607
摘要	Pez functions as an upstream negative regulator of Yorkie (Yki) to regulate intestinal stem cell (ISC) proliferation and is essential for the activity of the Hippo pathway specifically in the Drosophila midgut epithelium. Here we report that Suppressor of Deltex (Su(dx)) acts as a negative regulator of Pez. We show that Su(dx) targets Pez for degradation both in vitro and in vivo. Overexpression of Su(dx) induces proliferation in the fly midgut epithelium, which can be rescued by overexpressed Pez. We also demonstrate that the interaction between Su(dx) and Pez, bridged by WW domains and PY/PPxY motifs, is required for Su(dx)-mediated Pez degradation. Furthermore, we find that Kibra, a binding partner of Pez, stabilizes Pez via WW-PY/PPxY interaction. Moreover, PTPN14, a Pez mammalian homolog, is degraded by overexpressed Su(dx) or Su(dx) homologue WWP1 in mammalian cells. These results reveal a previously unrecognized mechanism of Pez degradation in maintaining the homeostasis of Drosophila midgut.
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31171394] ; National Natural Science Foundation of China[31371462]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000353040500002
出版者	NATURE PUBLISHING GROUP
WOS关键词	STEM-CELL PROLIFERATION ; UBIQUITIN LIGASE ; BREAST-CANCER ; HIPPO PATHWAY ; TGF-BETA ; PROTEIN ; GENE ; REGULATOR ; WWP1 ; DOMAINS
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2250
专题	生命科学与技术学院_特聘教授组_赵允组生命科学与技术学院_特聘教授组_张雷组
通讯作者	Zhang, Lei
作者单位	1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, Shanghai 200031, Peoples R China 2.VIB, Lab Angiogenesis & Neurovasc Link, Vesalius Res Ctr, B-3000 Leuven, Belgium 3.Tongji Univ, Sch Med, Dept Anat & Neurobiol, Shanghai 200092, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China 5.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 200120, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Wang, Chao,Zhang, Wenxiang,Yin, Meng-Xin,et al. Suppressor of Deltex mediates Pez degradation and modulates Drosophila midgut homeostasis[J]. NATURE COMMUNICATIONS,2015,6.
APA	Wang, Chao.,Zhang, Wenxiang.,Yin, Meng-Xin.,Hu, Lianxin.,Li, Peixue.,...&Zhang, Lei.(2015).Suppressor of Deltex mediates Pez degradation and modulates Drosophila midgut homeostasis.NATURE COMMUNICATIONS,6.
MLA	Wang, Chao,et al."Suppressor of Deltex mediates Pez degradation and modulates Drosophila midgut homeostasis".NATURE COMMUNICATIONS 6(2015).