Improving high carbon dioxide tolerance and carbon dioxide fixation capability of Chlorella sp by adaptive laboratory evolution

doi:10.1016/j.biortech.2015.03.011

	Improving high carbon dioxide tolerance and carbon dioxide fixation capability of Chlorella sp by adaptive laboratory evolution
	Li, Dengjin 1,2; Wang, Liang 1; Zhao, Quanyu1,3 ; Wei, Wei1,3 ; Sun, Yuhan1,3
	2015-06
发表期刊	BIORESOURCE TECHNOLOGY (IF:9.7[JCR-2023],9.4[5-Year])
ISSN	0960-8524
卷号	185 页码:269-275
发表状态	已发表
DOI	10.1016/j.biortech.2015.03.011
摘要	CO2 capture by microalgae is a promising method to reduce greenhouse gas emissions. It is critical to construct a highly efficient way to obtain a microalgal strain tolerant to high CO2 concentrations with high CO2 fixation capability. In this study, two evolved Chlorella sp. strains, AE10 and AE20 were obtained after 31 cycles of adaptive laboratory evolution (ALE) under 10% and 20% CO2, respectively. Both of them grew rapidly in 30% CO2 and the maximal biomass concentration of AE10 was 3.68 +/- 0.08 g/L, which was 1.22 and 2.94 times to those of AE20 and original strain, respectively. The chlorophyll contents of AE10 and AE20 were significantly higher than those of the original one under 1-30% CO2. The influences of ALE process on biochemical compositions of Chlorella cells were also investigated. This study proved that ALE was an effective approach to improve high CO2 tolerance of Chlorella sp. (C) 2015 Elsevier Ltd. All rights reserved.
关键词	Adaptive laboratory evolution High CO2 concentration tolerance Chlorella sp. CO2 fixation
收录类别	SCI ; EI
语种	英语
资助项目	Science and Technology Commission of Shanghai Municipality Program[13DZ1205000] ; Science and Technology Commission of Shanghai Municipality Program[14DZ1203800]
WOS研究方向	Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS类目	Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS记录号	WOS:000353190500036
出版者	ELSEVIER SCI LTD
EI入藏号	20151100647375
EI主题词	Carbon ; Gas emissions ; Greenhouse gases ; Strain
EI分类号	Strength of Building Materials; Mechanical Properties:421 ; Air Pollution Sources:451.1 ; Chemical Products Generally:804 ; Inorganic Compounds:804.2 ; Materials Science:951
WOS关键词	LIPID PRODUCTION ; FLUE-GAS ; BIOMASS PRODUCTION ; BIO-MITIGATION ; CO2 ; VULGARIS ; SEQUESTRATION ; PRODUCTIVITY ; PYRENOIDOSA ; ENHANCEMENT
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2208
专题	生命科学与技术学院_特聘教授组_赵权宇组物质科学与技术学院_特聘教授组_孙予罕组物质科学与技术学院_特聘教授组_魏伟组
通讯作者	Zhao, Quanyu
作者单位	1.Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai 201210, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.ShanghaiTech Univ, Shanghai 200031, Peoples R China
通讯作者单位	上海科技大学
推荐引用方式 GB/T 7714	Li, Dengjin,Wang, Liang,Zhao, Quanyu,et al. Improving high carbon dioxide tolerance and carbon dioxide fixation capability of Chlorella sp by adaptive laboratory evolution[J]. BIORESOURCE TECHNOLOGY,2015,185:269-275.
APA	Li, Dengjin,Wang, Liang,Zhao, Quanyu,Wei, Wei,&Sun, Yuhan.(2015).Improving high carbon dioxide tolerance and carbon dioxide fixation capability of Chlorella sp by adaptive laboratory evolution.BIORESOURCE TECHNOLOGY,185,269-275.
MLA	Li, Dengjin,et al."Improving high carbon dioxide tolerance and carbon dioxide fixation capability of Chlorella sp by adaptive laboratory evolution".BIORESOURCE TECHNOLOGY 185(2015):269-275.